JARID1B Protein Expression and Prognostic Implications in Uveal Melanoma

Radberger, Patrik; Radberger, Agatha; Bykov, Vladimir J.N.; Seregard, Stefan; Economou, Mario A.

doi:10.1167/iovs.11-9296

Cited by 17 publications

(12 citation statements)

References 47 publications

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“…Compared with melanoma cells that do not express this enzyme [90], JAR-ID1B + cells cycled more slowly but also generated more progeny and were more tumorigenic [91]. The same result was found for uveal melanoma cells [92]. However, the presence of a subset of cells functionally enriched for tumorigenicity in melanoma is in contrast with the findings obtained by Morrison and coworkers.…”

Section: Activity Of Intracellular Enzymessupporting

confidence: 62%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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Cancer stem cells (CSCs) constitute a subpopulation of cancer cells that have the potential for self-renewal, multipotent differentiation, and tumorigenicity. Studies on CSC biology and CSC-targeted therapies depend on CSC isolation and/or enrichment methodologies. Scientists have conducted extensive research in this field since John Dick's group successfully isolated CSCs based on the expression of the CD34 and CD38 surface markers. Progress in CSC research has been greatly facilitated by the enrichment and isolation of these cells. In this review, we summarize the current strategies used in our and other laboratories for CSC isolation and enrichment, including methods based on stem cell surface markers, intracellular enzyme activity, the concentration of reactive oxygen species, the mitochondrial membrane potential, promoter-driven fluorescent protein expression, autofluorescence, suspension/adherent culture, cell division, the identification of side population cells, resistance to cytotoxic compounds or hypoxia, invasiveness/adhesion, immunoselection, and physical property. Although many challenges remain to be overcome, it is reasonable to believe that more reliable, efficient, and convenient methods will be developed in the near future.

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Section: Activity Of Intracellular Enzymessupporting

confidence: 62%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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“…Roesch et al [3] used especially prepared antibodies that are not available on the market. The antibodies used in our study were similar to those used by Radberger et al [5], who detected the expression of JARID1B in 55% of uveal melanomas investigated. According to Roesch et al [3], their polyclonal primary antibodies were specific to the protein fragment comprising 237-273aa, specific for the RBP2-H1 splicing variant of JARID1B.…”

Section: Discussionmentioning

confidence: 70%

“…Finally, although the limited number of samples does not allow a definite conclusion, the similar expression levels of JARID1B found for the correlated pairs of the primary and recurrent and metastatic melanomas suggest that similar to uveal melanoma [5], the protein cannot be considered a prognostic marker for cutaneous melanoma. However, further studies involving a greater number of cases are necessary for proper evaluation of the prognostic value of JARID1B for human melanoma.…”

Section: Discussionmentioning

confidence: 88%

JARID1B expression in human melanoma and benign melanocytic skin lesions

Kuźbicki

Lange²,

Strączyńska-Niemiec³

et al. 2013

Melanoma Research

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It has been suggested that dynamically regulated expression of the JARID1B protein is required for the continuous growth of tumors and at the same time downregulated in melanoma. The majority of the data on a role of JARID1B in maintaining tumor growth has come from in-vitro and xenografting experiments, with only one immunohistochemical study involving human tissues. We compared JARID1B expression levels in human melanomas and benign nevi and analyzed patterns of spatial distributions of positive cells among different skin layers of the lesions. The expression of JARID1B was evaluated by immunohistochemistry in formalin-fixed paraffin-embedded samples of 30 nevi, 27 primary melanomas, four lymph node metastases, and one local recurrence of melanoma. Staining for JARID1B protein was stronger in melanomas compared with nevi. We also found a significant difference in the spatial distribution of positive cells in individual skin layers of nevi and melanomas. Staining of melanocytes located in granular and spinous layers of nevi was observed very rarely, whereas for melanomas, the mean percentage fractions of positive cells present in these layers exceeded the maximum values found for nevi. The spatial patterns and expression levels of JARID1B did not change significantly with melanoma progression and were similar for primary, metastatic, and recurrent melanomas. Contrary to earlier reports, this study shows enhanced expression of JARID1B by melanoma cells and indicates that such an enhancement may be an early event in the disease progression, is not correlated with melanoma invasiveness, and therefore may not be a suitable candidate as a prognostic marker.

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“…Thus, the JARID1B/KDM5B protein could be recruited to DNA directly through the ARID DNA-binding domain, or through other transcription factors (Barrett et al, 2007). JARID1B/KDM5B specifically removes methyl groups from tri-, di-, and mono-methylated lysine 4 residues on histone H3, facilitating access to DNA by RNA polymerase II (Radberger et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical detection and clinicopathological significance of JARID1B/KDM5B and P16 expression in invasive ductal carcinoma of the breast

Zhao¹,

Liu²

2015

Genet. Mol. Res.

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ABSTRACT. The aims of this study were to investigate the expression of the H3K4 demethylase, jumonji AT-rich interactive domain 1B (JARID1B/KDM5B) and of p16 (multiple tumor suppressor gene MTS1) in breast cancer tissue and determine its clinicopathological significance. JARID1B/KDM5B and P16 protein expression in 176 resected breast cancer specimens and adjacent normal breast tissue was detected by the streptavidin-peroxidase (S-P) immunohistochemical method. The TNM staging grade was assigned according to the World Health Organization (2012) breast classification system. The positive staining rate of JARID1B/KDM5B and p16 protein in cancer tissue was 74.43 and 35.8%, respectively. JARID1B/KDM5B protein expression was positively associated with T grade, Bloom and Richardson (B&R) score and axillary lymph node metastasis (P < 0.05). p16 protein expression was negatively associated with T grade, B&R score, and axillary lymph node metastasis (P < 0.05). JARID1B/KDM5B and p16 protein expression in breast cancer and adjacent normal breast tissue were negatively correlated (r = -0.303, P < 0.001). The data demonstrated 5418 L.H.

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JARID1B Protein Expression and Prognostic Implications in Uveal Melanoma

Cited by 17 publications

References 47 publications

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

JARID1B expression in human melanoma and benign melanocytic skin lesions

Immunohistochemical detection and clinicopathological significance of JARID1B/KDM5B and P16 expression in invasive ductal carcinoma of the breast

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