K562 cell-derived exosomes suppress the adhesive function of bone marrow mesenchymal stem cells via delivery of miR-711

Jiang, Yulin; Liu, Jing; Li, Shu-Qi; Xu, Ye; Min, Qing‐Hua; Zhong, Qiong-Hui; Sun, Fan; Li, Jing; You, Xia-Hong; Liao, Kaili; Qin, Tingyu; Zhao, Chengliang; Huang, Bo; Wang, Xiaozhong

doi:10.1016/j.bbrc.2019.10.096

Cited by 13 publications

(8 citation statements)

References 24 publications

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“…[ 71 ]. Based on a study about K562 cell, K562 exosomal miR-711 has been credited for suppressed adhesion abilities of BM-MSCs because of the fact that miR-711 is capable of silencing CD44—an adhesion molecule‒expression in BM-MSCs [ 107 ].…”

Section: Contact-independentmentioning

confidence: 99%

The mechanisms of mutual relationship between malignant hematologic cells and mesenchymal stem cells: Does it contradict the nursing role of mesenchymal stem cells?

et al. 2022

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Mesenchymal stem/stromal cells (MSCs) are known as the issue in biology because of some unpredictable characteristics in the different microenvironments especially in their bone marrow niche. MSCs are used in the regenerative medicine because of their unique potentials for trans-differentiation, immunomodulation, and paracrine capacity. But, their pathogenic and pro-survival effects in tumors/cancers including hematologic malignancies are indisputable. MSCs and/or their derivatives might be involved in tumor growth, metastasis and drug resistance in the leukemias. One of important relationship is MSCs and hematologic malignancy-derived cells which affects markedly the outcome of disease. The communication between these two cells may be contact-dependent and/or contact-independent. In this review, we studied the crosstalk between MSCs and malignant hematologic cells which results the final feedback either the progression or suppression of blood cell malignancy. Graphical abstract

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Section: Contact-independentmentioning

confidence: 99%

The mechanisms of mutual relationship between malignant hematologic cells and mesenchymal stem cells: Does it contradict the nursing role of mesenchymal stem cells?

et al. 2022

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“…Besides, blast crisis CML (CML-BC) patients had a much higher miR-320 expression in plasma exosomes, compared to chronic phase CML (CML-CP) patients, indicating its clinical relevance ( Gao et al, 2019 ). The study of Jiang et al (2020) revealed the elevation of miR-711 in exosomes derived from K562 cells compared to parental cells exosomes. K562 cell-derived exosomal miR-711 could be transferred into BMMSCs and weaken the adhesive abilities ( Jiang et al, 2020 ).…”

Section: Role Of Exosomal Mirnas In Leukemiamentioning

confidence: 99%

“…The study of Jiang et al (2020) revealed the elevation of miR-711 in exosomes derived from K562 cells compared to parental cells exosomes. K562 cell-derived exosomal miR-711 could be transferred into BMMSCs and weaken the adhesive abilities ( Jiang et al, 2020 ). miR-15a was shuttled by human BMMSC-exosomes which might be associated with the proliferation of CML cells ( Zhang X. et al, 2020 ).…”

Section: Role Of Exosomal Mirnas In Leukemiamentioning

confidence: 99%

The Landscape of Exosome-Derived Non-Coding RNA in Leukemia

Tang

Sun²,

Chen³

et al. 2022

Front. Pharmacol.

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Leukemia is a group of life-threatening hematological malignancies which is currently incurable and often accompanied by drug resistance or disease relapse. Understanding the pathogenesis of leukemia and finding specific therapeutic targets and biomarkers is of great importance to improve the clinical efficacy of leukemia. Exosome-derived ncRNAs have been demonstrated as critical components of intercellular communication and function as key facilitators in the leukemia biological process. This review outlines the current investigations of exosomal ncRNAs (including miRNA, circRNA, and lncRNA) as important mediators of leukemia and potential therapeutic targets and biomarkers for leukemia treatment. Moreover, we generally analyze the prospects and challenges for exosomal ncRNAs from the aspects of research and clinical application.

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“…All patients provided written informed consent. (17). The experimental workflow is shown in Figure 2A.…”

Section: Patients and Plasma Samplesmentioning

confidence: 99%

Quantitative Proteomic Analysis of Plasma Exosomes to Identify the Candidate Biomarker of Imatinib Resistance in Chronic Myeloid Leukemia Patients

Zhao

Chen

et al. 2021

Front. Oncol.

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BackgroundImatinib (IM), a tyrosine kinase inhibitor (TKI), has markedly improved the survival and life quality of chronic myeloid leukemia (CML) patients. However, the lack of specific biomarkers for IM resistance remains a serious clinical challenge. Recently, growing evidence has suggested that exosome-harbored proteins were involved in tumor drug resistance and could be novel biomarkers for the diagnosis and drug sensitivity prediction of cancer. Therefore, we aimed to investigate the proteomic profile of plasma exosomes derived from CML patients to identify ideal biomarkers for IM resistance.MethodsWe extracted exosomes from pooled plasma samples of 9 imatinib-resistant CML patients and 9 imatinib-sensitive CML patients by ultracentrifugation. Then, we identified the expression levels of exosomal proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS) based label free quantification. Bioinformatics analyses were used to analyze the proteomic data. Finally, the western blot (WB) and parallel reaction monitoring (PRM) analyses were applied to validate the candidate proteins.ResultsA total of 2812 proteins were identified in plasma exosomes from imatinib-resistant and imatinib-sensitive CML patients, including 279 differentially expressed proteins (DEPs) with restricted criteria (fold change≥1.5 or ≤0.667, p<0.05). Compared with imatinib-sensitive CML patients, 151 proteins were up-regulated and 128 proteins were down-regulated. Bioinformatics analyses revealed that the main function of the upregulated proteins was regulation of protein synthesis, while the downregulated proteins were mainly involved in lipid metabolism. The top 20 hub genes were obtained using STRING and Cytoscape, most of which were components of ribosomes. Moreover, we found that RPL13 and RPL14 exhibited exceptional upregulation in imatinib-resistant CML patients, which were further confirmed by PRM and WB.ConclusionProteomic analysis of plasma exosomes provides new ideas and important information for the study of IM resistance in CML. Especially the exosomal proteins (RPL13 and RPL14), which may have great potential as biomarkers of IM resistance.

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K562 cell-derived exosomes suppress the adhesive function of bone marrow mesenchymal stem cells via delivery of miR-711

Cited by 13 publications

References 24 publications

The mechanisms of mutual relationship between malignant hematologic cells and mesenchymal stem cells: Does it contradict the nursing role of mesenchymal stem cells?

The mechanisms of mutual relationship between malignant hematologic cells and mesenchymal stem cells: Does it contradict the nursing role of mesenchymal stem cells?

The Landscape of Exosome-Derived Non-Coding RNA in Leukemia

Quantitative Proteomic Analysis of Plasma Exosomes to Identify the Candidate Biomarker of Imatinib Resistance in Chronic Myeloid Leukemia Patients

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