Keratocyte density and size in conscious humans by digital image analysis of confocal images

Abstract

Purpose Confocal microscopy can give images of high magnification and resolution in undisturbed living tissue. It provides new information about the cellular structure of the cornea. Our aim was to measure the density, size and distribution of keratocytes.Methods Healthy cornea in four subjects was examined using tandem scanning confocal microscopy. Methods for digital analysis of images were developed.Results Keratocyte density in confocal cross sections was greatest immediately under

Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy

“…[58][59][60][61][62] Keratoctye density, in addition, has been reported to decrease with increasing age, 60,62,64 however, other authors 57,63 have reported consistency in the corneal keratocyte populations throughout life. In this study, reduction in keratocyte density with age was found to be significant in the posterior stroma only, with a strong trend within the anterior stroma.…”

Corneal Cell Density Measurement in vivo by Scanning Slit Confocal Microscopy: Method and Validation

“…The methods applied range from manual techniques based on cell marking or cell border tracing [8,13,14] to fully automated cell identification and counting [15][16][17]. The acceptance of CoCM as a method for cell size and density measurements implies validation of the results against equivalent results obtained by histological techniques and knowledge about the reliability and repeatability of the confocal measurements.…”

In Vivo Confocal Microscopic Evaluation of Cells and Nerve Fibers of the Healthy Human Cornea

“…19,20 Bu çalışmada önceki çalışmaların sonuçlarına benzer şekilde, keratosit dansitelerinde kadın ve erkek cinsiyet arasında anlamlı farklılık görülmemiştir. 10,11,17 Yaş ile birlikte keratosit dansitesinde azalma olduğu ise birçok çalışmada bildirilmiştir.…”