Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons

Perez, María Florencia; Isas, Ana Sofía; Aladdin, Azzam; Enshasy, Hesham Ali El; Dib, Julián Rafael

doi:10.1007/978-981-10-5062-6_7

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…These yeasts have already been isolated from natural habitats such as soil, mangroves, and fruits and fermented products such as grape must, kefir, and dairy products [90]. Studies carried out by Perez et al [94] showed that Kazachstania exigua has inhibitory activity against the pathogenic bacteria Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae. Kazachstania telluris species are glucose and lactic acid fermenters; however, they can be considered pathogenic because they cause infections in animals [93,95,96].…”

Section: Discussionmentioning

confidence: 99%

Yeast Diversity in Honey and Pollen Samples from Stingless Bees in the State of Bahia, Brazil: Use of the MALDI-TOF MS/Genbank Proteomic Technique

da Silva,

Magalhães-Guedes,

de Oliveira Alves

et al. 2024

Microorganisms

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(1) Background: The identification of microorganisms includes traditional biochemical methods, molecular biology methods evaluating the conserved regions of rRNA, and the molecular biology of proteins (proteomics), such as MALDI-TOF MS mass spectrometry. This work aimed to identify the biodiversity of yeasts associated with stingless bee species’ honey and pollen, Melipona scutellaris, Nannotrigona testaceicornes, and Tetragonisca angustula, from the region of São Gonçalo dos Campos-Bahia (BA) state, Brazil. (2) Methods: Cellular proteins were extracted from 2837 microbial isolates (pollen and honey) and identified via MALDI-TOF MS. The identified yeast species were also compared to the mass spectra of taxonomically well-characterized reference strains, available from the National Center of Biotechnology Information (NCBI) database. (3) Results: Nine yeast species were identified: Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Brettanomyces bruxellensis, Kazachstania exigua, and Starmerella lactis-condensi. Nannotrigona testaceicornes pollen had the highest number of yeast colonies. The yeasts Brettanomyces bruxellensis and Kazachstania telluris showed high populations in the samples of Nannotrigona testaceicornes and Melipona scutellaris, respectively. This work shows that there is some sharing of the same species of yeast between honey and pollen from the same beehive. (4) Conclusions: A total of 71.84% of the identified species present a high level of confidence at the species level. Eight yeast species (Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Kazachstania exigua, and Starmerella lactis-condensi) were found for the first time in the samples that the authors inspected. This contributes to the construction of new knowledge about the diversity of yeasts associated with stingless bee products, as well as to the possibility of the biotechnological application of some yeast species.

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Section: Discussionmentioning

confidence: 99%

Yeast Diversity in Honey and Pollen Samples from Stingless Bees in the State of Bahia, Brazil: Use of the MALDI-TOF MS/Genbank Proteomic Technique

da Silva,

Magalhães-Guedes,

de Oliveira Alves

et al. 2024

Microorganisms

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Nanotechnology Applied to the Management of Fungal Diseases in Tropical Crops

Pabon-Mora

Quevedo

Ortega‐Toro

2023

Green-Based Nanocomposite Materials and Applications

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Antifungal Mechanism of Rhodotorula mucilaginosa and Aureobasidium sp. nov. Isolated from Cerbera manghas L. against the Growth of Destructive Molds in Post Harvested Apples

Sukmawati

Shabrina

Indrayanti

et al. 2020

FNA

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Background: Apples often experience postharvest damage due to being attacked by mold organisms. Several groups of molds such as Aspergillus sp., Penicilium expansum, Botrytis cinerea, and Venturia sp. can cause a serious postharvest disease exhibited as watery regions where areas of blue-green tufts of spores develop. Current methods using fungicides to control pathogenic fungi can cause resistance if applied in long term. An alternative procedure using yeast as a biological agent has been found. Objective: The aim of this study is to screen potential yeast which has ability to inhibit the growth of Aspergillus brasielensis (isolate A1) and Aspergillus flavus section flavi (isolate A17) isolated from apple fruits. Methods: Antagonism test using YMA dual culture medium using in vitro assays and ITS rDNA identification were performed. Results: The result showed that 3 out of 19 yeast isolated from Cerbera manghas L, T1, T3 and T4, demonstrated the potential ability as biocontrol agent. ITS rDNA identification demonstrated that T1 has similarity to Rhodotorula mucilaginosa while T3 and T4 were identified as Aureobasidium sp. nov.. The 3 isolates exhibited the ability to reduce the growth of A. brasiliensis sensu lato better than dithane 0.3% with a disease incidence (DI) of 100% and a disease severity (DS) value of 45%. Only isolate T1 and T3 were able to reduce decay symptoms in apples inoculated with A. flavus sensu lato (with DO and DS were 100% and 25%, respectively) compared to dithane pesticides 0.3%. Conclusion: This study indicated that competition between nutrients occurs between pathogenic molds and under-yeast in vitro and in vivo conditions. However, further studies in the future might be able to elucidate the 'killer' activity and interaction with the pathogen cells and the bio-product production using Rhodotorula mucilaginosa and Aureoubasidium namibiae strains to control postharvest diseases.

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Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons

Cited by 3 publications

References 57 publications

Yeast Diversity in Honey and Pollen Samples from Stingless Bees in the State of Bahia, Brazil: Use of the MALDI-TOF MS/Genbank Proteomic Technique

Yeast Diversity in Honey and Pollen Samples from Stingless Bees in the State of Bahia, Brazil: Use of the MALDI-TOF MS/Genbank Proteomic Technique

Nanotechnology Applied to the Management of Fungal Diseases in Tropical Crops

Antifungal Mechanism of Rhodotorula mucilaginosa and Aureobasidium sp. nov. Isolated from Cerbera manghas L. against the Growth of Destructive Molds in Post Harvested Apples

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