2010
DOI: 10.1111/j.1365-2141.2009.08012.x
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ITD‐ and FL‐induced FLT3 signal transduction leads to increased C/EBPβ‐LIP expression and LIP/LAP ratio by different signalling modules

Abstract: Summary FLT3 receptor‐associated signalling plays a role in proliferation and leukaemia. The transcription factor C/EBPβ may be involved in malignancy with its alternative translation product C/EBPβ‐LIP. We investigated a potential connection between FLT3 signalling and the C/EBPβ system in FLT3‐internal tandem duplication (ITD)‐positive leukaemia cells and FLT3‐ITD‐ or FLT3‐wild type (WT)‐transfected 32D cells. In FLT3‐ITD‐positive cells or when ITD sequences were inserted into the FLT3‐WT receptor, significa… Show more

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Cited by 22 publications
(23 citation statements)
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“…Cell lysis, total RNA isolation, validation of RNA concentration/purity, and generation of external standard curves are described elsewhere (24). Two μg of total RNA were reverse-transcribed as described previously (25).…”
Section: Methodsmentioning
confidence: 99%
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“…Cell lysis, total RNA isolation, validation of RNA concentration/purity, and generation of external standard curves are described elsewhere (24). Two μg of total RNA were reverse-transcribed as described previously (25).…”
Section: Methodsmentioning
confidence: 99%
“…Expression of human IL-8 mRNA was assessed by real-time RT-PCR using a LightCycler 480, universal probe library probe 72 (Roche Applied Science), and specific primers (5′-AGACAGCAGAGCACACAAGC-3′ and 5′-ATGGTTCCTTCCGGTGGT-3′). For the amplification, a standard protocol for experiments using universal probe library probes was used (24). Normalization of cDNA amounts was performed as described (19).…”
Section: Methodsmentioning
confidence: 99%
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“…28 Furthermore, expression of LIP is increased in FMS-like tyrosine kinase 3 (FLT3)-internal tandem duplication (ITD)-positive myeloid leukemic cells. 29 On the other hand, C/EBPb was shown to be upregulated during ATRAinduced differentiation of acute promyelocytic leukemia cells, and it was considered as a potential therapeutic target in leukemia for granulocytic differentiation. 30 Taken together, C/EBPb has leukemia suppressor-like activity in acute promyelocytic leukemia, and it also has critical oncogenic functions in FLT3-ITD leukemia, B-lineage acute lymphoblastic leukemia, and multiple myeloma.…”
Section: Discussionmentioning
confidence: 99%
“…Subsequently, the LIP:LAP ratio was calculated by dividing the LIP value by the respective combined LAP1 and LAP2 value (together denoted as LAP in the LIP:LAP ratio) as previously described (Haas et al, 2010).…”
Section: Calculation Of Lip:lap Ratiosmentioning
confidence: 99%