K-series centrifuges

Brantley, J.N.; Willis, D.D.; Breillatt, J.P.; Gibson, Ron; Patrick, Lajarge; Anderson, Norman G.

doi:10.1016/0003-2697(70)90380-5

Cited by 10 publications

(3 citation statements)

References 2 publications

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“…The end result of this work included the design and construction of the K-II large-scale ultracentrifuge ( 7 – 10 ). This device was designed to recover virus in a high state of purity from 100-L batches of crude influenza vaccine in an 8-hour day ( 11 , 12 ) and subsequently was used for the large-scale purification of the hepatitis B (Australia) surface antigen ( 13 , 14 ) from human serum for use as a vaccine and for mass isolation of polyhedral inclusion bodies ( 15 ).…”

Section: Virus Isolation Sequencing and Assemblymentioning

confidence: 99%

Global Screening for Human Viral Pathogens

Anderson

Gerin

Anderson³

2003

Emerg. Infect. Dis.

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We propose a system for continuing surveillance of viral pathogens circulating in large human populations. We base this system on the physical isolation of viruses from large pooled samples of human serum and plasma (e.g., discarded specimens from diagnostic laboratories), followed by shotgun sequencing of the resulting genomes. The technology for concentrating virions from 100-L volumes was developed previously at Oak Ridge National Laboratory, and the means for purifying and concentrating virions from volumes in microliters have been developed recently. At the same time, marine virologists have developed efficient methods for concentrating, amplifying, and sequencing complex viral mixtures obtained from the ocean. Given this existing technology base, we believe an integrated, automated, and contained system for surveillance of the human “virome” can be implemented within 1 to 2 years. Such a system could monitor the levels of known viruses in human populations, rapidly detect outbreaks, and systematically discover novel or variant human viruses.

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Section: Virus Isolation Sequencing and Assemblymentioning

confidence: 99%

Global Screening for Human Viral Pathogens

Anderson

Gerin

Anderson³

2003

Emerg. Infect. Dis.

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“…The discovery of novel and reliable biomarkers for disease diagnosis and classification has consistently been of intense interest for oncological applications, where effective screening and early detection can both guide clinical management and significantly improve overall survival. − Plasma proteome analysis is a promising avenue for identifying potential disease biomarkers due to the wide range of tissue-specific proteins accessible through the circulation and the noninvasive nature of blood collection. , Mass spectrometry (MS) has been the method of choice for unbiased profiling of proteins in complex biological samples like blood . However, the dynamic range of protein concentrations in plasma spans across several orders of magnitude, making it difficult to detect low-abundance proteins .…”

Section: Introductionmentioning

confidence: 99%

timsTOF HT Improves Protein Identification and Quantitative Reproducibility for Deep Unbiased Plasma Protein Biomarker Discovery

Vitko,

Chou,

Nouri Golmaei

et al. 2024

J. Proteome Res.

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Mass spectrometry (MS) is a valuable tool for plasma proteome profiling and disease biomarker discovery. However, wide-ranging plasma protein concentrations, along with technical and biological variabilities, present significant challenges for deep and reproducible protein quantitation. Here, we evaluated the qualitative and quantitative performance of timsTOF HT and timsTOF Pro 2 mass spectrometers for analysis of neat plasma samples (unfractionated) and plasma samples processed using the Proteograph Product Suite (Proteograph) that enables robust deep proteomics sampling prior to mass spectrometry. Samples were evaluated across a wide range of peptide loading masses and liquid chromatography (LC) gradients. We observed up to a 76% increase in total plasma peptide precursors identified and a >2-fold boost in quantifiable plasma peptide precursors (CV < 20%) with timsTOF HT compared to Pro 2. Additionally, approximately 4.5 fold more plasma peptide precursors were detected by both timsTOF HT and timsTOF Pro 2 in the Proteograph analyzed plasma vs neat plasma. In an exploratory analysis of 20 late-stage lung cancer and 20 control plasma samples with the Proteograph, which were expected to exhibit distinct proteomes, an approximate 50% increase in total and statistically significant plasma peptide precursors (q < 0.05) was observed with timsTOF HT compared to Pro 2. Our data demonstrate the superior performance of timsTOF HT for identifying and quantifying differences between biologically diverse samples, allowing for improved disease biomarker discovery in large cohort studies. Moreover, researchers can leverage data sets from this study to optimize their liquid chromatography−mass spectrometry (LC−MS) workflows for plasma protein profiling and biomarker discovery. (ProteomeXchange identifier: PXD047854 and PXD047839).

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“…Breillatt,-J. N. Brantley, and R. F. Gibson, manuscript in preparation) was operated in a K-series centrifuge with a C-type casing (4) equipped for where [ temperature control of the rotor and fluid flow lines ters), pp (6). Sample suspensions were held in 20-liter pres-and mrn sure cans (model XX67-000-05 pressure tanks, Milli-flow strf pore Corp., Bedford, Mass.)…”

mentioning

confidence: 99%

Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

Breillatt¹,

Brantley²,

Mazzone³

et al. 1972

Applied Microbiology

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Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 1013 polyhedral inclusion bodies per day. Continuousflow methods for S-p type purification have been evolved to deal with mass isolation of bioparticles.

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K-series centrifuges

Cited by 10 publications

References 2 publications

Global Screening for Human Viral Pathogens

Global Screening for Human Viral Pathogens

timsTOF HT Improves Protein Identification and Quantitative Reproducibility for Deep Unbiased Plasma Protein Biomarker Discovery

Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

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