Ketamine inhibits nitric oxide synthase in lipopolysaccharide-treated rat alveolar macrophages

Li, Chi Yuan; Chou, Teh‐Chang; Wong, Chih Shung; Ho, Shung Tai; Wu, Chang‐Chieh; Yen, Mao‐Hsiung; Ding, Yu An

doi:10.1007/bf03011971

Cited by 52 publications

(29 citation statements)

References 27 publications

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“…Our findings that ketamine does not reduce cytokine-mediated upregulation of iNOS expression after PHT is not in accordance with studies reporting the inhibitory effect of ketamine on iNOS-mediated NO release from alveolar macrophages in response to lipopolysaccharide in vitro 42,43 . The effect of ketamine on iNOS expression in vivo may, therefore, be a result of additional mechanisms than those suggested by in vitro experiments, and further study in this area is merited.…”

Section: ■ Discussioncontrasting

confidence: 90%

Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

et al. 2017

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Purpose: To determine the effects of propofol and ketamine anesthesia on liver regeneration in rats after partial hepatectomy (PHT). Methods: Male Wistar albino rats were assigned randomly to four groups of 10. Anesthesia was induced and maintained with propofol in groups 1 and 2, and with ketamine in groups 3 and 4. PHT was undertaken in groups 1 and 3. Rats in groups 2 and 4 (control groups) underwent an identical surgical procedure, but without PHT. At postoperative day-5, rats were killed. Regenerated liver was removed, weighed, and evaluated (by immunohistochemical means) for expression of inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), apoptosis protease-activating factor (APAF)-1, and proliferating cell nuclear antigen (PCNA). Also, blood samples were collected for measurement of levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Results: Between groups 2 and 4, there were no differences in tissue levels of iNOS, eNOS, and APAF-1 or plasma levels of TNF-α and IL-6. eNOS expression was similar in group 1 and group 3. Expression of iNOS and APAF-1 was mild-to-moderate in group 1, but significantly higher in group 3. Groups 1 and 3 showed an increase in PCNA expression, but expression in both groups was comparable. Plasma levels of TNF-α and IL-6 increased to a lesser degree in group 1 than in group 3. Conclusion: Propofol, as an anesthetic agent, may attenuate cytokine-mediated upregulation of iNOS expression and apoptosis in an animal model of liver regeneration after partial hepatectomy.

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Section: ■ Discussioncontrasting

confidence: 90%

Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

et al. 2017

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“…Thus, we selected ketamine to compare the effect of propofol on oxidative stress. Our finding indicating that ketamine was not reduced iNOS-mediated NO production after complete UO is inconsistent with earlier studies reporting inhibitory effects of ketamine on the iNOS-mediated NO release from alveolar macrophages in response to lipopolysaccharide in vitro [39,40]. The effect of ketamine on NO production from iNOS in vivo may thus be a result of additional mechanisms than those suggested by in vitro experiments, and further study in this area is merited.…”

Section: Discussioncontrasting

confidence: 74%

Preventive effects of propofol and ketamine on renal injury in unilateral ureteral obstruction

et al. 2010

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“…Isoflurane pretreatment has also been shown to prevent endothelial and vascular smooth muscle cell injury in vitro (15) and in vivo (36). Ketamine-xylazine has been shown to attenuate gastrointestinal inducible nitric oxide synthase (iNOS) expression (28) and activity in activated alveolar macrophages exposed to LPS (32). Furthermore, volatile anesthetics have been implicated in the reduction of mRNA and protein levels of iNOS and NOS activity after LPS or gamma interferon stimulation (47).…”

Section: Discussionmentioning

confidence: 99%

General Anesthesia Delays the Inflammatory Response and Increases Survival for Mice with Endotoxic Shock

Fuentes

Talamini

Fulton

et al. 2006

Clin Vaccine Immunol

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Anesthesia is an indispensable component of any operative procedure. In this study, we demonstrate that continuous isoflurane anesthesia for 1 h after a lethal dose (20 mg/kg of body weight) of Escherichia coli lipopolysaccharide (LPS) results in a significant increase in survival of C57BL/6J (B6) mice in comparison with survival of nonanesthetized mice. Protection by anesthesia correlates with a delay in plasma LPS circulation, resulting in a delayed inflammatory response, particularly DNA binding activity of NF-B and serum levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-10. Disparate classes of anesthetic agents produce the same effects on the inflammatory response, which is also independent of the inbred mouse strain used. These results suggest that anesthesia has an important impact on the outcome from endotoxemia. Moreover, the immunomodulatory effects of anesthetics should be considered when interpreting data from experimental animal models.

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Ketamine inhibits nitric oxide synthase in lipopolysaccharide-treated rat alveolar macrophages

Cited by 52 publications

References 27 publications

Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

Preventive effects of propofol and ketamine on renal injury in unilateral ureteral obstruction

General Anesthesia Delays the Inflammatory Response and Increases Survival for Mice with Endotoxic Shock

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