Justification of an alternative method for determination of toxicity of perfume and beauty products by in vitro method on a short-term suspension cell culture-cattle spermatozoa (literature review)

Abstract

Introduction. Based on the literature data and directive documentation, the main reasons for the use of alternative methods for determining the toxic effects of perfume and beauty products are set out. Methods — alternative methods based on toxicity assessment using short-term suspension culture of cells of cattle spermatozoa. The toxicological prognosis of the alternative method according to the results of studies is close to the significance of the toxicological prognosis obtained in the experiment with the use of laboratory animals. Mobility is an integral parameter of the physiological, biochemical and morphological status of the short-term suspension culture of mammal cells cattle spermatozoa. Conclusion. The argument for the implementation of alternative methods for determining the toxic effect of perfume and beauty products (PBPs) is aimed at the humane attitude towards animals, as well as reducing costs and reducing the time spent on the study. Key words: toxicity, perfume and beauty products, alternative methods, spermatozoa, cattle.

Introduction of Alternative Methods of Testing Cosmetic Products in Ukraine: Opportunities and Challenges of Today

“…Через високу ціну тривимірних моделей в багатьох країнах світу проводяться спроби валідувати власні моделі штучної шкіри [9][10] [11].…”