Kinetics of Cellular Component in Inflammatory Response Induced by Different Stimuli in the Swim Bladder of Pacu, Piaractus mesopotamicus Holmberg 1887 (Characidae)

Abstract

Abstract.-The present study assessed the kinetics of cell accumulation at the site of inflammation induced by thioglycolate, Escherichia coli lipopolysaccharide (LPS) and heat-inactivated Aeromonas hydrophila, in the pacu, Piaractus mesopotamicus (Characidae), swim bladder. A quantitative, as well as qualitative, assessment was done of all the cells present in the exudate at 6, 24, and 48 h (n 5 8) after inoculation of inflammatory agents. The results show that the thioglycolate was the

Haematological changes in Nile tilapia experimentally infected with Enterococcus sp.

“…In fact, whether thrombocytes are involved in fish cell response, these results could suggest their participation in such event. This is especially true in analyzing the findings with tilapia inflammation (Martins et al, 2004b;Bozzo et al, 2007). In this previous study, thrombocytes were the main cells present in the inflammatory exsudate suggesting their importance in fish cell response.…”

“…It must be commented that the injection in the swim bladder could not be as aggressive to the same degree of intraperitoneal injection. Previous study showed the security in the use of the swim bladder as an inflammatory site (Martins et al, 2004a, Bozzo et al, 2007 by dint of the fact that this organ suffers little influence of contamination.…”

“…Nowadays, thrombocyte has been found to be an important cell involved in fish defenses (Matushima and Mariano, 1996;Martins et al, 2006;Tavares-Dias and Moraes, 2007;Bozzo et al, 2007). Besides the high number of lymphocytes, the injection of Enterococcus was found to incite more production of thrombocytes in the circulating blood.…”

Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

“…In fish, the assessment of inflammatory response depends on three major factors: first is the difficulty of identification of leukocytes, that depends on the species, the second is related to ontogenetic variation in blood and inflammatory cells (Tavares- Dias and Moraes, 2004), and third kinetics of inflammatory process, which may be influenced by dietary supplementation (Moraes et al, 2003;Belo et al, 2005Belo et al, , 2012Dotta et al, 2011), by the kind of inflammatory stimulous (Bozzo et al, 2007), by eicosanoids (Claudiano, 2011), by the ambiental temperature (Dias and Sinhorini, 1991;Jansson Junior and Waaler, 1967) and by circulating hormones (Martins et al, 2000;Belo et al, 2005Belo et al, , 2012.…”

“…In fish, carrageenin, thioglycolate and inactivated Aeromonas hydrophila were tested in the swim bladder of Piaractus mesopotamicus (Martins et al, 2000;Moraes et al, 2003;Bozzo et al, 2007) and Oreochromis niloticus (Martins et al, 2004;Dotta et al, 2011). According to Bozzo et al (2007), thioglycolate and inactivated Aeromonas hydrophila induced vascular congestion, accumulation of thrombocytes followed by macrophages, granulocytes and oedema. The choice of swim bladder as local for assessment of inflammatory response is based on studies of Van Furth (1992), which showed that body cavities are an experimental model to represent the mechanisms of inflammatory response that can occur in the other animal tissues.…”