Kinetics of Sequential Protopectin Degradation in a Flowing Reaction Solution

Barofractionation as an Innovative Method to Obtain Pectic Polysaccharides

“…The mechanism of the degradation of protopectin was previously shown [22,23]. To increase the percentage of the yield of oligosaccharides, it is necessary to accelerate the process of degradation of protopectin, which proceeds according to a previously proven mechanism.…”

“…It has been experimentally established that the optimum pH value of the hydrolyzing agent is 2.0. And also, in comparison with the method of obtaining PPs in a static mode, the duration of the hydrolysis-extraction process is significantly reduced from 60 to 3-10 minutes [22,23].…”

“…Dried and crushed secondary pectin-containing phytomass of sunflower head (SH) was used as raw material. Pectic polysaccharides were obtained by methods of extraction in static mode (SM) [22], baroextraction (Be) [22], combined fractionation (CFr) [23] and the developed method of barofractionation. The resulting hydrolysis solutions were neutralized to pH = 3.5, centrifuged at a speed of 4000-5000 rpm to separate the pectin microgel.…”

“…The resulting hydrolysis solutions were neutralized to pH = 3.5, centrifuged at a speed of 4000-5000 rpm to separate the pectin microgel. Pectic substances were isolated from the remaining mother liquor by precipitation with ethanol (1: 3) [22,23]. To obtain pectic oligosaccharides, the residual solution was evaporated to an oily state and treated with alcohol, followed by precipitation with ether in a 2: 1 ratio.…”

“…The resulting powder was separated from the solution using a Schott filter and dried at room temperature. The molecular weight (MW) of the polymers was determined by HPLC [22,23].…”