Key microRNAs and target genes involved in regulating maturation in &lt;i&gt;Lilium&lt;/i&gt;

Chen, Yao; Zhao, Mengna; Wang, Xiaoshan; Cui, Jinteng; Ge, Wei; Zhang, Kezhong

doi:10.48130/opr-2022-0009

Cited by 4 publications

(1 citation statement)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Lilium × formolongi , 5S RNA and EF were selected as reference genes 19 . In the OT (Oriental × Trumpet) hybrid ‘Robina’, U4 was reported as the reference gene 20 . Among the bicolor cultivars of Asiatic hybrid lily, namely, Lollypop, Cancún, Vermeer, Sugar Love, and Enjoys, as well asnthree full-color cultivars of the species, namely, Côte d’Azur, Vivaldi, and Montreux, U6 was identified as the reference gene 21 .…”

Section: Introductionmentioning

confidence: 99%

Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker

Jin,

Zhang,

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Dynamic miRNA detection using the qRT-PCR technique requires appropriate reference genes to ensure data reliability. Previous studies have screened internal reference genes in plants during embryonic development and various stress treatment, involving relatively few tissues and organs. There is no relevant miRNA study in Lilium henryi Baker and limited research on the optimal miRNA reference genes in lilies, such as 5S, 18S, U6 and Actin. Twelve genes were selected as candidate reference genes whose expression stability was analyzed in petals at different developmental stages and other tissues using various algorithms, such as geNorm, NormFinder, BestKeeper, and Delta CT. The results revealed that the optimal combination of reference genes for Lilium henryi Baker petals at different developmental stages was osa-miR166m and osa-miR166a-3p, while that for different tissues of Lilium henryi Baker was osa-miR166g-3p and osa-miR166a-3p.Four important genes related to growth and development regulation, namely, osa-miR156a, osa-miR395b, osa-miR396a-3p, and osa-miR396a-5p, were selected for validation. The findings of the present study could contribute to future investigations onmiRNA expression and the related functions in Lilium henryi Baker while providing important references for the normalization of the miRNA expression in other varieties of lily.

show abstract

Section: Introductionmentioning

confidence: 99%