Key indicators for renewal and reconstruction of perennial trees soil: Microorganisms and phloridzin

Li, Xiang; Wang, Mei; Jiang, Weitao; Wang, Yanfang; Chen, Xuesen; Yin, Chengmiao

doi:10.1016/j.ecoenv.2021.112723

Cited by 31 publications

(38 citation statements)

References 58 publications

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“…Mortierella , due to its ability to degrade organic pollutants, can be used for soil remediation. Mortierella has also been detected in the rhizosphere and bulk soils of many plants ( Liu et al, 2021 ; Tong et al, 2021 ; Xiang et al, 2021 ; Zhou et al, 2021 ). Some studies have indicated that Mortierella is related to soil disease inhibition, and may inhibit the diseases caused by Fusarium and participate in the transformation of phosphorus in soil.…”

Section: Introductionmentioning

confidence: 99%

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Wang

Suo

et al. 2022

Front. Microbiol.

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Plant-associated microbes play important roles in plant health and disease. Mortierella is often found in the plant rhizosphere, and its possible functions are not well known, especially in medical plants. Mortierella alpina isolated from ginseng soil was used to investigate its effects on plant disease. The promoting properties and interactions with rhizospheric microorganisms were investigated in a medium. Further, a pot experiment was conducted to explore its effects on ginseng root rot disease. Physicochemical properties, high-throughput sequencing, network co-occurrence, distance-based redundancy analysis (db-RDA), and correlation analysis were used to evaluate their effects on the root rot pathogen. The results showed that Mortierella alpina YW25 had a high indoleacetic acid production capacity, and the maximum yield was 141.37 mg/L at 4 days. The growth of M. alpina YW25 was inhibited by some probiotics (Bacillus, Streptomyces, Brevibacterium, Trichoderma, etc.) and potential pathogens (Cladosporium, Aspergillus, etc.), but it did not show sensitivity to the soil-borne pathogen Fusarium oxysporum. Pot experiments showed that M. alpina could significantly alleviate the diseases caused by F. oxysporum, and increased the available nitrogen and phosphorus content in rhizosphere soil. In addition, it enhanced the activities of soil sucrase and acid phosphatase. High-throughput results showed that the inoculation of M. alpina with F. oxysporum changed the microbial community structure of ginseng, stimulated the plant to recruit more plant growth-promoting bacteria, and constructed a more stable microbial network of ginseng root. In this study, we found and proved the potential of M. alpina as a biocontrol agent against F. oxysporum, providing a new idea for controlling soil-borne diseases of ginseng by regulating rhizosphere microorganisms.

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Section: Introductionmentioning

confidence: 99%

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Wang

Suo

et al. 2022

Front. Microbiol.

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“…OTU closely related to fungi of the genera Mortierella, Cercophora, Pseudogymnaoascus and unidentified Helotiales were associated with nematodes in ARD soils and correlated with reduced root weight. Notably, the genus Mortierella has been repeatedly linked with ARD soils from different orchards [79,80]. Species of Mortierella were reported to colonize apple roots, leading to the conclusion that they may be associated with the onset of ARD [55,81].…”

Section: Discussionmentioning

confidence: 99%

Nematode–Microbe Complexes in Soils Replanted with Apple

et al. 2022

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Apple replant disease is a severe problem in orchards and tree nurseries. Evidence for the involvement of a nematode–microbe disease complex was reported. To search for this complex, plots with a history of apple replanting, and control plots cultivated for the first time with apple were sampled in two fields in two years. Shoot weight drastically decreased with each replanting. Amplicon sequencing of the nematode community and co-extracted fungal and bacterial communities revealed significant differences between replanted and control plots. Free-living nematodes of the genera Aphelenchus and Cephalenchus and an unidentified Dorylaimida were associated with replanted plots, as indicated by linear discriminant analysis effect size. Among the co-extracted fungi and bacteria, Mortierella and Methylotenera were most indicative of replanting. Some genera, mostly Rhabditis, Streptomyces and a fungus belonging to the Chaetomiaceae indicated healthy control plots. Isolating and investigating the putative disease complexes will help to understand and alleviate stress-induced root damage of apple in replanted soil.

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“…The physical and chemical properties and nutritional characteristics of the soil were measured as described by Xiang et al (2021) and Zhang et al (2021) . The nitrogen in the soil was determined by the Kjeldahl method ( Bremner, 1960 ), and the pH and electrolytic conductivity were measured using a sample to water ratio of 1:2.5 (w/v) with a PHS-3E digital pH meter (LEICI, Shanghai, China) and DDSJ-318 conductivity meter (Lei Magnetic, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…Extraction of phenolic acids from soil using the method of Yin et al (2013). The HPLC analysis procedure followed that described by Xiang et al (2021). An UltiMate 3000 HPLC system (Dionex, United States) was used for quantification with a Symmetry R C18 column (4.6 mm × 250 mm, 5.0 µm; Waters, Milford, MA, United States), and a column temperature of 30 • C. Mobile phase A was acetonitrile, and mobile phase B was water (adjusted to pH 2.8 with acetic acid).…”

Section: Soil Treatment With Microbes and Analysis Of Phenolic Acid D...mentioning

confidence: 99%

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

et al. 2022

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In this study, an endophytic phlorizin-degrading Bacillus licheniformis XNRB-3 was isolated from the root tissue of healthy apple trees, and its control effect on apple replant disease (ARD) and how it alleviates the pathogen pressure via changes in soil microbiomes were studied. The addition of strain XNRB-3 in Fusarium infested soils significantly reduced the number of pathogens in the soil, thus resulting in a lower disease incidence, and the relative control effect on Fusarium oxysporum reached the highest of 66.11%. The fermentation broth can also protect the roots of the plants from Fusarium oxysporum, Fusarium moniliforme, Fusarium proliferatum, and Fusarium solani infection. These antagonistic effects were further validated using an in vitro assay in which the pathogen control was related to growth and spore germination inhibition via directly secreted antimicrobial substances and indirectly affecting the growth of pathogens. The secreted antimicrobial substances were identified using gas chromatography-mass spectrometry (GC-MS) technology. Among them, alpha-bisabolol and 2,4-di-tert-butylphenol had significant inhibitory effects on many planted pathogenic fungi. Butanedioic acid, monomethyl ester, and dibutyl phthalate promoted root development of Arabidopsis plants. Strain XNRB-3 has multifarious plant growth promoting traits and antagonistic potential. In pot and field experiments, the addition of strain XNRB-3 significantly promoted the growth of plants, and the activity of enzymes related to disease resistance [superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)] was also significantly enhanced. It also reduced the abundance of four species of Fusarium and the content of phenolic acids in the rhizosphere soil, improved soil microbial community structure and nutritional conditions, and increased soil microbial diversity and activity, as well as the soil enzyme activity. The above results indicated that B. licheniformis XNRB-3 could be developed into a promising biocontrol and plant-growth-promoting agent.

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Key indicators for renewal and reconstruction of perennial trees soil: Microorganisms and phloridzin

Cited by 31 publications

References 58 publications

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng

Nematode–Microbe Complexes in Soils Replanted with Apple

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

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