J. M. G. LeClezio

Cottrell, Robert D.; Waelti-Walters, Jennifer

doi:10.2307/324383

Cited by 2 publications

(3 citation statements)

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“…Similar results have recently been reported for the actions of alphaxalone on GABAA receptors recorded from voltage-clamped rodent central neurones Cottrell et al, 1986b). Alphaxalone does not influence glycineevoked depolarizations in a slice preparation of the rat cuneate nucleus (Harrison & Simmonds, 1984), and far greater concentrations of alphaxalone are required to block ACh-induced currents and voltage-activated sodium currents than those required for modulation of the GABAA receptor (Cottrell et al, 1986a). Collectively, these observations suggest that alphaxalone is a selective modulator of the GABAA receptor.…”

Section: Discussionsupporting

confidence: 86%

“…Bovine chromaffin cells were chosen because they have been shown to possess GABAA receptors, which are similar to those found on central neurones (Bormann & Clapham, 1985;Cottrell et al, 1985), and chemically-activated membrane currents may readily be recorded using the 'whole-cell' mode of the patch-clamp technique (Hamill et al, 1981;Marty & Neher, 1983). A preliminary account ofa part ofthis work has appeared in abstract form (Cottrell et al, 1986a).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of GABA_A receptor activity by alphaxalone

Cottrell

Lambert

Peters

1987

British J Pharmacology

199

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1 The modulation of the y-aminobutyric acidA (GABAA) receptor by alphaxalone has been investigated by use of voltage-clamp recordings from enzymatically isolated bovine chromaffin cells maintained in cell culture. 2 Alphaxalone ( > 30 nM) reversibly and dose-dependently potentiated the amplitude of membrane currents elicited by locally applied GABA (100 ptM). The potentiation was not associated with a change in the reversal potential of GABA-evoked currents and was not influenced by the benzodiazepine receptor antagonist, Rol 5-1788 (300 nM). 3 At relatively high concentrations (>1 JM), alphaxalone directly elicited a membrane current. It is concluded that such currents result from GABAA receptor activation since they were reversibly suppressed by bicuculline (3 AM), dose-dependently enhanced by phenobarbitone (100-500 !LM), and had a similar reversal potential ( -0 mV) to currents elicited by GABA. Additionally, on outside-out membrane patches, alphaxalone activated single channel currents with amplitudes and a reversal potential similar to those evoked by GABA.4 Alphaxalone (30 nM-1 tiM) had no effect upon the amplitude of membrane currents elicited by locally applied acetylcholine (ACh) (100 pM). However, higher concentrations of alphaxalone (10-100 JM) reversibly suppressed ACh-evoked currents, the IC50 for blockade being 20 JAM.5 The P-hydroxy isomer of alphaxalone, betaxalone (100 nM-l pM), did not potentiate GABAinduced currents, nor did higher concentrations of the steroid (10-100 pM) directly evoke a membrane current. However, over the latter concentration range, betaxalone suppressed the amplitude of currents elicited either by GABA or ACh. 6 The relevance of the present results to the anaesthetic action of alphaxalone is discussed together with the broader implications of steroidal modulation of the GABAA receptor.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Modulation of GABA_A receptor activity by alphaxalone

Cottrell

Lambert

Peters

1987

British J Pharmacology

199

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“…Of these galaxies M82 and N520 are both interacting and have compact radio nuclei (Stocke et al 1978). They all may be interacting (Cottrell 1978). M82 and probably all of these galaxies are undergoing intense bursts of star formation (e.g.…”

Section: Irregular Galaxies (Lmc-type)mentioning

confidence: 99%

Star Formation in the Nuclei of Normal Galaxies

Biermann

1980

Highlights astron.

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For the purpose of this talk, I define all those galaxies as normal whose nuclear regions are not known to be dominated by a compact source. Thus I will exclude N galaxies, Seyfert galaxies and radio galaxies, but include galaxies like M81 and M82 which have weak compact nuclei. My review is organized in three parts, (i) tracers of star formation, (ii) star formation in the nuclear regions of observed galaxies, and (iii) the theoretical attempts to interpret the observational material.

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J. M. G. LeClezio

Cited by 2 publications

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Modulation of GABA_A receptor activity by alphaxalone

Modulation of GABA_A receptor activity by alphaxalone

Star Formation in the Nuclei of Normal Galaxies

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J. M. G. LeClezio

Cited by 2 publications

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Modulation of GABAA receptor activity by alphaxalone

Modulation of GABAA receptor activity by alphaxalone

Star Formation in the Nuclei of Normal Galaxies

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Modulation of GABA_A receptor activity by alphaxalone

Modulation of GABA_A receptor activity by alphaxalone