Kinetics of quiescent cord blood stem/progenitor cells with high proliferative potential in stem-cell expansion culture

Goussetis, Evgenios; Theodosaki, Maria; Paterakis, George; Stefanaki, Katerina; Tsecoura, Christina; Papassarandis, C.; Graphakos, Stelios

doi:10.1080/14653240310003602

Cited by 5 publications

(2 citation statements)

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“…Though quantitatively limited, the proportion of highly proliferative HSCs is greater in UCB as compared to BM or PB allografts and has an enhanced capacity for homing and hematopoietic reconstitution ( 17 , 18 ). These UCB cells also have higher capability for self-renewal, proliferation, and expansion under optimal conditions ( 19 , 20 ). Higher expression of CD34+ antigen on their cell surface along with longer telomere length is an advantage.…”

Section: Hematopoietic Propertiesmentioning

confidence: 99%

Umbilical Cord Blood Transplants: Current Status and Evolving Therapies

Gupta

Wagner

2020

Front. Pediatr.

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Hematopoietic cell transplants using stem cells from umbilical cord blood are used worldwide for the treatment of malignant and non-malignant disorders. Transplant procedures from this stem cell source have shown promising outcomes in successfully treating various hematologic, immunologic, malignant, and inherited metabolic disorders. Rapid availability of these stem cells is an important advantage over other unrelated donor transplants, especially in situations where waiting can adversely affect the prognosis. The umbilical cord blood is rich in CD34+ stem cells, though with a limited cell dose and usually takes longer to engraft. Limitations around this have been addressed by in vivo and ex vivo expansion techniques as well as enhanced engraftment kinetics. Development of adoptive immunotherapy using other components of umbilical cord blood such as regulatory T cells, virus-specific T cells, and natural killer cells has further transformed the field and enhanced the utility of umbilical cord blood unit.

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Section: Hematopoietic Propertiesmentioning

confidence: 99%

Umbilical Cord Blood Transplants: Current Status and Evolving Therapies

Gupta

Wagner

2020

Front. Pediatr.

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“…The presence of such a cell type is very important for long-term hematopoiesis reconstitution. The distribution of the CD38-population was measured during the isolation of individual populations as well as during short-term cultivation [6].…”

Section: Introductionmentioning

confidence: 99%

Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood

Koutná¹,

Peterková²,

Šimara³

et al. 2010

Ann Hematol

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of CD133+ and CD34+ populations from bone marrow and mobilized peripheral blood. Patients and Methods:Eight cell populations were compared: CD34+ and CD133+ cells from both the BM (CML Ph-, CML Ph+ and healthy volunteers) and mobilized peripheral blood cells. Multicolor flow cytometry and cultivation experiments were used to measure expression and differentiation of the individual populations.Results: It was observed that the CD133+ BM population showed higher cell expansion.Another finding is that during a six-day cultivation with CFSE, more cells remained in division D0 (non-dividing cells). There was a higher percentage of CD38-cells observed on the CD133+ BM population. It was also observed that the studied populations contained very similar but not the same pools of progenitors: erythroid, lymphoid, and myeloid. This was confirmed by CFU-GM and CFU-E experiments.The VEGFR antigen was used to monitor subpopulations of endothelial sinusoidal progenitors. The CD133+ BM population contained significantly more VEGFR+ cells. Conclusion:Our findings suggest that the CD133+ population from the BM shows better proliferation activity and a higher distribution of primitive progenitors than any other studied population.

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