Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1

Oshiro, Mugihito; Shinto, Hideaki; Tashiro, Yukihiro; Miwa, Noriko; Sekiguchi, Tatsuya; Okamoto, Masahiro; Ishizaki, Ayaaki; Sonomoto, Kenji

doi:10.1016/j.jbiosc.2009.05.003

Cited by 28 publications

(17 citation statements)

References 27 publications

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“…This value is very close to the maximum theoretical yield obtained through the PP/glycolytic pathway (1.67 mol/mol) and is comparable to the 1.48-mol/mol yield of lactate produced via only the PP/ glycolytic pathway by PK gene-disrupted L. plantarum mutant in homolactate fermentation with xylose (12). In addition, very small amounts of formic acid, acetic acid, and ethanol were produced during xylose fermentation by the QU 25 strain, which are probably formed by pyruvate formate lyase or pyruvate dehydrogenase, phosphotransacetylase/acetate kinase, and aldehyde and alcohol dehydrogenases, respectively, as previously reported (14,20). Note that these low levels of byproducts would probably result in lower purification cost (7).…”

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confidence: 78%

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Efficient Homofermentative l -(+)-Lactic Acid Production from Xylose by a Novel Lactic Acid Bacterium, Enterococcus mundtii QU 25

Abdel-Rahman

Tashiro

Zendo

et al. 2011

Appl Environ Microbiol

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confidence: 78%

“…Few LAB strains produce lactate from xylose, the major constituent of hemicelluloses in lignocellulosic biomass and the second most abundant sugar, next to glucose, in nature (14). The phosphoketolase (PK) pathway in LAB converts 2 of the 5 carbons in xylose to acetic acid, increasing the purification cost of lactic acid (6,16,20).…”

mentioning

confidence: 99%

Efficient Homofermentative l -(+)-Lactic Acid Production from Xylose by a Novel Lactic Acid Bacterium, Enterococcus mundtii QU 25

Abdel-Rahman

Tashiro

Zendo

et al. 2011

Appl Environ Microbiol

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“…Lactic acid (2-hydroxypropanoic acid, CH 3 -CH(OH)-COOH) is a natural organic acid with a long history of use in the food and non-food industries, including the cosmetic and pharmaceutical industries, and for the production of oxygenated chemicals, plant growth regulators, and special chemical intermediates [1][2][3]. Currently, there is an increased demand for lactic acid as a feedstock for the production of biopolymer poly-lactic acid (PLA), which is a promising biodegradable, biocompatible, and environmentally friendly alternative to plastics derived from petrochemicals.…”

Section: Introductionmentioning

confidence: 99%

Isolation of lactic acid bacteria from sugar cane juice and production of lactic acid from selected improved strains

Sobrun¹,

Bhaw‐Luximon²,

Jhurry³

et al. 2012

ABB

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Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product

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“…Recently, some strains capable of utilizing xylose [19-21] and cellobiose [10,22-24] for lactic acid fermentation were reported. Not even that, Okano et al [25] reported the directed lactic acid fermentation from β-glucan and a cellooligosaccharide by introducing an endoglucanase from a C. thermocellum into L. plantarum Δ ldhL1 .…”

Section: Resultsmentioning

confidence: 99%

Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

Yang

Lai

et al. 2013

Biotechnol Biofuels

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BackgroundEfficient conversion of lignocellulosic biomass to optically pure lactic acid is a key challenge for the economical production of biodegradable poly-lactic acid. A recently isolated strain, Thermoanaerobacterium aotearoense SCUT27, is promising as an efficient lactic acid production bacterium from biomass due to its broad substrate specificity. Additionally, its strictly anaerobic and thermophilic characteristics suppress contamination from other microoragnisms. Herein, we report the significant improvements of concentration and yield in lactic acid production from various lignocellulosic derived sugars, achieved by the carbon flux redirection through homologous recombination in T. aotearoense SCUT27.ResultsT. aotearoense SCUT27 was engineered to block the acetic acid formation pathway to improve the lactic acid production. The genetic manipulation resulted in 1.8 and 2.1 fold increase of the lactic acid yield using 10 g/L of glucose or 10 g/L of xylose as substrate, respectively. The maximum l-lactic acid yield of 0.93 g/g glucose with an optical purity of 99.3% was obtained by the engineered strain, designated as LA1002, from 50 g/L of substrate, which is very close to the theoretical value (1.0 g/g of glucose). In particular, LA1002 produced lactic acid at an unprecedented concentration up to 3.20 g/L using 10 g/L xylan as the single substrate without any pretreatment after 48 h fermentation. The non-sterilized fermentative production of l-lactic acid was also carried out, achieving values of 44.89 g/L and 0.89 g/g mixed sugar for lactic acid concentration and yield, respectively.ConclusionsBlocking acetic acid formation pathway in T. aotearoense SCUT27 increased l-lactic acid production and yield dramatically. To our best knowledge, this is the best performance of fermentation on lactic acid production using xylan as the sole carbon source, considering the final concentration, yield and fermentation time. In addition, it should be mentioned that the performance of non-sterilized simultaneous fermentation from glucose and xylose was very close to that of normal sterilized cultivation. All these results used the mutant strain, LA1002, indicated that it is a new promising candidate for the effective production of optically pure l-lactic acid from lignocellulosic biomass.

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Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1

Cited by 28 publications

References 27 publications

Efficient Homofermentative l -(+)-Lactic Acid Production from Xylose by a Novel Lactic Acid Bacterium, Enterococcus mundtii QU 25

Efficient Homofermentative l -(+)-Lactic Acid Production from Xylose by a Novel Lactic Acid Bacterium, Enterococcus mundtii QU 25

Isolation of lactic acid bacteria from sugar cane juice and production of lactic acid from selected improved strains

Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

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