Karyotype evolution of the clonal rat liver cell line CL 52 during progression in vitro and in vivo

Kerler, R.; Rabes, Hartmut M.

doi:10.1016/0165-4608(95)00215-4

Cited by 4 publications

(4 citation statements)

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“…The marked FR-1 spots are congruent with rat hepatoma-derived AR-like protein (spot 17) and spot 14. As a positive control, GST-P antibody was used.…”

Section: Discussionmentioning

confidence: 92%

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Further Characterization of a Rat Hepatoma‐Derived Aldose‐Reductase‐Like Protein

Zeindl‐Eberhart

Jungblut

Otto

et al. 1997

European Journal of Biochemistry

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A protein detected in N-methyl-N-nitrosourea-initiated rat hepatomas by two-dimensional electrophoresis at 35 kDdpI 7.4 was identified in a previous study by internal amino acid micro sequencing as an aldose-reductase-like protein [Zeindl-Eberhart, E., Jungblut, P. R., Otto, A. & Rabes, H. M. (1994) Identification of tumor-associated protein variants during rat hepatocarcinogenesis, J. Biol. Chem. 269, 14 589-14 5941. Two-dimensional electrophoresis of rat lens proteins revealed a spot at 37 kDa/pI 6.8 that showed a high degree of identity (98.5%) with rat lens aldose reductase after amino acid sequencing and 80% sequence identity to the rat-hepatoma-derived aldose-reductase-like protein. This suggests that hepatomaderived aldose-reductase-like protein and rat lens aldose reductase are related proteins encoded by different genes. A different expression profile of these proteins was found in various rat organs. Rat lens aldose reductase is present, in addition to in lens, in heart, brain, muscle, lung, duodenum, kidney, spleen and bone marrow, while the hepatoma-derived aldose-reductase-like protein is found preferentially in hepatomas and in embryonic liver. Though different in organ expression, an identical response was found for both proteins after stimulation with fibroblast growth factor-1 and after exposure to increased glucose concentrations. Since rat hepatoma-derived aldose-reductase-like protein is expressed in embryonic, but not in adult liver, it is assumed that it is expressed in hepatomas as a functionally active embryonal type of aldose reductase during hepatocarcinogenesis. Immunohistochemistry revealed that the hepatomaderived aldose-reductase-like protein is expressed already in the preneoplastic stage of hepatocarcinogenesis and might potentially serve as a marker enzyme in early hepatic neoplasia.Keywords: two-dimensional electrophoresis ; rat hepatoma; aldose-reductase-like protein.Misprogramming of genetic information in cancer may lead to quantitative andor qualitative protein alterations. Such changes can be studied by high resolution two-dimensional electrophoresis [ 1-31, in combination with ultrasensitive silver staining [4, 51. Two-dimensional electrophoresis has successfully been used to detect tumor associated proteins in chemically induced rat hepatomas [6-121.We detected previously several protein variants in N-methyl-N-nitrosourea-induced rat hepatomas by two-dimensional electrophoresis [ I l l and identified one of these variants (spot 17) by amino acid sequencing as an enzyme apparently related to rat lens aldose reductase (AR) [13]. In a different system, NIH 3T3 mouse fibroblasts, it was shown that fibroblast growth factor-I (FGF-I) induced temporarily an increased level of FGF-1-regulated (FR-1) mRNA [14]. The amino acid sequence deduced from FR-1 mRNA revealed 97% sequence identity [I41 to the rat hepatoma-derived AR-like protein of chemically induced hepatomas [ 131. These findings of putative relations between rat hepatoma-derived AR-like protein, rat lens AR and FR-1 prot...

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“…The marked FR-1 spots are congruent with rat hepatoma-derived AR-like protein (spot 17) and spot 14. As a positive control, GST-P antibody was used.…”

Section: Discussionmentioning

confidence: 92%

“…(b) A congruent part of a two-dimensional electrophoresis gel of rat lens. Rat lens AR (p37) and the position of rat hepatoma-derived AR-like protein (spot 17) are marked by arrows.…”

Section: Methodsmentioning

confidence: 99%

Further Characterization of a Rat Hepatoma‐Derived Aldose‐Reductase‐Like Protein

Zeindl‐Eberhart

Jungblut

Otto

et al. 1997

European Journal of Biochemistry

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“…This diagram allows easy visualization of the ancestral and recent rearrangements, as well as providing an overview of the microevolutionary processes that have occurred in the progression of this tumor cell line. Analyses of karyotype clonal evolution have been performed previously in rats [32], [44], showing its relevance in the investigation of tumor progression. Moreover, the existence of ancestral structural chromosome abnormalities suggests a relevant role for these rearrangements in providing a selective advantage to this tumor cell line.…”

Section: Discussionmentioning

confidence: 99%

Defining the Sister Rat Mammary Tumor Cell Lines HH-16 cl.2/1 and HH-16.cl.4 as an In Vitro Cell Model for Erbb2

2012

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Cancer cell lines have been shown to be reliable tools in genetic studies of breast cancer, and the characterization of these lines indicates that they are good models for studying the biological mechanisms underlying this disease. Here, we describe the molecular cytogenetic/genetic characterization of two sister rat mammary tumor cell lines, HH-16 cl.2/1 and HH-16.cl.4, for the first time. Molecular cytogenetic analysis using rat and mouse chromosome paint probes and BAC/PAC clones allowed the characterization of clonal chromosome rearrangements; moreover, this strategy assisted in revealing detected breakpoint regions and complex chromosome rearrangements. This comprehensive cytogenetic analysis revealed an increase in the number of copies of the Mycn and Erbb2 genes in the investigated cell lines. To analyze its possible correlation with expression changes, relative RNA expression was assessed by real-time reverse transcription quantitative PCR and RNA FISH. Erbb2 was found to be overexpressed in HH-16.cl.4, but not in the sister cell line HH-16 cl.2/1, even though these lines share the same initial genetic environment. Moreover, the relative expression of Erbb2 decreased after global genome demethylation in the HH-16.cl.4 cell line. As these cell lines are commercially available and have been used in previous studies, the present detailed characterization improves their value as an in vitro cell model. We believe that the development of appropriate in vitro cell models for breast cancer is of crucial importance for revealing the genetic and cellular pathways underlying this neoplasy and for employing them as experimental tools to assist in the generation of new biotherapies.

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“…The reason for this discrepancy could be either that we are facing a karyotypic evolution of the cell line since its establishment in 1988, or that Gioanni et al [13] analyzed incomplete metaphases. Many publications have reported on the phenomenon of karyotypic evolution in cell culture [21,22,23,24]. Bahia et al [25] and Watson et al [26], for instance, investigated independently cultured strains of breast cancer cell lines and noticed, besides several common alterations, a significant number of additional and unique aberrations that emerged during in vitro cultivation and genotypic evolution.…”

Section: Resultsmentioning

confidence: 99%

Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC)

Bauer

Hieber

Schaeffner

et al. 2010

Genes

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Cytogenetic analysis of head and neck squamous cell carcinoma (HNSCC) established several biomarkers that have been correlated to clinical parameters during the past years. Adequate cell culture model systems are required for functional studies investigating those potential prognostic markers in HNSCC. We have used a cell line, CAL 33, for the establishment of a cell culture model in order to perform functional analyses of interesting candidate genes and proteins. The cell line was cytogenetically characterized using array CGH, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH). As a starting point for the investigation of genetic markers predicting radiosensitivity in tumor cells, irradiation experiments were carried out and radiation responses of CAL 33 have been determined. Radiosensitivity of CAL 33 cells was intermediate when compared to published data on tumor cell lines.

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Karyotype evolution of the clonal rat liver cell line CL 52 during progression in vitro and in vivo

Cited by 4 publications

References 32 publications

Further Characterization of a Rat Hepatoma‐Derived Aldose‐Reductase‐Like Protein

Further Characterization of a Rat Hepatoma‐Derived Aldose‐Reductase‐Like Protein

Defining the Sister Rat Mammary Tumor Cell Lines HH-16 cl.2/1 and HH-16.cl.4 as an In Vitro Cell Model for Erbb2

Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC)

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