Joint Report from a Mixed Lymphocyte Culture Workshop

Thorsby, E.; Bois, Ria Du; Bondevik, H; Dupont, Bo; Eijsvoogel, V. P.; Hansen, John A.; Jersild, Casper; Jørgensen, F.; Kissmeyer‐Nielsen, F.; Lamm, L. U.; Schellekens, P. T. A.; Svejgaard, A.; Thomsen, Mögens

doi:10.1111/j.1399-0039.1974.tb00282.x

Cited by 31 publications

(8 citation statements)

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“…This "typing response" makes clear-cut LD-1 determinant typing difficult. Expression of the results as a relative response seems to give the greatest stability, as has also been previously demonstrated (see Lamm & Svejgaard in Thorsby et al 1974). However, repeated investigations, or the use of several stimulating cells homozygous for the same LD-I locus alleles, are most often necessary for a reliable definition of LD-1 determinants.…”

Section: Discussionsupporting

confidence: 75%

Identification of Five Lymphocyte Activating Determinants in Man

et al. 1975

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Eleven putative LD‐1 locus homozygous cell donors were found by MLC tests in non‐con‐sanguineous families. Lymphocytes from each of them were used as stimulating typing cells in MLC! tests together with responding cells from the other homozygous cell donors and from 45 random unrelated individuals. Four of the stimulating typing cells were found to identify a determinant in non‐random association with IIL‐.A7; LD‐ 7a, three other cells typed for a determinant non‐randomly associated with HL‐A8; LD‐8a, and one cell seems to identify LD‐W5 in non‐random association with W5. Two possible additional LD‐1 determinants, LD‐oh and LD‐pm, were less well defined with two of the cells. The combined gene frequency of the five possible LD‐1 determinants is approximately 0.45 among Norwegians.

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Section: Discussionsupporting

confidence: 75%

Identification of Five Lymphocyte Activating Determinants in Man

et al. 1975

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“…As a whole, we have found the test to be reliable in serving its purpose. The Workshop cooperation with three other laboratories has, however, induced some minor adjustments (see Joint Report, Thorsby et al 1974).…”

Section: Discussionmentioning

confidence: 99%

A Mixed Lymphocyte Culture Micro‐Technique and its Application in Different Family and Unrelated Combinations

et al. 1974

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An MLC micro‐test is described, using micro‐plates with 0.15 × 106 responding cells and the same number of stimulating cells per well, in a volume of 0.2 ml. Studies on technical variation and biological reproducibility are reported, as well as the application of the test in related and unrelated combinations. The method was found to be reliable and to have satisfactory discriminative power in different allogeneic combinations. Furthermore, our technique could be used for “LD‐1 locus typing”.

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“…When allogenic human lymphocytes are cultured in vitro in mixed lymphocyte cultures (MLC), a strong proliferative response occurs if the lymphocytes differ for HLA-D determinants (Thorsby & Piazza 1975). These determinants have previously been shown to be expressed on lymphocytes, mainly B lymphocytes (Lohrmann e t al.…”

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confidence: 99%

Stimulation of Human Lymphocytes by Allogenic Macrophages in vitro

Kaakinen¹,

Hirschberg

1977

Tissue Antigens

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Macrophages were obtained from human peripheral blood by incubating mononuclear cells in plastic tissue culture flasks. After 1—14 days, the cells were used in mixed lymphocyte macrophage cultures. Macrophages could not, themselves, be stimulated to proliferation by allogenic cells, but stimulated allogenic lymphocytes. By the use of responding cells and stimulating macrophages from HLA‐D homozygous individuals, the HLA‐D determinants could be shown to be responsible for the stimulation of allogenic lymphocytes.

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Joint Report from a Mixed Lymphocyte Culture Workshop

Cited by 31 publications

References 4 publications

Identification of Five Lymphocyte Activating Determinants in Man

Identification of Five Lymphocyte Activating Determinants in Man

A Mixed Lymphocyte Culture Micro‐Technique and its Application in Different Family and Unrelated Combinations

Stimulation of Human Lymphocytes by Allogenic Macrophages in vitro

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