iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

Liu, Lu; Hao, Liaoyang; Liu, Ning; Zhao, Yong‐Long; Zhong, Nanshan; Zhao, Pan

doi:10.3390/ijms222112036

Cited by 3 publications

(1 citation statement)

References 89 publications

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“…For example, the ethylene response factor TaERF87 , a new gene for drought resistance in wheat [ 3 ], and the polyamine oxidase OsPAO5 , a new gene for rice mesocotyl elongation [ 4 ], were identified by RNA-seq technology. However, compared with RNA, protein is the direct controller of biochemical processes in organisms, so added protein quantitative techniques, mainly isobaric tags for relative and absolute quantification (iTRAQ) [ 5 , 6 ] and (tandem mass tag) TMT [ 7 , 8 ] techniques, have been applied in plant research. In addition, combining transcriptome and proteome analysis is the best method to analyze gene expression comprehensively and deeply [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (Oryza sativa L.)

Guo

Tang

Wang

et al. 2023

IJMS

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Dormancy is a complex agronomy phenotype controlled by multiple signaling and a key trait repressing pre-harvest sprouting (PHS). However, the signaling network of dormancy remains unclear. In this study, we used Zhonghua11 (ZH11) with a weak dormancy, and Introgression line (IL) with a strong dormancy to study the mechanism of hormones and reactive oxygen species (ROS) crosstalk regulating rice dormancy. The germination experiment showed that the germination rate of ZH11 was 76.86%, while that of IL was only 1.25%. Transcriptome analysis showed that there were 1658 differentially expressed genes (DEGs) between IL and ZH11, of which 577 were up-regulated and 1081 were down-regulated. Additionally, DEGs were mainly enriched in oxidoreductase activity, cell periphery, and plant hormone signal transduction pathways. Tandem mass tags (TMT) quantitative proteomics analysis showed 275 differentially expressed proteins (DEPs) between IL and ZH11, of which 176 proteins were up-regulated, 99 were down-regulated, and the DEPs were mainly enriched in the metabolic process and oxidation-reduction process. The comprehensive transcriptome and proteome analysis showed that their correlation was very low, and only 56 genes were co-expressed. Hormone content detection showed that IL had significantly lower abscisic acid (ABA) contents than the ZH11 while having significantly higher jasmonic acid (JA) contents than the ZH11. ROS content measurement showed that the hydrogen peroxide (H2O2) content of IL was significantly lower than the ZH11, while the production rate of superoxide anion (O2.−) was significantly higher than the ZH11. These results indicate that hormones and ROS crosstalk to regulate rice dormancy. In particular, this study has deepened our mechanism of ROS and JA crosstalk regulating rice dormancy and is conducive to our precise inhibition of PHS.

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Section: Introductionmentioning

confidence: 99%

Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (Oryza sativa L.)

Guo

Tang

Wang

et al. 2023

IJMS

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Pathogenic Factors of Plant Pathogenic Streptomyces

Zhang,

Ping,

Liu

et al. 2023

Potato Res.

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The OsGAPC3 mutation significantly affects grain quality traits and improves the nutritional quality of rice

Peng,

Liu,

Sun

et al. 2024

Front. Plant Sci.

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The glycolytic enzyme cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GAPC3) is involved in multiple biological processes in plants, including transcriptional regulation, and material metabolism. However, the relationship between OsGAPC3 and the quality traits of rice is poorly understood. Here we identify OsGAPC3 mutations that enhance the protein content and grain nutritional quality of rice by regulating the OsAAP6 gene expression. The number and volume of type-II protein bodies in the endosperm of the OsGAPC3 mutants, and GPC increase significantly. We report significant increases in chalkiness area and degree, and decreases for starch content, gel consistency, and taste value. Results of proteomic detection and analysis reveal that OsGAPC3 affects the major storage substances (proteins and starch) metabolism in rice, and the accumulation of proteins and starch in the endosperm. Additionally, the OsGAPC3 mutation significantly decreases the rice-seedling salt tolerance. Therefore, OsGAPC3 affects multiple quality traits of rice, participates in regulating rice-seedling salt-stress response. These data can be used to design better-quality and stronger salt-resistant rice varieties.

show abstract

iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

Cited by 3 publications

References 89 publications

Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (Oryza sativa L.)

Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (Oryza sativa L.)

Pathogenic Factors of Plant Pathogenic Streptomyces

The OsGAPC3 mutation significantly affects grain quality traits and improves the nutritional quality of rice

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