2024
DOI: 10.1021/acs.analchem.4c00056
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Isotope-Edited Variable Temperature Infrared Spectroscopy for Measuring Transition Temperatures of Single A-T Watson–Crick Base Pairs in DNA Duplexes

Hao-Che Peng,
Shrijaa Mohan,
Muhammad T. Huq
et al.

Abstract: Experimental methods to determine transition temperatures for individual base pair melting events in DNA duplexes are lacking despite intense interest in these thermodynamic parameters. Here, we determine the dimensions of the thymine (T) C2O stretching vibration when it is within the DNA duplex via isotopic substitutions at other atomic positions in the structure. First, we determined that this stretching state was localized enough to specific atoms in the molecule to make submolecular scale measurements of … Show more

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