Isozyme evidence for two groups of Fusarium graminearum

Láday, M.; Bagi, Ferenc; Mesterházy, Ákos; Szécsi, Á.

doi:10.1017/s0953756299002361

Cited by 22 publications

(13 citation statements)

References 28 publications

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“…Native enzymes were extracted from lyophilised and powdered mycelia of Phytophthora (25 mg) into buffer (350 µl), and the extract was centrifuged (16 000 g , 10 min) 15. Aliquots (1.5 µl) of the supernatant were then subjected to electrophoresis on cellulose acetate gels according to the manufacturer's instructions 16.…”

Section: Methodsmentioning

confidence: 99%

Standard and Swedish variant types of the hybrid alder Phytophthora attacking alder in Hungary

Nagy

Bakonyi

Érsek

2003

Pest Management Science

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A new Phytophthora disease of common alder (Alnus glutinosa) similar to that previously reported in several countries in Europe has been observed in Hungary. Based on these earlier studies, the alder Phytophthora was considered likely to be a hybrid between P cambivora and a P fragariae-like species: across Europe a range of new alder Phytophthora is spreading that comprise a range of heteroploid hybrids including a 'standard' hybrid type and several other hybrid types termed 'variants'. Phenotypic and molecular features of the pathogen in Hungary were characterised and compared with isolates from elsewhere. The morphologies of five isolates from one region (Hévíz) resembled the common, 'standard' type, whereas the three isolates from another region (Hanság) exhibited traits similar to those of one of the 'variant' types, ie the Swedish 'variant'. Molecular markers of these two groups of Hungarian isolates also represented a good fit to those of the standard type and the Swedish variant, respectively. Isozyme patterns and profiles of restriction fragments of the entire internal transcribed spacer (ITS) region or mitochondrial DNAs and of RAPD-PCR products did not differ within a group, but distinct polymorphisms were exhibited between the two groups of isolates. Southern analysis of random amplified polymorphic DNA (RAPD) revealed the homologous nature of co-migrating bands of P cambivora and the isolates of alder Phytophthora. Furthermore, restriction fragment profiles of the ITS region of ribosomal DNAs and the mtDNAs were consistent with reported biparental origin of alder Phytophthora. The hybrid status of these continuously evolving pathogens raises many issues and challenges concerning efficient control measures.

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Section: Methodsmentioning

confidence: 99%

Standard and Swedish variant types of the hybrid alder Phytophthora attacking alder in Hungary

Nagy

Bakonyi

Érsek

2003

Pest Management Science

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“…Four groups were defined, which were suggested to represent four different species based on their genetic distances. Using 26 enzyme systems, Láday et al (2000) investigated polymorphism among 34 isolates of Fusarium graminearum. The results obtained classified the enzymes into two different groups based on their respective geographic origins.…”

Section: Evaluation Of Genetic Diversity Of Endophyte Isolates Based mentioning

confidence: 99%

“…Isoenzyme analysis has been successfully used to identify fungal species and in the differentiation of phytopathogenic fungi (Goodwin et al, 1995;Smith and Sivasithamparam, 2000;Láday et al, 2000). This technique provides a reliable method for the characterization and differentiation of endophytic fungi and their phytopathogens that is relatively fast, sensitive, and specific, without the need for further genetic or morphological analyses.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses

Leme¹,

Bevilaqua²,

Rhoden³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as rDNA markers. Thirty-two esterases were obtained from 37 isolates of Saccharum spp, which clustered into five endophyte groups. Esterase EST-06 was observed with the highest frequency, being present in 22 of the 37 isolates analyzed, followed by esterase EST-11, which was present in 20 isolates. The esterases EST-10 and EST-14 were present in 19 isolates and EST-09 was present in 18 isolates. The esterase EST-01 was unique to isolate 33 and can, therefore, be used as a marker for this isolate. None of the esterases identified were common to all isolates tested. Similarly, phylogenetic analysis, based on rDNA sequence data, classified the isolates into 5 genus groups: 1) Curvularia with a 100% bootstrap value (BP), 2) Alternaria with 100% BP, 3) Epicoccum with 60% BP, 4) Phoma with 89% BP, and 5) Saccharicola with 100% BP. This polyphyletic analysis based on several markers, therefore, proved to be a valuable approach in determining the relationship between variation in endophytes and their associated host plants. Furthermore, both the esterase and rDNA analyses obtained similar results and were equally effective in resolving relationships.

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“…graminearum Group 1 (Burgess et al 1975). Based on variation in DNA sequence (Aoki & O'Donnell 1999a), RFLP (Benyon et al 2000) and isozymes (Laday et al 2000), F. pseudograminearum has been formally accepted as a separate species. A key factor differentiating the two species is the heterothallic nature of F. pseudograminearum (teleomorph: Gibberella coronicola) compared to the homothallic F. graminearum (teleomorph: Gibberella zeae) (Aoki & O'Donnell 1999b).…”

Section: A U T H O R ' S P E R S O N a L C O P Ymentioning

confidence: 99%

Multilocus sequence analysis of Fusarium pseudograminearum reveals a single phylogenetic species

Scott

Chakraborty

2006

Mycological Research

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Isozyme evidence for two groups of Fusarium graminearum

Cited by 22 publications

References 28 publications

Standard and Swedish variant types of the hybrid alder Phytophthora attacking alder in Hungary

Standard and Swedish variant types of the hybrid alder Phytophthora attacking alder in Hungary

Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses

Multilocus sequence analysis of Fusarium pseudograminearum reveals a single phylogenetic species

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