Kerong fish (<i>Terapon jarbua</i>) peptone production using papain enzyme as nitrogen source in bacterial media

Srikandace, Yoice; Priatni, Sri; Pudjiraharti, Sri; Kosasih, Wawan; Indrarti, Lucia

doi:10.1088/1755-1315/60/1/012005

Cited by 5 publications

(6 citation statements)

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“…Introduction N umerous researchwork has been performed to estimate the proximate composition of fish (Kumaran et al, 2012;Hernandez et al, 2018;Khalid and Naeem, 2018;Iqbal et al, 2020), and outcomes from some of these have been utilized to create the nutritional needs in fishes (Okumuş and Mazlum, 2002;Tidwell et al, 2007). Terapon jarbua fish is a widely distributed species, dispersed mostly in the region of Indo-West Pacific and exist in low coastal waters areas, freshwater and mangroves (Srikandace et al, 2017).…”

Section: Research Articlementioning

confidence: 99%

Proximate Composition Analysis of Marine Fish, Terapon jarbua, from Pakistan

Lal¹,

Naeem²

2021

SJA

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| Extensive research work has been done on proximate analysis of the fish around the world but very little analysis on marine fish Terapon jarbua have been performed. Therefore, the current analysis was carried out to analyze the proximate composition of T. jarbua. 70 samples of T. jarbua ranging from 18.00-29.90 cm in length and 119.60-429.50 g in body weight were collected from Fisheries Harbour, Karachi Pakistan. The mean percentage of constituents were found to be for water 73.22%, ash 6.72%, fat 3.45% and protein 16.62% in the whole wet weight of body of T. jarbua. % water represented correlation with highly significant value (P<0.001) with protein, ash, fat and organic constituents in wet weight. Fish body weight represented highly significant (P<0.001) positive relation to all the studied body constituents in log transformed data. Total length also represented highly-significant positive relation to all the studied body constituents except for fat which showed nonsignificant correlation (P>0.05). Fulton's condition factor showed nonsignificant relations to whole percent (%) body constituents except percent (%) ash, which showed least significant relation (P<0.05) for T. jarbua.

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Section: Research Articlementioning

confidence: 99%

Proximate Composition Analysis of Marine Fish, Terapon jarbua, from Pakistan

Lal¹,

Naeem²

2021

SJA

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“…Both bacteria did not grow well in the resultant peptone media, as shown by total colony of each bacteria of 26x10 -5 and 14x10 -5 , respectively. Previous study showed that the growth of S. aureus using fish peptone from T. jarbua was lower than that of commercial peptone [5]. It also indicated that fish peptone of T. jarbua was not suitable for the growth media of E. cloacae and S. aureus.…”

Section: Microbiology Analysismentioning

confidence: 98%

“…This type of fish is usually sold as low-valued products even though they contain proteins and essential amino acids [3,4]. The T. jarbua fish contains water soluble protein hydrolysate in mixture of polypeptides and amino acids such as peptone [5]. Peptone is a cheap material for microbial growth medium and eco-friendly.…”

Section: Introductionmentioning

confidence: 99%

“…Preliminary study [5] showed that the highest yield of peptone from T. jarbua was achieved by 0.2% w/w of non-commercial papain enzyme hydrolysis at pH 7. Therefore, this present study aims to optimize the production of T. jarbua fish peptone using commercial alcalase and papain enzyme at varied temperature of 50-65°C, hydrolysis time (4-7 h), as well as to characterize the fish peptone product.…”

Section: Introductionmentioning

confidence: 99%

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The production of Kerong fish (Terapon jarbua) peptone using enzymatic hydrolysis

Srikandace

Priatni

Pudjiraharti

et al. 2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. The Kerong fish (Terapon jarbua) peptone was produced by using alcalase and papain enzymes. The parameters of fish peptone development were temperature (50-65°C), pH 7, and hydrolysis time (4-7 h). Papain and alcalase were used to digest the substrate with concentrations of 0.2% (w/w). The results showed that the highest yield of peptone was resulted by using alcalase and papain enzymes at 65°C for 6 h and 5 h, respectively. The maximum harvest of peptone using alcalase enzyme was shown by soluble protein content of 6.382 mg/ml with N total of 0.535. The highest peptone production using papain enzyme revealed soluble protein content of 5.781 mg/ml with N total of 0.252. It indicated that alcalase enzyme produced more fish peptone T. jarbua than papain enzyme. The resultant fish peptone supported the growth of Pseudomonas aeruginosa, Salmonella thypii, Bacillus subtilis and Clostridium sporogenes.

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“…Deraz et al (2011) used tilapia viscera for peptone production and showed a high biomass production. Srikandace et al (2017) reported that peptone extracted from kerong fish (Terapon jarbua) showed a higher growth rate in E. coli compared to commercial peptone. These findings are also in line with the peptone isolated from Atlantic cod (Gadus morhua) stomach with formic and phosphoric acid reported by Gildberg et al (2010).…”

Section: Introductionmentioning

confidence: 99%

Characteristics and Use of Peptones from Catfish (Clarias gariepinus) and Pangas Catfish (Pangasius pangasius) Heads as Bacterial Growth Media

Setijawati¹,

Jaziri²,

Yufidasari³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Peptone is a hydrolysate product rich in amino acids, and it is uncoagulated at high temperature. Commercial peptone produced from land animals cannot be declared as acceptable in terms of lawfulness due to religious concerns. Catfish (Clarias gariepinus) and pangas catfish (Pangasius pangasius) are important species for the fish processing industry in Indonesia. The filleting process resulted in value by-products. The fish head as the by-products can be utilized as a main raw material for higher economic value products, such as peptone. The aim of this study was to characterize peptones extracted from the heads of catfish and pangas catfish with different acid conditions. The characteristics of chemical composition, yield, color parameter, solubility, amino acid content, bacterial growth rate and biomass production were observed. The catfish peptone (CFP) and pangas catfish peptone (PCP) obtained with different acid conditions showed high protein content in the range of 84.35% to 90.80% (P<0.05). The yields of CFP and PCP were significantly different (P<0.05) and varied between 4.75% and 5.66%. The solubility of treated peptones varied between 98.03% and 99.52%, and the peptones were rich in glycine, glutamic acid, proline and leucine. Bacterial growth test showed that both CFP and PCP had better growth rates compared to the commercial peptone tested in this study. In addition, the biomass production with peptone from catfish and pangas catfish was higher than that with the commercial product (P<0.05). This research proposed that catfish and pangas catfish heads could be developed as an alternative source for peptone production.

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Kerong fish (Terapon jarbua) peptone production using papain enzyme as nitrogen source in bacterial media

Cited by 5 publications

References 5 publications

Proximate Composition Analysis of Marine Fish, Terapon jarbua, from Pakistan

Proximate Composition Analysis of Marine Fish, Terapon jarbua, from Pakistan

The production of Kerong fish (Terapon jarbua) peptone using enzymatic hydrolysis

Characteristics and Use of Peptones from Catfish (Clarias gariepinus) and Pangas Catfish (Pangasius pangasius) Heads as Bacterial Growth Media

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