Jejunal epithelial barrier disruption triggered by reactive oxygen species in early SIV infected rhesus macaques

Wang, Xuehui; Song, Tian-Zhang; Zheng, Hong-Yi; Li, Yihui; Zheng, Yong‐Tang

doi:10.1016/j.freeradbiomed.2021.10.026

Cited by 9 publications

(5 citation statements)

References 71 publications

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“…Briefly, surface-labeled platelets incubated with 2 μM fluorescent probe for 15 min at 37 °C. Mitochondrial membrane potential was measured using the fluorescent dye Rh123 (R-8004, China) performed as previously described [ 30 ]. After labeled for the surface markers CD41, platelets were incubated with Rh123 at a final concentration of 2.5 μM for 15 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway

Wang

Huang

et al. 2023

Redox Biology

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Section: Methodsmentioning

confidence: 99%

RETRACTED: Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway

Wang

Huang

et al. 2023

Redox Biology

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“…In addition, CVM and its metabolites directly influence the epithelial barrier by inducing oxidative stress, altering miRNA expression, and promoting cell cycle arrest and apoptosis. BVassociated bacteria induce oxygen stress intermediates when cocultured with a 3D cervical epithelial model which affects the epithelial barrier integrity (Wang et al, 2021b). P. bivia and S. amnii increase 2-hydroxyglutarate, 2-hydroxybutyrate, and citrulline levels, which are correlated to the activity of inducible nitric oxide synthase (iNOS) (Łaniewski and Herbst-Kralovetz, 2021).…”

Section: The Effect Of Cervicovaginal Dysbiosis On Epithelial Barrier...mentioning

confidence: 99%

Interactions between microbiota and cervical epithelial, immune, and mucus barrier

Dong

Bai

et al. 2023

Front. Cell. Infect. Microbiol.

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The female reproductive tract harbours hundreds of bacterial species and produces numerous metabolites. The uterine cervix is located between the upper and lower parts of the female genital tract. It allows sperm and birth passage and hinders the upward movement of microorganisms into a relatively sterile uterus. It is also the predicted site for sexually transmitted infection (STI), such as Chlamydia, human papilloma virus (HPV), and human immunodeficiency virus (HIV). The healthy cervicovaginal microbiota maintains cervical epithelial barrier integrity and modulates the mucosal immune system. Perturbations of the microbiota composition accompany changes in microbial metabolites that induce local inflammation, damage the cervical epithelial and immune barrier, and increase susceptibility to STI infection and relative disease progression. This review examined the intimate interactions between the cervicovaginal microbiota, relative metabolites, and the cervical epithelial-, immune-, and mucus barrier, and the potent effect of the host-microbiota interaction on specific STI infection. An improved understanding of cervicovaginal microbiota regulation on cervical microenvironment homeostasis might promote advances in diagnostic and therapeutic approaches for various STI diseases.

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“…Peripheral blood and bone marrow derived plasma virus RNA was extracted using the High Pure Viral RNA Kit (Roche, Germany) in accordance with the manufacturer’s protocols. Tissue RNA was extracted using RNAisoPlus Reagent (Takara, Japan) as previously described ( 21 ). For detection of SFTSV RNA, a THUNDERBIRD Probe One-Step qRT-PCR Kit (TOYOBO, Japan) was used in accordance with the manufacturer’s protocols.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal analysis of immunocyte responses and inflammatory cytokine profiles in SFTSV-infected rhesus macaques

Huang

et al. 2023

Front. Immunol.

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Severe fever with thrombocytopenia syndrome virus (SFTSV), an emerging bunyavirus, causes severe fever with thrombocytopenia syndrome (SFTS), with a high fatality rate of 20%–30%. At present, however, the pathogenesis of SFTSV remains largely unclear and no specific therapeutics or vaccines against its infection are currently available. Therefore, animal models that can faithfully recapitulate human disease are important to help understand and treat SFTSV infection. Here, we infected seven Chinese rhesus macaques (Macaca mulatta) with SFTSV. Virological and immunological changes were monitored over 28 days post-infection. Results showed that mild symptoms appeared in the macaques, including slight fever, thrombocytopenia, leukocytopenia, increased aspartate aminotransferase (AST) and creatine kinase (CK) in the blood. Viral replication was persistently detectable in lymphoid tissues and bone marrow even after viremia disappeared. Immunocyte detection showed that the number of T cells (mainly CD8+ T cells), B cells, natural killer (NK) cells, and monocytes decreased during infection. In detail, effector memory CD8+ T cells declined but showed increased activation, while both the number and activation of effector memory CD4+ T cells increased significantly. Furthermore, activated memory B cells decreased, while CD80+/CD86+ B cells and resting memory B cells (CD27+CD21+) increased significantly. Intermediate monocytes (CD14+CD16+) increased, while myeloid dendritic cells (mDCs) rather than plasmacytoid dendritic cells (pDCs) markedly declined during early infection. Cytokines, including interleukin-6 (IL-6), interferon-inducible protein-10 (IP-10), and macrophage inflammatory protein 1 (MCP-1), were substantially elevated in blood and were correlated with activated CD4+ T cells, B cells, CD16+CD56+ NK cells, CD14+CD16+ monocytes during infection. Thus, this study demonstrates that Chinese rhesus macaques infected with SFTSV resemble mild clinical symptoms of human SFTS and provides detailed virological and immunological parameters in macaques for understanding the pathogenesis of SFTSV infection.

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Jejunal epithelial barrier disruption triggered by reactive oxygen species in early SIV infected rhesus macaques

Cited by 9 publications

References 71 publications

RETRACTED: Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway

RETRACTED: Severe fever with thrombocytopenia syndrome virus induces platelet activation and apoptosis via a reactive oxygen species-dependent pathway

Interactions between microbiota and cervical epithelial, immune, and mucus barrier

Longitudinal analysis of immunocyte responses and inflammatory cytokine profiles in SFTSV-infected rhesus macaques

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