Kaempferol 7- O - β -D-glucoside isolated from the leaves of Cudrania tricuspidata inhibits LPS-induced expression of pro-inflammatory mediators through inactivation of NF-κB, AP-1, and JAK-STAT in RAW 264.7 macrophages

Lee, Seung-Bin; Shin, Ji‐Sun; Han, Hee-Soo; Lee, Hwi-Ho; Park, Jong Chul; Lee, Kyung‐Tae

doi:10.1016/j.cbi.2018.02.022

Cited by 48 publications

(27 citation statements)

References 52 publications

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“…Similar to previous reports 46,47 , we showed undetectable basal IL-1β levels both in vivo and in vitro. In vivo, PGB led to a significant inhibition of LPS-induced IL-1β secretion.…”

Section: Discussionsupporting

confidence: 92%

Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

Abu-rish

Mansour

et al. 2020

Sci Rep

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is observed in the principal PGB-indicated conditions which share the alteration in IL-6, TNF-α, IL-2 and IL-1β cytokines level. Yet, reports on PGB immunomodulatory and anti-inflammatory effects are a few and focused mainly on in vitro assays, local inflammatory changes within the nervous system, and attenuation of the secretion of only IL-1β and TNF-α cytokines 18-20. In addition, PGB effects on cytokines secretion in immune cells, such as isolated splenocytes and peritoneal macrophages (PMs), and its effect on lymphoid organs, were not examined before. Therefore, there is a need to perform a simultaneous assessment of the effect of PGB on the secretion of the cytokines that were commonly elevated in the aforementioned PGB-indicated conditions (IL-6, TNF-α, IL-1β and IL-2) and to expand the investigation to its effect on lymphoid organs and cytokine secretion in immune cells. As peripheral inflammation and elevated systemic cytokines levels were demonstrated in the above-mentioned PGB-indicated conditions, we investigated in this study the effect of PGB on murine models of peripheral inflammation. In this study, we used for the first time, LPS and ConA-induced murine models of inflammation to examine the effect of PGB on peripheral proinflammatory cytokine secretion (IL-6, TNF-α, IL-1β and IL-2) in vitro and in vivo in BALB/c mice. LPS-model of inflammation has been employed before to study neuroinflammatory conditions, seizure and anxiety disorders in mice 21-25 , while ConA was used to investigate T-cell function in patients with fibromyalgia 26. Additionally, in this study, the effect of PGB on mitogen-induced inflammatory changes in the spleen, as a lymphoid organ, was also examined for the first time. Regarding in vitro investigation, the lack of reports that examined the effect of PGB on the secretion of cytokines in immune cells prompted us to investigate such effects of PGB on basal and mitogen-induced proinflammatory cytokines secretion in splenocytes and peritoneal macrophages (PMs).

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“…Similar to previous reports 46,47 , we showed undetectable basal IL-1β levels both in vivo and in vitro. In vivo, PGB led to a significant inhibition of LPS-induced IL-1β secretion.…”

Section: Discussionsupporting

confidence: 92%

Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

Abu-rish

Mansour

et al. 2020

Sci Rep

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“…In LPS-induced RAW264.7 cells, toll-like receptor 4 (TLR4) recognizes and binds to LPS, promoting the association of TLR4 with the adaptor myeloid differentiation factor 88. Subsequently, progressive inflammatory signaling induces activation of mitogen-activated protein kinases (MAPKs), AP-1, and NF-κB protein kinases, eventually contributing to the inflammatory response [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Pterostilbene and 4′-Methoxyresveratrol Inhibited Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages

et al. 2018

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Pterostilbene (Pte) and 4′-Methoxyresveratrol (4MR) are methylated derivatives of resveratrol. We investigated the anti-inflammatory effect of Pte and 4MR in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. Both Pte and 4MR significantly reduced LPS-induced nitric oxide release by inhibiting the inducible nitric oxide synthase mRNA expression. Moreover, both of them inhibited LPS-induced mRNA expression of inflammatory cytokines including monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6 and IL-1β, and tumor necrosis factor α (TNF-α), and attenuated LPS-induced nuclear factor-κB (NF-κB) activation by decreasing p65 phosphorylation. In addition, 4MR but not Pte inhibited LPS-induced the activator protein (AP)-1 pathway in RAW 264.7 macrophages. Further study suggested that Pte had an inhibitory effect on extracellular regulated protein kinases (ERK) and p38 activation, but not on c-Jun N-terminal kinase (JNK), while 4MR had an inhibitory effect on JNK and p38 activation, but not on ERK. Taken together, our data suggested that Pte induced anti-inflammatory activity by blocking mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways, while 4MR showed anti-inflammatory activity through suppression of MAPK, AP-1, and NF-κB signaling pathways in LPS-treated RAW 264.7 macrophages.

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“…Among of them, kaempferol is one of the bio avonoids which was reported to effectively prevent breast cancer cell growth. [39][40][41] More studies showed that kaempferol could enhance the expression of pro-apoptotic related proteins, such as caspase-9, caspase-3, caspase-7, PARP, Bax, p21, p53, and pATM. [42,43] and decrease the expression of anti-apoptotic proteins of pAKT, Bcl2, cyclins A, B, D1, and E, CDK1, PLK-1, IRS-1, MEK1/2, and cathepsin D. [39,[42][43][44][45][46][47] As a natural methoxylated avonoid, nobiletin is the second key compound and well-studied to potent the anti-angiogenic activity in ER + breast cancer cells via regulating Src/FAK/STAT3-mediated signaling pathway through PXN.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology reveals hub bio-active ingredients and possible mechanisms of a Chinese herbal prescription Ru-kang-yin against breast cancer

Chu¹,

Chen²,

Li³

et al. 2020

Preprint

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Background Ru-kang-yin (RKY), a traditional Chinese herbal prescription, has been clinically used as adjuvant therapy for breast cancer via inhibiting cell invasion, proliferation, and promoting apoptosis. However, its anti-breast cancer bio-active ingredients and possible mechanisms are still unclear. In the present study, the hub bio-active compounds and underlying mechanisms of RKY in treatment of breast cancer were systematically elucidated by the approach of network pharmacology. Methods By using network pharmacology approach, a total of 53 nonredundant bio-active components met the drug screening criteria and 155 putative targets of RKY in treatment of breast cancer were identified. Besides, 381 potential breast cancer-related targets were obtained. Among of the targets, 56 shared targets of RKY and breast cancer were acquired. Results Based on topological network analysis of PPI network of shared targets, 19 hub therapeutic targets of RKY against breast cancer were obtained. GO and KEGG pathway enrichment analysis of core therapeutic targets showed that the core targets remarkably involved in multiple biological functions and KEGG pathways which mainly participated in inflammatory response, cell proliferation, and apoptosis. Conclusions These findings uncover the hub bio-active compounds and underlying mechanisms of RKY against breast cancer and provide crucial information regarding using RKY as a promising candidate for treating breast cancer.

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Kaempferol 7- O - β -D-glucoside isolated from the leaves of Cudrania tricuspidata inhibits LPS-induced expression of pro-inflammatory mediators through inactivation of NF-κB, AP-1, and JAK-STAT in RAW 264.7 macrophages

Cited by 48 publications

References 52 publications

Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

Pterostilbene and 4′-Methoxyresveratrol Inhibited Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages

Network pharmacology reveals hub bio-active ingredients and possible mechanisms of a Chinese herbal prescription Ru-kang-yin against breast cancer

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