Kinetics of epoxidation by a Musa paradisiaca chloroperoxidase

Abstract

Epoxidations of indene, styrene, 2-chlorostyrene, 3-chlorostyrene, 4-chlorostyrene, 4-bromostyrene, and naphthalene using Musa paradisiaca plant juice chloroperoxidase in the presence of H 2 O 2 and t-butyl peroxide as oxidants have been studied. The steady-state kinetic parameters, K m and k cat of the enzyme for the above substrates have been determined. The temperature and pH optima of the epoxidation are 25 • C and 6.2, respectively. The yield of styrene oxide in the presence of H 2 O 2 was 44%. The results show that M. paradisiaca plant juice chloroperoxidase is a potential biocatalyst for organic epoxidation reactions. K E Y W O R D Sbiocatalyst, chloroperoxidase, epoxidation, kinetic parameters, Musa paradisiaca 602