Kindling Susceptibility and Genetic Seizure Predisposition in Inbred Mice

Green, Robert C.; Seyfried, Thomas N.

doi:10.1111/j.1528-1157.1991.tb05605.x

Cited by 27 publications

(12 citation statements)

References 28 publications

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“…We found that the mean litter size, 3.3±1.4, of Coil −/− mating pairs was significantly smaller than that of wild-type mating pairs, 6.9±2.2 (Figure 1A). Importantly, the average litter size of the wild-type C57BL6/J mating pairs was in good agreement with published data for C57BL6/J mice [36]. Thus, coilin knockout females have fewer pups per litter than do their wild-type counterparts.…”

Section: Resultssupporting

confidence: 88%

Reduced Viability, Fertility and Fecundity in Mice Lacking the Cajal Body Marker Protein, Coilin

2009

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BackgroundCoilin is the signature protein of the Cajal body, a conserved nuclear organelle involved in multiple aspects of small ribonucleoprotein (RNP) biogenesis. Coilin is required for Cajal body homeostasis in both plants and animals. Mice lacking coilin are viable when the mutation is crossed to an outbred strain but only partially viable when crossed to inbred lines.Methodology/Principal FindingsIn order to clarify this issue, we backcrossed the coilin deletion onto the C57BL6/J background for ten generations and then investigated the consequences of coilin removal on overall viability and reproductive success. We conclude that semi-lethal phenotype observed in mixed-background crosses is due to loss of the Coilin gene (or a very tightly-linked locus). Interestingly, coilin knockout embryos die relatively late in gestation, between E13.5 and birth. We show that the maternal contribution of coilin is not important for organismal viability. Importantly, coilin knockout mice display significant fertility and fecundity defects. Mutant males that escape the embryonic lethality display reduced testis size, however, both males and females contribute to the observed reduction in reproductive fitness.Conclusions/SignificanceThe evolutionary conservation of coilin from plants to animals suggests that the protein plays an important role, perhaps coordinating the activities of various RNA-processing machineries. Our observations are consistent with the idea that coilin functions to ensure robust organismal development, especially during periods of rapid growth.

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Section: Resultssupporting

confidence: 88%

Reduced Viability, Fertility and Fecundity in Mice Lacking the Cajal Body Marker Protein, Coilin

2009

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“…By means of CCEP, we demonstrated a human cortico-cortical network connecting 1) anatomical homologous areas of the lateral and medial motor cortex along the rostro-caudal cognitive-motor gradient [37–39] (e.g., supplementary motor area (SMA) to the caudal lateral premotor/primary motor (MI) area, pre-SMA to the rostral lateral premotor area), and 2) the somatotopically homologous regions in the lateral and medial motor cortices (e.g., the face SMA to the precentral hand face area) in a reciprocal manner [20]. Clinically, these circuits could account for the propagation of epileptic discharge from/into SMA [40] and the atypical motor responses infrequently seen in standard cortical stimulation: tonic and clonic responses in MI and SMA stimulation, respectively [41]. Functionally, it was not only the dorsal premotor area (mainly the caudal superior frontal gyrus and adjacent precentral gyrus) but also the ventral premotor area (mainly the caudal inferior frontal gyrus and adjacent precentral gyrus) that connected to the pre-SMA and SMA.…”

Section: Ccep Methodologymentioning

confidence: 99%

Single pulse electrical stimulation to probe functional and pathological connectivity in epilepsy

Matsumoto

Kunieda

Nair

2017

Seizure

151

160

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In the last decade, single pulse electrical stimulation (SPES) has been used as an investigational tool in the field of epilepsy surgery. Direct cortical stimulation applied at a frequency of ∼1 Hz can probe cortico-cortical connections by averaging electrocorticogram time-lock to the stimuli (2 x 20–30 trials). These evoked potentials that emanate from adjacent and remote cortices have been termed cortico-cortical evoked potentials (CCEPs). Although limited to patients undergoing invasive presurgical evaluations with intracranial electrodes, CCEP provides a novel way to explore inter-areal connectivity in vivo in the living human brain to probe functional brain networks such as language and cognitive motor networks. In addition to its impact on systems neuroscience, this method, in combination with 50 Hz electrical cortical stimulation, could contribute clinically to map the functional brain systems by tracking the cortico-cortical connections among the functional cortical regions in each individual patient. This approach may help identify the normal cortico-cortical network within pathology as well as reveal connections that might arise from neural plasticity. Because of its high practicality, it has been recently applied for intraoperative monitoring of the functional brain networks for patients with brain tumor. With regard to epilepsy, SPES has been used for the two major purposes, one to probe cortical excitability of the focus, namely, epileptogenicity, and the other to probe seizure networks. Both early (i.e., CCEP) and delayed responses, and probably their high frequency oscillation counterparts, are regarded as a surrogate marker of epileptogenicity. With regards to its impact on the human brain connectivity map, worldwide collaboration is warranted to establish the standardized CCEP connectivity map as a solid reference for non-invasive connectome researches.

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“…In a subsequent study [113], olfactory bulb kindling rates were studied in two inbred strains of genetically seizure-prone mice (DBA/2 and EL) and in three non-epileptic inbred strains (B6, ddY, and C3H/He). The seizure sensitive DBA/2 strain required the fewest number of stimulations to reach stage 5 seizures, whereas C3H/He mice required the most stimulations to reach stage 5 seizures.…”

Section: Interstrain Differences In the Expression Of Seizures Andmentioning

confidence: 99%

The relevance of inter- and intrastrain differences in mice and rats and their implications for models of seizures and epilepsy

Löscher¹,

Ferland

Ferraro

2017

Epilepsy & Behavior

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It is becoming increasingly clear that the genetic background of mice and rats, even in inbred strains, can have a profound influence on measures of seizure susceptibility and epilepsy. These differences can be capitalized upon through genetic mapping studies to reveal genes important for seizures and epilepsy. However, strain background and particularly mixed genetic backgrounds of transgenic animals need careful consideration in both the selection of strains and in the interpretation of results and conclusions. For instance, mice with targeted deletions of genes involved in epilepsy can have profoundly disparate phenotypes depending on the background strain. In this review, we discuss findings related to how this genetic heterogeneity has and can be utilized in the epilepsy field to reveal novel insights into seizures and epilepsy. Moreover, we discuss how caution is needed in regards to rodent strain or even animal vendor choice, and how this can significantly influence seizure and epilepsy parameters in unexpected ways. This is particularly critical in decisions regarding the strain of choice used in generating mice with targeted deletions of genes. Finally, we discuss the role of environment (at vendor and/or laboratory) and epigenetic factors for inter- and intrastrain differences and how such differences can affect the expression of seizures and the animals’ performance in behavioral tests that often accompany acute and chronic seizure testing.

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Kindling Susceptibility and Genetic Seizure Predisposition in Inbred Mice

Cited by 27 publications

References 28 publications

Reduced Viability, Fertility and Fecundity in Mice Lacking the Cajal Body Marker Protein, Coilin

Reduced Viability, Fertility and Fecundity in Mice Lacking the Cajal Body Marker Protein, Coilin

Single pulse electrical stimulation to probe functional and pathological connectivity in epilepsy

The relevance of inter- and intrastrain differences in mice and rats and their implications for models of seizures and epilepsy

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