Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings

Abstract

Protein pattern, ammonia content, glutamine synthetase activity, lipid peroxidation, superoxide dismutase, catalase, peroxidase and peroxidase isoforms were studied in the leaves and roots of 7-d-old peanut (Arachis hypogaea L. cv. JL-24) seedlings treated by 25, 100 and 250 μM jasmonic acid (JA). SDS-PAGE protein profile of leaves and roots after JA application showed a significant increase in 18, 21, 30, 45, 47 and 97.4 kDa proteins and significant decrease in 22 and 36 kDa proteins. Pathogenesis related PR-18 was specific in leaves at 250 μM JA and PR-21 have cross reacted differently with 21 and 30 kDa proteins in leaves and roots treated by all JA concentrations. Further, the immunoblot analysis with glutamine synthetase, GS-45 antibodies revealed a specific cross reaction with 45 and 47 kDa proteins of both control and JA treated leaves, however, higher at 100 and 250 μM JA treated leaves than control ones. Further, the malondialdehyde (MDA) content significantly increased in leaves and roots treated with JA, indicated membrane damage with JA treatments that led to the generation of peroxidation products. The peroxidase isozymic pattern showed two specific isoforms. Besides, the activities of SOD and catalase were significantly elevated in JA treated leaves.

Effect of Jasmonic Acid on Photosynthetic Pigments and Stress Markers in <i>Cajanus cajan</i> (L.) Millsp. Seedlings under Copper Stress

“…1 nM JA treatment resulted in about 2% decrease in MDA content but in other JA (1 µM and 1 pM) treatments 0.8% to 1.86% increase in MDA content was noticed. Enhancement of MDA content subjected to 24, 100 and 250 µM JA was noticed in peanut seedlings [21] and in Scenedesmus incrassatulus [22]. Drought stress increased membrane leakage in Ocimum basilicum L. [14].…”

“…JA reported to induce accumulation of a specific set of proteins, the so called jasmonate induced stress proteins (JISP) in rice seedlings [30]. JA induced changes in protein pattern in peanut seedlings in very dose dependent and specificity manner [21].…”

Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves

“…Catalase isozymes differ in biochemical properties, as well as in their developmental specificity (occasionally related to germination), but their principal function probably involves fatty acid conversion, whilst other isozymes are related to lignification, photorespiration or the aging process (Kumari et al, 2006). The peroxidases (Prx) are associated with biochemical and physiological processes such as growth, cell formation, fruit development, ethylene biosynthesis, as well as the response to various stresses (Matamoros et al, 2003).…”

Mechanisms of Hormone Regulation for Drought Tolerance in Plants

“…Similar to the current state of SA research, jasmonates have been implicated in promoting tolerance to abiotic stresses including salinity, ozone, or UV-B irradiance through downstream effects on antioxidant metabolism, whereas less research has been conducted regarding the contribution of jasmonates to antioxidant-facilitated drought tolerance (Kumari et al 2006 ). Nevertheless, several studies which have been conducted suggest jasmonates mitigate the drought-induced oxidative burst in a similar manner as for other oxidative stresses, by means of increased antioxidant enzyme activities.…”