Ixodes inopinatus − Occurring also outside the Mediterranean region

Chiţimia-Dobler, Lidia; Rieß, Ramona; Kahl, Olaf; Wölfel, Silke; Dobler, Gerhard; Nava, Stefano; Estrada-Peña, Agustı́n

doi:10.1016/j.ttbdis.2017.09.004

Cited by 71 publications

(43 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(n = 58, mean ticks ± SE = 5.8 ± 0.79 randomly selected per country) using a conventional PCR targeting the mitochondrial 16S rRNA gene of ticks using the primers described by (Mangold, Bargues, & Mas-Coma, 1998) and an annealing temperature of 56°C, to confirm morphological identification of ticks by BLASTn search (https ://blast.ncbi.nlm.nih.gov/). For specimens (n = 4) for which the % of identity in BLASTn search was less than 98% we built a Maximum Likelihood phylogenetic tree, together with reference sequences (Chitimia-Dobler et al, 2018;Estrada-Peña et al, 2014) retrieved from Genbank, and confirmed the species identification with the obtained clustering patterns. We assessed infection of ticks by a nested PCR targeting the flaB gene of B. burgdorferi s.l.…”

Section: Molecular Analysismentioning

confidence: 96%

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Norte

Margos²,

Becker

et al. 2020

Molecular Ecology

View full text Add to dashboard Cite

Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick‐borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevalence (92 and 58%, respectively), whereas robin Erithacus rubecula ticks were the least infected (3.8%). Borrelia garinii was the most prevalent genospecies (61%), followed by B. valaisiana (24%), B. afzelii (9%), B. turdi (5%) and B. lusitaniae (0.5%). A novel Borrelia genospecies “Candidatus Borrelia aligera” was also detected. Multilocus sequence typing (MLST) analysis of B. garinii isolates together with the global collection of B. garinii genotypes obtained from the Borrelia MLST public database revealed that: (a) there was little overlap among genotypes from different continents, (b) there was no geographical structuring within Europe, and (c) there was no evident association pattern detectable among B. garinii genotypes from ticks feeding on birds, questing ticks or human isolates. These findings strengthen the hypothesis that the population structure and evolutionary biology of tick‐borne pathogens are shaped by their host associations and the movement patterns of these hosts.

show abstract

Section: Molecular Analysismentioning

confidence: 96%

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Norte

Margos²,

Becker

et al. 2020

Molecular Ecology

View full text Add to dashboard Cite

show abstract

“…In total 17,893 ticks were collected and further processed for TBEV analysis. Regarding the tick species, 99.46% of all collected ticks were from the genus Ixodes and all were identified as Ixodes ricinus, although no further efforts were made to separate into Ixodes inopinatus that has been recently described also outside the Mediterranean region and may also be present in the OWH [45]. Eighty-four ticks (0.54%) were identified as Dermacentor reticulatus.…”

Section: Systematic Tick Field Collection Using the Public As Multiplmentioning

confidence: 99%

Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany

et al. 2020

View full text Add to dashboard Cite

Background: Tick-borne encephalitis (TBE) is the most common viral CNS infection with incidences much higher than all other virus infections together in many risk areas of central and eastern Europe. The Odenwald Hill region (OWH) in southwestern Germany is classified as a TBE risk region and frequent case numbers but also more severe infections have been reported within the past decade. The objective of the present study was to survey the prevalence of tick-borne encephalitis virus (TBEV) in Ixodes ricinus and to associate TBEV genetic findings with TBE infections in the OWH. Methods: Ticks were collected by the flagging methods supported by a crowdsourcing project implementing the interested public as collectors to cover completely and collect randomly a 3532 km 2 area of the OWH TBE risk region. Prevalence of TBEV in I. ricinus was analysed by reversed transcription quantitative real-time PCR. Phylogeographic analysis was performed to classify OWH TBEV isolates within a European network of known TBEV strains. Mutational sequence analysis including 3D modelling of envelope protein pE was performed and based on a clinical database, a spatial association of TBE case frequency and severity was undertaken. Results: Using the crowd sourcing approach we could analyse a total of 17,893 ticks. The prevalence of TBEV in I. ricinus in the OWH varied, depending on analysed districts from 0.12% to 0% (mean 0.04%). Calculated minimum infection rate (MIR) was one decimal power higher. All TBEV isolates belonged to the European subtype. Sequence analysis revealed a discontinuous segregation pattern of OWH isolates with two putative different lineages and a spatial association of two isolates with increased TBE case numbers as well as exceptional severe to fatal infection courses.

show abstract

“…( 2014 ) and Chitimia-Dobler et al. ( 2018 ) were used. To compare the results of this study with others published before 2014, these two species were combined under the term I. ricinus species complex.…”

Section: Methodsmentioning

confidence: 99%

“… 2014 ; Chitimia-Dobler et al. 2018 ). Although ticks and the occurrence of tick-borne pathogens have been investigated in numerous studies in Vienna (Blaschitz et al.…”

Section: Introductionmentioning

confidence: 99%

“…In the urban and suburban areas of Vienna, Ixodes ricinus is the most common tick species, followed by Dermacentor reticulatus and Haemaphysalis concinna, with I. ricinus being the most important vector of pathogens (Radda et al 1986). The recently described species Ixodes inopinatus could also be a vector of pathogens (Estrada-Peña et al 2014;Chitimia-Dobler et al 2018). Although ticks and the occurrence of tick-borne pathogens have been investigated in numerous studies in Vienna (Blaschitz et al 2008;Leschnik et al 2012;Wijnveld et al 2016;Schötta et al 2017;Weiler et al 2017), a long-term monitoring of the questing tick density is still lacking.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Long-term monitoring of the seasonal density of questing ixodid ticks in Vienna (Austria): setup and first results

Vogelgesang

Walter

Kahl³

et al. 2020

Exp Appl Acarol

Self Cite

View full text Add to dashboard Cite

The first long-term monitoring to document both activity and density of questing ixodid ticks in Vienna, Austria, is introduced. It was started in 2017 and is planned to run over decades. Such long-term monitorings are needed to quantify possible effects of climate change or to develop tick density forecast models. The monthly questing tick density at three sites has been observed by using a standardized sampling method by dragging an area of 100 m 2 at each occasion. Popular recreational areas were chosen as study sites. These are the Prater public park, the wooded Kahlenberg, and a wildlife garden in Klosterneuburg. First results show a 3-year time series of nymphs and adults of the Ixodes ricinus species complex and Haemaphysalis concinna for the period 2017-2019. Whereas questing nymphs of the I. ricinus species complex were collected from February to November, H. concinna nymphs were only dragged from May to October. The peak of nymphal activity of the I. ricinus species complex was in May, that of H. concinna in August. In addition, a brief overview is given about ticks and tick-borne pathogens occurring in urban and suburban areas of Vienna.

show abstract

Ixodes inopinatus − Occurring also outside the Mediterranean region

Cited by 71 publications

References 13 publications

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany

Long-term monitoring of the seasonal density of questing ixodid ticks in Vienna (Austria): setup and first results

Contact Info

Product

Resources

About