Ki-67/MKI67 as a Predictive Biomarker for Clinical Outcome in Gastric Cancer Patients: an Updated Meta-analysis and Systematic Review involving 53 Studies and 7078 Patients

Xiong, Dandan; Zeng, Chu‐Mei; Jiang, Ling; Luo, Dan; Chen, Gang

doi:10.7150/jca.30074

Cited by 38 publications

(36 citation statements)

References 63 publications

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“…Marker of proliferation Ki-67 (MKI67) is a proliferation-related nucleus protein gene that is widely expressed in proliferating cells [52]. Previous studies have reported the abnormally high expression of MKI67 in multiple types of cancer, including CRC, and found that MKI67 could be an independent prognostic biomarker in these types of cancer [53]. However, these genes above in our study were not significantly associated with the prognosis in CRC patients.…”

Section: Mki67contrasting

confidence: 77%

CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis

Wang

et al. 2020

World J Surg Onc

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Background: Colorectal cancer (CRC) is one of the most common malignancies of the digestive system, which causes severe financial burden worldwide. However, the specific mechanisms involved in CRC are still unclear. Methods: To identify the significant genes and pathways involved in the initiation and progression of CRC, the microarray dataset GSE126092 was downloaded from Gene Expression Omnibus (GEO) database, and then, the data was analyzed to identify differentially expressed genes (DEGs). Subsequently, the Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on these DEGs using the DAVID database, and the protein-protein interaction (PPI) network was constructed using the STRING database and analyzed using the Cytoscape software. Finally, hub genes were screened, and the survival analysis was performed on these hub genes using the Kaplan-Meier curves in the cBioPortal database. Results: In total, 937 DEGs were obtained, including 316 upregulated genes and 621 downregulated genes. GO analysis revealed that the DEGs were mostly enriched in terms of nuclear division, organelle fission, cell division, and cell cycle process. KEGG pathway analysis showed that the DEGs were mostly enriched in cell cycle, oocyte meiosis, cytokine-cytokine receptor interaction, and cGMP-PKG signaling pathway. The PPI network comprised 608 nodes and 3100 edges, and 4 significant modules and 10 hub genes with the highest degree were identified using the Cytoscape software. Finally, survival analysis showed that overexpression of CDK1 and CDC20 in patients with CRC were statistically associated with worse overall survival. Conclusions: This bioinformatics analysis revealed that CDK1 and CDC20 might be candidate targets for diagnosis and treatment of CRC, which provided valuable clues for CRC.

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Section: Mki67contrasting

confidence: 77%

CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis

Wang

et al. 2020

World J Surg Onc

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“…Xiong retrospectively analyzed multiple studies of 7,078 patients and found that high expression of MKI67 reduced the overall survival rate and tumor‐free survival rate. Further functional analysis revealed that MKI67 might take part in the occurrence of apoptosis by regulating the p53 signaling pathway (Xiong, Zeng, Jiang, Luo, & Chen, 2019). Furthermore, Zeng demonstrated that SNRPA1 (OMIM: 603521) can regulate PIK3R1 (OMIM:171833) , VEGFC (OMIM:601528) , and MKI67 molecules in colorectal cancer through gene overexpression and gene knockout experiments, and then be involved in tumorigenesis and development, suggesting that MKI67 may serve as a molecular target for cell cycle and apoptosis (Zeng et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis and verification of gene targets for benign tracheal stenosis

Wang

Qiu

et al. 2020

Molec Gen & Gen Med

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Background Tracheal injury could cause intratracheal scar hyperplasia which in turn causes benign tracheal stenosis (TS). With the increasing use of mechanical ventilation and ventilator, the incidence of TS is increasing. However, the molecular mechanisms of TS have not been elucidated. It is significant to further explore the molecular mechanisms of TS. Methods The repeatability of public data was verified. Differently expressed genes (DEGs) and most significant genes were identified between TS and normal samples. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed. The comparative toxicogenomics database were analyzed. TS patients were recruited and RT‐qPCR were performed to verify the most significant genes. Results There exist strong correlations among samples of TS and normal group. There was a total of 194 DEGs, including 61 downregulated DEGs and 133 upregulated DEGs. GO were significantly enriched in mitotic nuclear division, cell cycle, and cell division. Analysis of KEGG indicated that the top pathways were cell cycle, and p53 pathway. MKI67(OMIM:176741), CCNB1(OMIM:123836), and CCNB2(OMIM:602755) were identified as the most significant genes of TS, and validated by the clinical samples. Conclusion Bioinformatics methods might be useful method to explore the mechanisms of TS. In addition, MKI67, CCNB1, and CCNB2 might be the most significant genes of TS.

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“… 10 Moreover, it is a predictive biomarker involved in breast cancer diagnosis. 11 Different studies have reported the clinical and prognostic roles of Ki-67 in different types of cancers such as gastric cancer 12 and breast cancer. 13 Besides, Ki-67 expression can be mediated by specific miRNA–mRNA interactions.…”

Section: Introductionmentioning

confidence: 99%

<p>MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression</p>

Ma¹,

Liu²,

Xu³

et al. 2020

OTT

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Background MiR-519d and miR-328-3p had tumor-regulatory properties in different cancers, but their combinatorial effects and potential common target in breast cancer had not been fully reported. This research targeted to study the underlying mechanism of how miR-519d and miR-328-3p cooperatively suppressed breast cancer. Methods MiR-519d and miR-328-3p expressions in breast cancer tissues and cells were assessed and Ki-67 expression was also checked. DLR assay was executed to verify whether Ki-67 was a common target of miR-519d and miR-328-3p. Western blot, flow cytometry, colony formation, wound healing and transwell assays were applied to examine the inhibitory roles of these two miRNAs on the malignant behaviors of breast cancer cells and the potential molecular mechanism. Results Impeded miR-519d and miR-328-3p expressions and enhanced Ki-67 expression were detected in breast cancer tissues and cells. Ki-67 was confirmed as a target of these two miRNAs. MiR-519d and miR-328-3p hampered cell proliferation and blocked cell cycle via binding to Ki-67 and they also suppressed migration and invasion. The combinatorial effects of two miRNAs were much stronger than a single miRNA. Conclusion Our findings proved that miR-519d and miR-328-3p played combinatorial anti-cancer roles in breast cancer by directly targeting a common target Ki-67. Our study suggested that these two miRNAs might own the potential to become novel therapeutic biomarkers involved in the diagnosis and therapy of breast cancer.

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Ki-67/MKI67 as a Predictive Biomarker for Clinical Outcome in Gastric Cancer Patients: an Updated Meta-analysis and Systematic Review involving 53 Studies and 7078 Patients

Cited by 38 publications

References 63 publications

CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis

CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis

Bioinformatics analysis and verification of gene targets for benign tracheal stenosis

<p>MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression</p>

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