Kinetics and tissue distribution of neutron-activated zinc oxide nanoparticles and zinc nitrate in mice: effects of size and particulate nature

Yeh, Teng‐Kuang; Chen, Jen‐Kun; Lin, Chia‐Hua; Yang, Mo‐Hsiung; Yang, Chu-Sing; Chou, Fong‐In; Peir, Jinn-Jer; Wang, Mei-Ya; Chang, Wan-Hsuan; Tsai, Ming-Hsien; Tsai, Hui-Ti; Lin, Pinpin

doi:10.1088/0957-4484/23/8/085102

Cited by 40 publications

(36 citation statements)

References 25 publications

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“…32 In this regard, ZnO NPs would encounter diverse immune cells in the human body, thus damaging immune tissues and organs. However, the immunotoxicity of ZnO NPs in relation to the size and charge of ZnO NPs is unclear.…”

Section: Discussionmentioning

confidence: 99%

Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge

Kim

Nguyen

Ignacio

et al. 2014

IJN

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While zinc oxide (ZnO) nanoparticles (NPs) have been recognized to have promising applications in biomedicine, their immunotoxicity has been inconsistent and even contradictory. To address this issue, we investigated whether ZnO NPs with different size (20 or 100 nm) and electrostatic charge (positive or negative) would cause immunotoxicity in vitro and in vivo, and explored their underlying molecular mechanism. Using Raw 264.7 cell line, we examined the immunotoxicity mechanism of ZnO NPs as cell viability. We found that in a cell viability assay, ZnO NPs with different size and charge could induce differential cytotoxicity to Raw 264.7 cells. Specifically, the positively charged ZnO NPs exerted higher cytotoxicity than the negatively charged ones. Next, to gauge systemic immunotoxicity, we assessed immune responses of C57BL/6 mice after oral administration of 750 mg/kg/day dose of ZnO NPs for 2 weeks. In parallel, ZnO NPs did not alter the cell-mediated immune response in mice but suppressed innate immunity such as natural killer cell activity. The CD4 + /CD8 + ratio, a marker for matured T-cells was slightly reduced, which implies the alteration of immune status induced by ZnO NPs. Accordingly, nitric oxide production from splenocyte culture supernatant in ZnO NP-fed mice was lower than control. Consistently, serum levels of pro/anti-inflammatory (interleukin [IL]-1β, tumor necrosis factor-α, and IL-10) and T helper-1 cytokines (interferon-γ and IL-12p70) in ZnO NP-fed mice were significantly suppressed. Collectively, our results indicate that different sized and charged ZnO NPs would cause in vitro and in vivo immunotoxicity, of which nature is an immunosuppression.

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Section: Discussionmentioning

confidence: 99%

Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge

Kim

Nguyen

Ignacio

et al. 2014

IJN

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“…15 Various studies have shown that ZnO NPs with various shapes, such as rods and spheres, probably cause neurotoxicity due to their ability to access the brain. [16][17][18] Moreover, the olfactory brain route has also been reported as a potential route for ZnO NPs transfer into the brain. 19 However, information regarding the neurotoxic effects of ZnO NPs is scarce.…”

Section: Introductionmentioning

confidence: 99%

Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner

Liu

Kang

Yin

et al. 2017

IJN

108

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Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnO a NPs and ZnO b NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnO b NPs induced more significant toxic responses than ZnO a NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability.

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“…The kinetics of neutron-activated 65 ZnO nanoparticles of two different sizes (10 and 71 nm) was evaluated in mice after intravenous injection, with 65 ZnNO 3 as a control; 30 the kinetic parameters for the two nanoparticle sizes were similar, but greater plasma AUC and t ½ values were found for 65 ZnNO 3 than 65 ZnO nanoparticles. These results are in good agreement with the report by Paek et al, described earlier.…”

mentioning

confidence: 99%

Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction

Choi

Choy

2014

IJN

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Biokinetic studies of zinc oxide (ZnO) nanoparticles involve systematic and quantitative analyses of absorption, distribution, metabolism, and excretion in plasma and tissues of whole animals after exposure. A full understanding of the biokinetics provides basic information about nanoparticle entry into systemic circulation, target organs of accumulation and toxicity, and elimination time, which is important for predicting the long-term toxic potential of nanoparticles. Biokinetic behaviors can be dependent on physicochemical properties, dissolution property in biological fluids, and nanoparticle–protein interaction. Moreover, the determination of biological fates of ZnO nanoparticles in the systemic circulation and tissues is critical in interpreting biokinetic behaviors and predicting toxicity potential as well as mechanism. This review focuses on physicochemical factors affecting the biokinetics of ZnO nanoparticles, in concert with understanding bioavailable fates and their interaction with proteins.

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Kinetics and tissue distribution of neutron-activated zinc oxide nanoparticles and zinc nitrate in mice: effects of size and particulate nature

Cited by 40 publications

References 25 publications

Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge

Immunotoxicity of zinc oxide nanoparticles with different size and electrostatic charge

Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner

Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction

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