Keratin pattern in hyperkeratotic and ulcerated gastric pars oesophagea in pigs

Roels, Stefan; Ducatelle, Richard; Broekaert, Daniël

doi:10.1016/s0034-5288(97)90140-5

Cited by 14 publications

(7 citation statements)

References 21 publications

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“…KRT5-like protein is 70% similar to KRT5 and their functions may be similar. KRT5 expression is specific to basal and epibasal cells of the stratified squamous epithelium and can be observed in ulceration at the nonglandular part of porcine stomach (pars oesophagea) [42].…”

Section: Discussionmentioning

confidence: 99%

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry

Tesena

Yingchutrakul

Roytrakul

et al. 2019

Equine Veterinary Journal

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Summary Background Equine squamous gastric disease (ESGD) is a very common disorder but an accurate and practical screening technique for detecting ESGD is currently lacking. Objectives To identify serum protein markers to detect ESGD using electrophoresis and mass spectrometry. Study design Proteomic analysis and bioinformatics. Methods ESGD was diagnosed using gastroscopy in 30 horses. Gastric ulceration was categorised into three groups: normal, mild/moderate and severe ESGD. Pooled sera from each group were compared using 1D electrophoresis and mass spectrometry. The candidate proteins for ESGD markers were selected based on their specifically high expression in nonglandular stomach, and their association with gastric ulceration using public gene and protein databases. Results The prevalence of ESGD in this study was 43% (with mild/moderate ESGD at 33% and severe ESGD at 10%). The proteomic study revealed that the identified serum protein markers for normal equine stomach were B4GALNT2 and XDH. The marker for mild/moderate EGSD was KRT10, while the marker for severe ESGD was KLK13. Furthermore, markers for both ulcer types were SLC4A7, PPARG, FCGBP, PKP1, ASPRV1 and KRT5‐like proteins. Main limitations The functions of the identified proteins are not well characterised in horse. Proteomics is a tool for screening protein markers, but confirmation of putative protein markers with specific antibodies is required. Conclusions In total, 10 serum proteins found in this study may be used as putative markers for ESGD. However, confirmation of candidate proteins with specific antibodies in a larger study cohort is necessary before it can be used in the veterinary clinic or on horse farms. The Summary is available in Portuguese – see Supporting Information

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Section: Discussionmentioning

confidence: 99%

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry

Tesena

Yingchutrakul

Roytrakul

et al. 2019

Equine Veterinary Journal

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“…KRT1 is associated with chronic disease such as inflammatory bowel disease in humans [ 7 ]. KRT6A expression is important and high during wound healing in the stratified squamous epithelium [ 6 , 40 ] and associated with mucosal change, ulceration, and hyperkeratosis in the oesophagus and pars oesophagea of pigs [ 13 , 25 ], where it has a similar structure to the non-glandular part of the equine stomach [ 2 , 8 ].…”

Section: Discussionmentioning

confidence: 99%

Optimisation of a serum albumin removal protocol for use in a proteomic study to identify the protein biomarkers for silent gastric ulceration in horses

Poltep

Tesena

Yingchutrakul

et al. 2018

JES

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Silent gastric ulceration occurs without evidence of clinical signs and is common in horses. There is currently no a simple and effective method to diagnose this disease. Proteomics can be used to identify serum biomarkers, but the most abundant serum protein, albumin, could conceal candidate biomarkers. Therefore, it is recommended to remove albumin before a proteomic study; however, there is no specific albumin depletion kit or standard protocol available for horse samples. The objectives of this study were to optimise a protocol to remove equine serum albumin and to use albumin-depleted serum to identify the protein biomarkers for silent gastric ulceration. Gastroscopy was used to identify gastric ulceration, and serum was obtained from horses with either a healthy gastric mucosa or gastric ulceration. Serum albumin was removed using the trichloroacetic acid (TCA) protein precipitation method, and this protocol was optimised by varying the concentration of TCA, type of organic solvents, ratio of serum to protein precipitation solution, and incubation times. Electrophoresis and image analysis were used to compare the amounts of albumin, immunoglobulins G (IgG), and protein degradation before and after TCA precipitation. The best protocol was chosen to remove albumin for a proteomic study (electrophoresis and mass spectrometry). The results revealed that protocol 2 (ratio of serum to solution 1:5, 10% TCA in acetone, and 90 min incubation) was the most efficient protocol to remove albumin (98%) and IgG heavy (80%) and light (98%) chains without degrading other proteins. After electrophoresis and mass spectrometry analysis, KRT1, KRT6A and KRT18 were identified as potential markers for silent gastric ulceration.

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“…Hyperkeratosis is known to be a pre-ulcerous lesion that occurs because of abnormalities of keratinisation caused by conditions disturbing the intricate control over the gastric epithelium (O’Sullivan et al . 1996 ; Roels & Ducatelle 1997 ). Gross erosion mostly affected the cardia and the fundus, whilst lacerations and scars were more frequently observed in the fundus.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, distribution and pattern of gastric lesions in slaughtered pigs in south-western Nigeria

Omotosho

Emikpe

Lasisi

et al. 2016

Onderstepoort J Vet Res

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Gastric lesions, especially ulceration, cause significant economic losses in the swine industry worldwide. The study was designed to assess its prevalence, distribution and pattern in pigs in south-western Nigeria. Slaughter house surveys were conducted on three government-established abattoirs in Lagos, Ogun and Oyo states. Stomachs from 480 pigs were assessed for gross lesions, which were graded using a modification of a standard technique. Tissues from different regions of the stomach were routinely stained to assess histopathologic changes. Data were presented as frequency counts and analysed using analysis of variance and chi-square technique. Significance was determined at p ≤ 0.05. Gastric lesions were encountered across the four regions of the stomach with a point prevalence of 57.29%. The prevalence of lesions in the non-glandular region was 32.9%, with severe hyperkeratosis (13.13%) being most frequently observed (p < 0.05). Erosions were significantly higher in the cardia (8.54%) (p < 0.05), followed by fundus (8.33%). Gastric ulcers were significantly higher in the fundus (19.58%) (p < 0.05). Scars of healed ulcers and lacerations were also observed in the fundus (5.42%) (p < 0.05). The gastric lesion distribution across the four regions of the stomach and the occurrence of ulceration in the fundus showed an unusual pattern, which is rarely reported in other parts of the world. The reason for these findings in pigs in Nigeria is not fully understood; therefore, further studies are required to identify and manage these factors for increased productivity, improved animal welfare and enhanced food security.

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Keratin pattern in hyperkeratotic and ulcerated gastric pars oesophagea in pigs

Cited by 14 publications

References 21 publications

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry

Optimisation of a serum albumin removal protocol for use in a proteomic study to identify the protein biomarkers for silent gastric ulceration in horses

Prevalence, distribution and pattern of gastric lesions in slaughtered pigs in south-western Nigeria

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