Jagged1 is Clinically Prognostic and Promotes Invasion of Glioma-Initiating Cells by Activating NF-κB(p65) Signaling

Long, Hai; Liu, Peidong; Yu, Shukun; Li, Yi; Tao, Zhennan; Zhang, Chen; Abeysekera, Iruni Roshanie; Li, Tao; Tong, Luqing; Ma, Haiwen; Liu, Bo; Xie, Yang; Zhou, Xuedong; Lin, Yu; Zhu, Meng; Zhang, Kai; Ren, Biao; Ming, Haolang; Huang, Yubao; Yang, Xuejun

doi:10.1159/000496044

Cited by 17 publications

(8 citation statements)

References 43 publications

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“…Notch signaling mediates direct cell-cell interactions and plays a crucial part in cell fate maintenance and selfrenewal of GICs (107). Moreover, studies have shown that down-regulation of Jagged1 induces apoptosis and inhibits proliferation in glioma cell lines (108). Similarly, different variants of OCT4 (POU5F1) have been related to colony formation and regulation of cell survival in GSC (109).…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

Aslam

Abusharieh

Abuarqoub

et al. 2020

Preprint

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Abstract Background. Cancer stem cells (CSCs) use their stemness properties such as self renewal, toxicity, plasticity, and communication with the tumor microenvironment (TME) to perpetuate their lineage and survive chemotherapy. Learning how to interrupt the self renewal ability or modulate the interaction of CSCs with the TME signaling will dramatically improve therapeutic impact on patient’s remission. Anti-tumor properties of mesenchymal stem cells (MSCs) are currently under investigations and different approaches have been applied to gain beneficial effects However, different types of MSCs yielded different conflicting results. In order to investigate if different types of MSCs preconditioned in the same culture conditions can exert alike anti oncogenic effect on glioma stem cells, we planned this study. Methods. GSCs were isolated from U87 cell line by FACS cell sorter, characterized and established as gliospheres. Condition media from MSCs of Wharton Jelly (WJ-MSCs) and bone marrow (BM-MSCs) were harvested and used as treatments on glioshperes (3D) to investigate the effect on proliferation, invasion and self renewal properties of GSCs. Microarray analysis was used to determine the effect at molecular level. Specific human CSC gene arrays were applied to validate the findings of the microarray explicitly the pluripotency of the GSCs. Results. Our results from functional and molecular assays showed that condition media (CM) from both types of MSCs inhibited the metabolism by interrupting oxidative phosphorylation, arrested the cell cycle, induced cell differentiation, targeted the pluripotency and up-regulated the immune response in GSCs. Moreover , condition media from both types of MSCs significantly affected the same genes (KITLG and DKK1) causing a similar effect while using slightly different routes and signaling pathways signifying their individual effects.Conclusion.We conclude that mesenchymal stem cells possess antitumor properties and paracrine factors of mesenchymal stem cells in combination with anti-immune modalities can provide novel therapeutic targets for glioma treatment.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

Aslam

Abusharieh

Abuarqoub

et al. 2020

Preprint

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“…Furthermore, by screening for possible signaling pathways using a dual luciferase reporter assay (Figure S6), we found that the LKB1 gene regulated NF-κB signaling, which plays important roles in cancer growth and metastasis. P65 is a regulatory transcription factor particularly vital in cell survival, growth, and differentiation 21,22. NF-κB activation blocked Snail degradation, subsequently promoting cancer cell migration and invasion 23,24.…”

Section: Discussionmentioning

confidence: 99%

<p>LKB1 suppresses glioma cell invasion via NF-κB/Snail signaling repression</p>

Yuan

Cao

et al. 2019

OTT

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Background: Liver kinase B1 (LKB1) is involved in various human diseases. Aberrant expression of LKB1 expression is involved in glioma progression and associated with prognosis, however, the specific mechanism involving NF-κB/Snail signaling pathways remain unknown. Materials and methods: In the present study, quantitative real-time PCR analysis was used to investigate the expression of LKB1 tumor tissue samples and cell lines. In glioma cell lines, CCK-8 assay, transwell invasion and migration assays were used to investigate the effects of LKB1on proliferation and invasion. Results: We observed that LKB1 knockdown promoted glioma cell proliferation, migration and invasion. This effect was induced through NF-κB/Snail signaling activation. Also, LKB1 overexpression suppressed proliferation, migration, and invasion, which could be rescued by Snail overexpression. Conclusion: Taken together, our results show that LKB1 knockdown promotes remarkably glioma cell proliferation, migration and invasion by regulating Snail protein expression through activating the NF-κB signaling. This may serve as a potential prognostic marker and therapeutic target for glioma.

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“…Hepatoblastoma (HB), a malignant embryonic tumor, is a common liver tumor in children. Liu’s study used gene sequencing to detect the differential expression of circRNA in the HB tissue and adjacent tissue [ 32 ]. They found a substantially upregulated expression of circ_0015756 in HB tissues.…”

Section: Circrna and Common Smts In Childrenmentioning

confidence: 99%

The emerging role of circular RNAs in common solid malignant tumors in children

Yang

2021

Cancer Cell Int

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Malignant tumors are one of the fatal diseases that threaten children’s physical and mental health and affect their development. Research has shown that the occurrence and development of malignant tumors are associated with the abnormal expression and regulation of genes. Circular RNAs (circRNAs) are noncoding RNAs that have a closed circular structure, with a relatively stable expression, and do not undergo exonuclease-mediated degradation readily. Recent studies have shown that circRNA plays an important role in the occurrence, metastasis, and invasion of solid malignant tumors (SMTs) in children. Thus, circRNA is being considered as a breakthrough in the treatment of SMTs in children. In this review, we describe the functions and mechanisms of circRNAs involved in SMTs in children oncogenesis, and summarize the roles of circRNAs in regulating cell proliferation, cell apoptotic death, the cell cycle, cell migrative and invasive ability, epithelial-mesenchymal transition (EMT), cancer stem cells and drug resistance in SMTs in children. In addition, we also discuss the role of circRNAs in the early diagnosis, pathological grading, targeted therapy, and prognosis evaluation of common SMTs in children. CircRNAs are likely to provide a novel direction in therapy in SMTs of children.

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Jagged1 is Clinically Prognostic and Promotes Invasion of Glioma-Initiating Cells by Activating NF-κB(p65) Signaling

Cited by 17 publications

References 43 publications

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

<p>LKB1 suppresses glioma cell invasion via NF-κB/Snail signaling repression</p>

The emerging role of circular RNAs in common solid malignant tumors in children

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Jagged1 is Clinically Prognostic and Promotes Invasion of Glioma-Initiating Cells by Activating NF-κB(p65) Signaling

Cited by 17 publications

References 43 publications

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

WITHDRAWN: Anti-Oncogenic Activities Exhibited By Secretomes Of Mesenchymal Stem Cells Are Mediated By Modulation Of KITLG and DKK1 Genes In Glioma Stem Cells.

<p>LKB1 suppresses glioma cell invasion via NF-&kappa;B/Snail signaling repression</p>

The emerging role of circular RNAs in common solid malignant tumors in children

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<p>LKB1 suppresses glioma cell invasion via NF-κB/Snail signaling repression</p>