Karyometric Changes in Human Muscle with Age

Vassilopoulos, Dimitris; Lumb, Elizabeth; Emery, Alan E. H.

doi:10.1159/000114877

Cited by 22 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The only other study on human myonuclear domain size development that we are aware of reported that the nucleo-cytoplasmic ratio remained constant between 1 and 2.5 years to 12 and 30 years (Vassilopoulos et al, 1977). At first glance this appears at odds with our data, but it should be noted that they used fiber diameter as a measure of fiber size.…”

Section: Discussioncontrasting

confidence: 87%

See 1 more Smart Citation

Steep Increase in Myonuclear Domain Size During Infancy

Delhaas

Meer

Schaart

et al. 2012

The Anatomical Record

View full text Add to dashboard Cite

We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 lm 2 Áper annum (R 2 ¼ 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase.

show abstract

Section: Discussioncontrasting

confidence: 87%

“…In rodents it increases its size 4-fold between 4 days and 3 months after birth (Kelly, 1978;Kawano et al, 2008;White et al, 2010). Though in humans the myonuclear domain has also been found to increase 2.5-fold from toddler to adolescent (Vassilopoulos et al, 1977), a detailed time course of this increase is yet lacking, especially during infancy.…”

mentioning

confidence: 99%

Steep Increase in Myonuclear Domain Size During Infancy

Delhaas

Meer

Schaart

et al. 2012

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Two previous human studies suggested that myonuclei are not lost during normal aging regardless of whether fiber size is altered. Vassilopoulos et al40 obtained muscle biopsies from normal individuals whose ages ranged from 1 to 71 years and reported that changes in muscle fiber size were accompanied by similar changes in nuclear number, which resulted in a constant nucleocytoplasmic ratio in the age range studied. Manta et al22 performed an autopsy study of 15 individuals whose ages ranged from 17 to 82 years and found that the nucleocytoplasmic ratio increased significantly after the age of 60 years, due to a decrease in mean fiber size, whereas the number and the size of myonuclei remained unchanged.…”

Section: Discussionmentioning

confidence: 99%

Satellite cells and myonuclei in young and elderly women and men

Kadi

Charifi²,

Denis³

et al. 2003

Muscle and Nerve

236

188

View full text Add to dashboard Cite

The overall aim of this study was to assess the effects of aging on the satellite cell population. Muscle biopsies were taken from the tibialis anterior muscle of healthy, moderately active young (age range, 20-32 years; n = 31) and elderly (age range, 70-83 years; n = 27) women and men with comparable physical activity pattern. Satellite cells and myonuclei were visualized using a monoclonal antibody against neural cell adhesion molecule and counterstained with Mayer's hematoxylin. An average of 211 (range, 192-241) muscle fibers were examined for each individual. Compared with the young women and men, the elderly subjects had a significantly lower (P < 0.011) number of satellite cells per muscle fiber but a significantly higher (P < 0.004) number of myonuclei per muscle fiber. The number of satellite cells relative to the total number of nuclei [satellite cells/(myonuclei + satellite cells)] was significantly lower in the elderly than in the young women and men. These results imply that a reduction in the satellite cell population occurs as a result of increasing age in healthy men and women.

show abstract

“…A relationship between the number of myonuclei and fibre size has been reported in trained young (Kadi et al, 1999) and old (Manta et al, 1987;Hikida et al, 2000) individuals, but not in untrained elderly subjects (Hikida et al, 1998), reflecting an aging-and ⁄ or atrophy-related deterioration of this relationship (Ohira et al, 1999). In humans, there are a few reports in the literature on the effects of aging on myonuclear density, describing a constant (Vassilopoulos et al, 1977) or decreased (Manta et al, 1987) cytoplasmic density in old age. In addition, an increased myonuclear density has been observed in elderly men and women, but the study did not correct for muscle fibre size (Kadi et al, 2004a,b).…”

Section: Introductionmentioning

confidence: 99%

Effects of aging and gender on the spatial organization of nuclei in single human skeletal muscle cells

et al. 2010

View full text Add to dashboard Cite

SummaryThe skeletal muscle fibre is a syncitium where each myonucleus regulates the gene products in a finite volume of the cytoplasm, i.e., the myonuclear domain (MND). We analysed aging-and gender-related effects on myonuclei organization and the MND size in single muscle fibres from six young (21-31 years) and nine old men (72-96 years), and from six young (24-32 years) and nine old women (65-96 years), using a novel image analysis algorithm applied to confocal images. Muscle fibres were classified according to myosin heavy chain (MyHC) isoform expression. Our image analysis algorithm was effective in determining the spatial organization of myonuclei and the distribution of individual MNDs along the single fibre segments. Significant linear relations were observed between MND size and fibre size, irrespective age, gender and MyHC isoform expression. The spatial organization of individual myonuclei, calculated as the distribution of nearest neighbour distances in 3D, and MND size were affected in old age, but changes were dependent on MyHC isoform expression. In type I muscle fibres, average NN-values were lower and showed an increased variability in old age, reflecting an aggregation of myonuclei in old age. Average MND size did not change in old age, but there was an increased MND size variability. In type IIa fibres, average NN-values and MND sizes were lower in old age, reflecting the smaller size of these muscle fibres in old age. It is suggested that these changes have a significant impact on protein synthesis and degradation during the aging process.

show abstract

Karyometric Changes in Human Muscle with Age

Cited by 22 publications

References 0 publications

Steep Increase in Myonuclear Domain Size During Infancy

Steep Increase in Myonuclear Domain Size During Infancy

Satellite cells and myonuclei in young and elderly women and men

Effects of aging and gender on the spatial organization of nuclei in single human skeletal muscle cells

Contact Info

Product

Resources

About