Isozyme differentiation among 17 geographical isolates of Hirsutella thompsonii

Boucias, Drion G.; McCoy, C. W.; Joslyn, D. J.

doi:10.1016/0022-2011(82)90057-x

Cited by 19 publications

(8 citation statements)

References 9 publications

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“…Clade III isolates were all HtA -and were isolated from Thailand, albeit from different locations and hosts. The fact that H. thompsonii isolates are difficult to identify is due to the pleomorphic character of this fungus and the high polymorphism detected among different isolates in regard to several biological characteristics and molecular markers (Boucias et al, 1982, Mozes-Koch et al, 1995, Van der Geest et al, 2000. Our results have confirmed that the taxonomy of Hirsutella is complex (Samson et al, 1988) and that genomic characterization, through the analysis of other DNA sequences, should be developed in order to refine the taxonomy of H. thompsonii.…”

Section: Discussionmentioning

confidence: 99%

“…Isoenzyme analyses of 15 geographically diverse H. thompsonii isolates has shown a high degree of intra-specific variability even among morphologically similar Hirsutella isolates (Boucias et al, 1982). Maimala et al (2002) reported that the major hosts of H. thompsonii are eriophyoid mites displaying specific host plant preferences and that many H. thompsonii isolated from a range of eriophyiids varied in their ability to produce exotoxins such as Hirsutellin A (HtA).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences

et al. 2006

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Amplified fragment length polymorphism (AFLP) was used to investigate polymorphism among 43 Hirsutella thompsonii isolates (33 from Thailand) obtained from various mite species. The outgroups were an unidentified Hirsutella isolate along with Hirsutella nodulosa and Hirsutella kirchneri. Phylogenetic analyses of the AFLP data showed significant variation among isolates and the existence of three H. thompsonii clades. We also investigated the isolates using PCR with specific primers for the Hirsutella exotoxin gene Hirsutelin A (HtA) and 18 of these isolates were used for sequencing of the partial b-tubulin gene. Phylogenetic analyses of b-tubulin sequences showed two distinct H. thompsonii clades, one of which included AFLP clades I and II. For both markers grouping of the H. thompsonii isolates was not related to either host mite species or geographical origin, although for the HtA gene one clade contained only isolates with no detectable HtA band. These results confirm the high intraspecific polymorphism of H. thompsonii, and maximum likelihood analysis showed no monophyletic group within this species. To refine the taxonomy of this genus other studies should be undertaken using additional molecular markers and several other Hirsutella isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences

et al. 2006

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“…According to this analysis HtCRMC is closely related to HtCRMK1 and HtCRMK2, while HtPMG is related to HtCRMB. These results suggest that the differentiation of the RAPD pattern may not be immediately associated with morphological characteristics (Boucias et al, 1982). …”

Section: Resultsmentioning

confidence: 76%

“…The results of an isozyme analysis of 17 distinct geographical isolates of H. thompsonii suggested that each of the isolates possesses a distinct isozyme pattern (Boucias et al, 1982) which, based on coefficients of similarity data for the isozyme patterns, closely follows the morphological traits on which the identification of the three H. thompsonii varieties is based. The results also revealed that the isozyme patterns of the non-synnematous vinaceous varieties of H. thompsonii closely resemble those of the non-synnematous grayish-green varieties.…”

Section: Introductionmentioning

confidence: 98%

Genetic diversity in Hirsutella thompsonii isolates based on random amplified polymorphic DNA analysis

Aghajanzadeh¹,

Prasad²,

Mallik³

2006

BioControl

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Hirsutella thompsonii Fisher is a host-specific fungal pathogen of the diverse group of arthropods known as the Acari. It particularly affects eriophyid mites, and the identification of its isolates is a difficult task due to its pleomorphic nature. Seven isolates of H. thompsonii var. thompsonii and H. thompsonii var. synnematosa collected from various agro-climatic regions of India were subjected to PCR analysis using random decamer primers to differentiate between them. Random amplified polymorphic DNA analysis revealed that all of the isolates from the citrus rust mite, Phyllocoptruta oleivora Ashmead (Acari: Eriophyidae), clustered together, with the exception of one, HtCRMB. The only isolate of H. thompsonii from Aceria guerreronis keifer (Acari: Eriophydae), HtPDBC, clustered separately.

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“…The former fungus is known to be very pleomorphic, with many isolates collected and identiWed from diVerent parts of the world (Boucias et al 1982;Mozes-Koch et al 1995;Aghajanzadeh et al 2007). Such isolates may arise through geographical isolation but also by anastomosis (unpublished data).…”

Section: Discussionmentioning

confidence: 93%

A tale of three acaropathogenic fungi in Israel: Hirsutella, Meira and Acaromyces

et al. 2008

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We review published and unpublished studies conducted in Israel with six acaropathogenic fungi, assayed in order to control the citrus rust mite, Phyllocoptruta oleivora (Ashmead) (CRM). Hirsutella thompsonii Fisher was introduced twice, killed 80-90% of the exposed mites, but due to its requirements for near-saturation humidities was deemed unsuitable for local outdoors conditions. Hirsutella kirchneri (Rostrup) Minter et al. and Hirsutella necatrix Minter et al. were also introduced and assayed against CRM and spider mites, but their efficacy was unsatisfactory. Three indigenous fungi found to be associated with mites, Meira geulakonigii, Meira argovae and Acaromyces ingoldii--all three recently described by Boekhout, Gerson, Scorzetti & Sztejnberg--were assayed against several mites. Meira geulakonigii killed 80-90% of several spider mites and of the CRM, and caused some mortality of Iphiseius degenerans (Berlese), one out of three phytoseiid predators assayed. Mortality was not due to parasitization; extracts from the media in which the fungi had developed caused considerable mite death, suggesting that it was a result of fungal toxins. Data from a field study indicated that spraying blastoconidia of M. geulakonigii on grapefruits infested by CRM significantly reduced pest-incurred damage from 23 to 13%. Applying qRT-PCR methodology indicated that M. geulakonigii was endophytic within sealed grapefruit flowers and in the flavedo of the fruits' peel. Neither in the laboratory nor in the field was any evidence ever obtained that this fungus damaged the plants, leading us to hypothesize that M. geulakonigii serves as a "body guard" of grapefruits (and perhaps other plants as well). All three fungi suffered very little mortality after being exposed to various insecticides and acaricides that are in current local use (with the exception of sulfur). The ability of M. geulakonigii to reduce mite numbers without affecting the host plant, the minimal fungal effect on some predatory mites, its endophytic nature along with the apparent tolerance of M. geulakonigii to many insecticides and acaricides, suggest that this fungus could be suitable for integrated pest management (IPM) program.

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Isozyme differentiation among 17 geographical isolates of Hirsutella thompsonii

Cited by 19 publications

References 9 publications

Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences

Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences

Genetic diversity in Hirsutella thompsonii isolates based on random amplified polymorphic DNA analysis

A tale of three acaropathogenic fungi in Israel: Hirsutella, Meira and Acaromyces

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