KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)

Radanović, Aleksandra; Sprycha, Yves; Jocković, Milan; Sundt, Monja; Miladinović, Dragana; Jansen, Constantin; Horn, Renate

doi:10.3390/genes13030465

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…state-of-the-art and high-throughput technologies for markerassisted selection, which are widely used in plant breeding (Broccanello et al, 2018;Radanovićet al, 2022). KASP technology is known to provide a lower cost per data point, however it provides a less efficient separation of genotypic clusters and requires higher amount of DNA template (Ayalew et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Detection and distribution of two dominant alleles associated with the sweet kernel phenotype in almond cultivated germplasm

et al. 2023

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Almond [Prunus dulcis Miller (D. A. Webb), syn. Prunus amygdalus L.)] is the major tree nut crop worldwide in terms of production and cultivated area. Almond domestication was enabled by the selection of individuals bearing sweet kernels, which do not accumulate high levels of the toxic cyanogenic glucoside amygdalin. Previously, we showed that the Sweet kernel (Sk) gene, controlling the kernel taste in almond, encodes a basic helix loop helix (bHLH) transcription factor regulating the amygdalin biosynthetic pathway. In addition, we characterized a dominant allele of this gene, further referred to as Sk-1, which originates from a C1036→T missense mutation and confers the sweet kernel phenotype. Here we provide evidence indicating that the allele further referred to as Sk-2, originally detected in the cultivar “Atocha” and arising from a T989→G missense mutation, is also dominantly inherited and confers the sweet kernel phenotype in almond cultivated germplasm. The use of single nucleotide polymorphism (SNP) data from genotyping by sequencing (GBS) for population structure and hierarchical clustering analyses indicated that Sk-2 occurs in a group of related genotypes, including the widespread cultivar “Texas”, descending from the same ancestral population. KASP and dual label functional markers were developed for the accurate and high-throughput selection of the Sk-1 and Sk-2 alleles, and the genotyping of a panel of 134 almond cultivars. Overall, our results provide further insights on the understanding of the almond cultivation history. In addition, molecular marker assays and genotypic data presented in this study are expected to be of major interest for the conduction of almond breeding programs, which often need to select sweet kernel individuals in segregant populations.

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Section: Discussionmentioning

confidence: 99%

Detection and distribution of two dominant alleles associated with the sweet kernel phenotype in almond cultivated germplasm

et al. 2023

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“…In sunflower, four KASP markers were detected by Radanović et al . (2022), having a role in male fertility and will be useful for MAS breeding.…”

Section: Potential Approaches To Improve Ht Stress Tolerance In Plantsmentioning

confidence: 99%

High‐temperature stress in crops: male sterility, yield loss and potential remedy approaches

Khan

Ling

et al. 2022

Plant Biotechnology Journal

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Summary Global food security is one of the utmost essential challenges in the 21st century in providing enough food for the growing population while coping with the already stressed environment. High temperature (HT) is one of the main factors affecting plant growth, development and reproduction and causes male sterility in plants. In male reproductive tissues, metabolic changes induced by HT involve carbohydrates, lipids, hormones, epigenetics and reactive oxygen species, leading to male sterility and ultimately reducing yield. Understanding the mechanism and genes involved in these pathways during the HT stress response will provide a new path to improve crops by using molecular breeding and biotechnological approaches. Moreover, this review provides insight into male sterility and integrates this with suggested strategies to enhance crop tolerance under HT stress conditions at the reproductive stage.

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Kompetitive Allele-Specific PCR (KASP): An Efficient High-Throughput Genotyping Platform and Its Applications in Crop Variety Development

Rahman

Hasan

Rahman

2023

Molecular Marker Techniques

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KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)

Cited by 6 publications

References 33 publications

Detection and distribution of two dominant alleles associated with the sweet kernel phenotype in almond cultivated germplasm

Detection and distribution of two dominant alleles associated with the sweet kernel phenotype in almond cultivated germplasm

High‐temperature stress in crops: male sterility, yield loss and potential remedy approaches

Kompetitive Allele-Specific PCR (KASP): An Efficient High-Throughput Genotyping Platform and Its Applications in Crop Variety Development

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