Kaempferol as Selective Human MAO-A Inhibitor: Analytical Detection in Calabrian Red Wines, Biological and Molecular Modeling Studies

Abstract

The purpose of this work was to determine the kaempferol content in three red wines of Calabria, a southern Italian region with a great number of certified food products. Considering that wine cultivar, climate, and soil influence the qualitative and quantitative composition in flavonoids of Vitis vinifera L. berries, the three analyzed samples were taken from the 2013 vintage. Moreover, the Gaglioppo samples, with assigned Controlled Origin Denomination (DOC), were also investigated in the production of years 2008, 2010, and 2011. In addition to the analysis of kaempferol, which is present in higher concentration than in other Italian wines, in vitro assays were performed to evaluate, for the first time, the inhibition of the human monoamine oxidases (hMAO-A and hMAO-B). Molecular recognition studies were also carried out to provide insight into the binding mode of kaempferol and selectivity of inhibition of the hMAO-A isoform.

Reveals of New Candidate Active Components in Hemerocallis Radix and Its Anti-Depression Action of Mechanism Based on Network Pharmacology Approach

“…It has been proved to be a potent MAOA and MAOB inhibitor, antioxidant, and presents neuroprotective effect in the mouse model [34,35]. In spite of that, a more recent study showed that kaempferol acts as a selective inhibitor of human MAOA which is consistent with our study [36]. Vanillic acid is a phenolic acid with formula C 8 H 8 O 4 , found in some forms of vanilla and many other plant extracts.…”

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

“…In the nutraceutical field, computational techniques have been successfully used for the prediction of ligand-target binding affinity and to better understand the molecular basis of the biological responses. In silico studies also provide additional insight into the possible mechanism of action and binding mode of active compounds against metabolic key enzymes 82 .…”

Kaempferol Protects Against Cerebral Ischemia Reperfusion Injury Through Intervening Oxidative and Inflammatory Stress Induced Apoptosis

“…Except that, kaempferol was reported can selectively inhibit human monoamine oxidases-A (MAO-A) in brain mitochondrial. The role of MAOs is to catalyze the a-carbon two-electron oxidation of amine substrates in the peripheral tissues and brain (Gidaro et al, 2016). So far, there are still lack of reports about the neuroprotective effect and possible mechanisms of kaempferol on I/R in vivo.…”