iTRAQ and PRM-based comparative proteomic profiling in gills of white shrimp Litopenaeus vannamei under copper stress

Guo, Hui; Chen, Tianci; Liu, Zhi; Fan, Lanfen; Yuchun, Shen; Zhou, Dayan

doi:10.1016/j.chemosphere.2020.128270

Cited by 29 publications

(15 citation statements)

References 84 publications

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“…37 Cathepsin L shows a negative correlation with the texture of the muscle, which could be explained by the fact that cathepsin L has a responsibility for the textural changes and myofibrillar protein degradation in the postmortem muscles of PNVN muscles. Guo et al reported that cathespin L was upregulated in Litopenaeus vannamei after Cu stress, 38 which is in agreement with the proteomic response of Mytilus galloprovincialis under the treatment of CuO NPs and Cu 2+ . 39 Liu et al also reported that cathepsin L has a capacity to destroy the structure of silver carp surimi gels and soften gels.…”

Section: ■ Results and Discussionsupporting

confidence: 63%

Differential Proteomics Analysis of Penaeus vannamei Muscles with Quality Characteristics by TMT Quantitative Proteomics during Low-Temperature Storage

Wang

Pang

Peng

2021

J. Agric. Food Chem.

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A tandem mass tag technology, based on the quantitative proteomics strategy, was applied to investigate the relationships between proteome changes of Penaeus vannamei (PNVN) muscles and quality characteristics during low-temperature storage. 506 proteins were found as differentially expressed proteins (DEPs) after 10 days of storage under treatments of refrigerated storage (5 °C), ice temperature storage (0 °C), and particle freezing storage (−3 °C) compared with a fresh group (0 day). In addition, Uniprot Knowledgebase (UniprotKB), Gene Ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes were reported. Correlation analysis indicated that nine DEPs were significantly related to quality characteristicspH, color, and texture. Bioinformatics analysis showed that most of DEPs were involved in binding proteins, metabolic enzyme, and protein turnover. Besides, several DEPs could be good candidate biomarkers of muscle decline. These results could help to further comprehend the proteome changes and mechanisms of the quality decline of PNVN muscles during low-temperature storage.

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Section: ■ Results and Discussionsupporting

confidence: 63%

Differential Proteomics Analysis of Penaeus vannamei Muscles with Quality Characteristics by TMT Quantitative Proteomics during Low-Temperature Storage

Wang

Pang

Peng

2021

J. Agric. Food Chem.

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“…31 Significant changes in the CYP450 enzyme series were observed in a series of proteomic studies conducted under toxic stress. [32][33][34][35] However, none of the 274 differential proteins in this study were identified as CYP450. We speculated that OA, as a widely existing substance in plants that is involved in stress responses, could not activate metabolic reactions of CYP450 and other detoxification enzymes in snails, so that it could perform the corresponding physiological activities.…”

Section: Discussionmentioning

confidence: 80%

iTRAQ‐based quantitative proteomic analysis in liver of Pomacea canaliculata induced by oleanolic acid stress

Cen

Chen

et al. 2022

Pest Management Science

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Background Triterpene acid is one of the typical active constituents of Eucalyptus bark, which is the main by‐product of the Eucalyptus wood industry. Our studies have demonstrated that triterpene acid stress could inhibit climbing and increase mortality in Pomacea canaliculata (Lamarck). However, limited attention has been paid to the proteomic responses of this snail under triterpene acid stress. Result Using iTRAQ‐based quantitative proteomics, we elucidated the regulatory mechanism in the livers of P. canaliculata held in chlorine‐free water and exposed to 100 mg L−1 oleanolic acid (OA) for 24 h. A total of 4308 proteins were identified, of which 274 were differentially expressed proteins (DEPs) including 168 (61.31%) differentially upregulated proteins and 106 (38.69%) differentially downregulated proteins. Bioinformatics analysis revealed that P. canaliculata responses to OA stress are mainly involved in glucose metabolism, energy synthesis, immune response, stress response, protein synthesis, and apoptosis. According to KEGG analysis, the 274 DEPs were mapped to 168 KEGG pathways and 10 KEGG pathways were significantly enriched (P < 0.05). Furthermore, qRT‐PCR was performed for histone H4, catalase, isocitrate dehydrogenase, superoxide dismutase, ferritin, lipase, and tropomyosin to validate the iTRAQ results. Conclusion Proteomic analysis suggested that OA stress led to the disruption of glucose metabolism, energy synthesis, and protein synthesis, and triggered a series of molecular pathways containing many key proteins involved in the immune process, thereby helping P. canaliculata resist OA stress. © 2022 Society of Chemical Industry.

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“…A total of three fetal rats were obtained from each pregnant rat, for a total of 54 fetal rats from both GA16.5 and GA18.5 groups, and the survival rate was calculated. Tissue samples from the surgical site on the upper-left lip and asymmetrical sections from the upper-right lip were collected from the fetal rats for histological examination, including hematoxylin and eosin (H&E) staining, Masson's Trichrome staining and type-I collagen immunohistochemical staining as previously described ( 14 – 16 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of differentially expressed proteins involved in fetal scarless wound healing using a rat model of cleft lip

Liu

et al. 2021

Mol Med Rep

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in early pregnancy, fetal skin wounds can heal quickly and undergo a transition period from scarless healing to scar formation. The aim of the present study was to identify potential biomarkers associated with scarless repair of cleft lips, in order to determine the intrinsic factors leading to scar formation in embryonic tissue. a stable model of cleft lip was established using microsurgery by constructing a wedge-shaped cleft lip-like defect in fetal rats at gestational age (Ga) 16.5 and Ga18.5. The Ga16.5 and Ga18.5 groups were used to model scarless healing and scar formation, respectively. The fetuses were returned to the uterus following surgery, then removed 72 h after the procedure. Macroscopic observation of the cleft defect and histological examination were carried out. reverse transcription-quantitative (rT-q) Pcr and parallel reaction monitoring (PrM) were used to detect mrna and protein expression levels, respectively. The upper-left lip completely healed 72 h after surgery in the Ga16.5 group of fetal rats. However, this was not the case in the Ga18.5 group. Histological examination indicated new follicles visible under the epidermis of the scarless group (Ga16.5). Scarring was visible on the upper-left cleft lip wound of the fetal rats in the Ga18.5 group. The expression of some growth and pro-inflammatory factors, including TnF-α, were also different between two groups. Label-free quantification was used to identified differentially expressed proteins and five differentially expressed proteins (Smad4, Fabp5, S100a4, S100a8 and S100a9) were identified. The relative expression of these molecules at the mrna and protein levels were measured using rT-qPcr and PrM. These molecules may represent potential biomarkers for the scarless repair of fetal rat cleft lip wounds.

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iTRAQ and PRM-based comparative proteomic profiling in gills of white shrimp Litopenaeus vannamei under copper stress

Cited by 29 publications

References 84 publications

Differential Proteomics Analysis of Penaeus vannamei Muscles with Quality Characteristics by TMT Quantitative Proteomics during Low-Temperature Storage

Differential Proteomics Analysis of Penaeus vannamei Muscles with Quality Characteristics by TMT Quantitative Proteomics during Low-Temperature Storage

iTRAQ‐based quantitative proteomic analysis in liver of Pomacea canaliculata induced by oleanolic acid stress

Identification of differentially expressed proteins involved in fetal scarless wound healing using a rat model of cleft lip

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