Isothermal Microcalorimetry

Gaisford, Simon

doi:10.1007/978-1-4939-4029-5_12

Cited by 4 publications

(2 citation statements)

References 70 publications

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“…As a prerequisite for thermobiology investigations, measuring cell temperature poses a challenge for traditional thermometry. Originally intended for bulk and homogeneous materials, calorimetry lacks the spatial resolution to visualize microscopic temperature distributions. , Electric probes have slow response because of high thermal boundary resistance …”

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confidence: 99%

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer

Wazawa

Sakamoto

et al. 2022

Nano Lett.

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Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 μs and a temperature resolution 0.042 °C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions.

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confidence: 99%

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer

Wazawa

Sakamoto

et al. 2022

Nano Lett.

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“…The calorimeter is one of the earliest thermodynamic analysis instruments, which has been used in the field of biological research . The calorimeter was first developed by Lavoisier and Laplace dates back to 1780 .…”

Section: Thermo Sensors For Biologymentioning

confidence: 99%

Cell Temperature Measurement for Biometabolism Monitoring

Wang

Han

2020

ACS Sens.

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Temperature is an important factor in the process of life, as thermal energy transfer participates in all biological events in organisms. Due to technical limitations, there is still a lot more information to be explored regarding the correlation between life activities and temperature changes. In recent years, the emergence of a variety of new temperature measurement methods has facilitated further research in this field. Here, we introduce the latest advances in temperature sensors for biological detection and their related applications in metabolic research. Various technologies are discussed in terms of their advantages and shortcomings, and future prospects are presented.

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Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study

Kattel,

Aro,

Lahtvee

et al. 2024

MicrobiologyOpen

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The gut microbiota significantly contributes to human health and well‐being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next‐generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of Akkermansia muciniphila, Bacteroides thetaiotaomicron, and Faecalibacterium prausnitzii were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (A. muciniphila), Bacteroides thetaiotaomicron (B. thetaiotaomicron), and Faecalibacterium prausnitzii (F. prausnitzii) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next‐generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.

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Isothermal Microcalorimetry

Cited by 4 publications

References 70 publications

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer

Cell Temperature Measurement for Biometabolism Monitoring

Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study

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