Juvenile hormone biosynthesis in larval and adult stick insects, Carausius morosus

Lorenz, Matthias; Hoffmann, Klaus H.; GÄde, Gerd

doi:10.1016/s0022-1910(98)00143-7

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…The rates of stimulation of JH synthesis by farnesol and FA seem to confirm that farnesol-SDRs are less abundant than JHAMTs; addition of high concentrations of farnesol to CA cultures stimulated JH synthesis only 1.8-fold in the adult stink bug Perillus bioculatus (33), 4-fold in the adult stick insect Carausius morosus (34), and 3-fold in adult and embryos of D. punctata cockroaches (35,36). On the other hand, addition of FA increased the rate of JH synthesis 100-fold by CA of the locust Schistocerca gregaria (37) and 40-fold in CA of A. aegypti females (19).…”

Section: Aasdr-1 Efficiently Oxidizes Farnesol To Farnesalmentioning

confidence: 85%

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

Mayoral

Nouzová

Navare

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

107

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The synthesis of juvenile hormone (JH) is an attractive target for control of insect pests and vectors of disease, but the minute size of the corpora allata (CA), the glands that synthesize JH, has made it difficult to identify important biosynthetic enzymes by classical biochemical approaches. Here, we report identification and characterization of an insect farnesol dehydrogenase (AaSDR-1) that oxidizes farnesol into farnesal, a precursor of JH, in the CA. AaSDR-1 was isolated as an EST in a library of the corpora allata-corpora cardiaca of the mosquito Aedes aegypti. The 245-amino acid protein presents the typical short-chain dehydrogenase (SDR) Rossmann-fold motif for nucleotide binding. This feature, together with other conserved sequence motifs, place AaSDR-1 into the ''classical'' NADP ؉ -dependent cP2 SDR subfamily. The gene is part of a group of highly conserved paralogs that cluster together in the mosquito genome; similar clusters of orthologs were found in other insect species. AaSDR-1 acts as a homodimer and efficiently oxidizes C 10 to C15 isoprenoid and aliphatic alcohols, showing the highest affinity for the conversion of farnesol into farnesal. Farnesol dehydrogenase activity was not detected in the CA of newly emerged mosquitoes but significant activity was detected 24 h later. Real time PCR experiments revealed that AaSDR-1 mRNA levels were very low in the inactive CA of the newly emerged female, but increased >30-fold 24 h later during the peak of JH synthesis. These results suggest that oxidation of farnesol might be a rate-limiting step in JH III synthesis in adult mosquitoes.mosquito ͉ Aedes ͉ farnesal ͉ insect

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Section: Aasdr-1 Efficiently Oxidizes Farnesol To Farnesalmentioning

confidence: 85%

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

Mayoral

Nouzová

Navare

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

107

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The Juvenile Hormones

Goodman

2005

Comprehensive Molecular Insect Science

143

192

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Molecular cloning and characterization of three cDNAs encoding allatostatin-like neurosecretory peptides from Pandalopsis japonica

Salma

Jang

Uddowla

et al. 2012

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Juvenile hormone biosynthesis in larval and adult stick insects, Carausius morosus

Cited by 5 publications

References 39 publications

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

The Juvenile Hormones

Molecular cloning and characterization of three cDNAs encoding allatostatin-like neurosecretory peptides from Pandalopsis japonica

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Juvenile hormone biosynthesis in larval and adult stick insects, Carausius morosus

Cited by 5 publications

References 39 publications

NADP + -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

NADP + -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

The Juvenile Hormones

Molecular cloning and characterization of three cDNAs encoding allatostatin-like neurosecretory peptides from Pandalopsis japonica

Contact Info

Product

Resources

About

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis

NADP ⁺ -dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis