Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats

Aliabadi, Elham; Namavar, Mohammad Reza; Mortezaee, Keywan; Toolee, Heidar; Keshtgar, Sara; Mirkhani, Hossein; Akbari, Mohammad; Rastegar, Tayebeh; Solhjoo, Somayeh

doi:10.1007/s00404-017-4509-3

Cited by 24 publications

(19 citation statements)

References 19 publications

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“…Moreover, we found that the expression of Kiss1 mRNA and immunoreactivity were significantly increased in the ARC of both models. These results are similar to those showing an increase of Kiss1 neurons in the ARC of progesterone antagonist RU486‐induced (8) and letrozole‐induced PCOS rats (9) but are in opposition to those showing a decrease of Kiss1 neurons in the ARC of dihydrotestosterone‐induced PCOS rats (7). These findings provide solid evidence indicating possible involvement of Kiss1 neurons in the hypothalamic ARC during the development of PCOS.…”

Section: Discussionsupporting

confidence: 84%

“…These findings provide solid evidence indicating possible involvement of Kiss1 neurons in the hypothalamic ARC during the development of PCOS. The discrepancy observed in these independent investigations might be due to differences in the experimental approaches (e.g., rat genetic background, ages, induction drugs, and even antibodies with various specificities) (7‐9). Thus, additional data are required for clarifying the mechanism underlying PCOS development in these models.…”

Section: Discussionmentioning

confidence: 99%

“…Kiss1 neurons in the ARC are considered to participate in negative feedback, whereas the Kiss1 neurons in the AVPV appear to play a role in mediating the positive feedback effects of estradiol (E 2 ) (5). Other studies (7‐9) indicate that Kiss1 may be involved in the disturbance of the hypothalamic‐pituitary‐ovarian axis in PCOS rat models.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome

Wen

Lin

et al. 2020

Obesity

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Objectives: The purpose of this study was to determine changes in the expression levels of kisspeptin-1 (Kiss1) in the hypothalamus during the development of polycystic ovary syndrome (PCOS) and after treatment with sleeve gastrectomy (SG). Methods: This study used chronic dehydroepiandrosterone (DHEA) alone and DHEA plus a high-fat diet (HFD) to generate a PCOS rat model. Subsequently, SG was performed in the animals with PCOS and the effects on glucose tolerance, insulin sensitivity, sex hormones, estrous cyclicity, adiponectin, and Kiss1 expression in the hypothalamus were investigated. Results: Impaired glucose tolerance, decreased insulin sensitivity, reduced adiponectin levels, disrupted estrous cyclicity, and elevated sex hormone levels associated with PCOS models were restored to normal following SG. In addition, SG was able to restore the increase in the expression of Kiss1 mRNA and Kiss1-positive neurons in the arcuate nucleus of rats with PCOS. Interestingly, although SG did not result in a significant loss of body weight in rats administered DHEA under a chow diet, it resulted in comparable metabolic improvements and Kiss1 expression in rats that had been administered DHEA along with an HFD. Conclusions: The recovery of normal levels of Kiss1 expression in the hypothalamus after SG in this study suggests that Kiss1 might play an important role in the development of PCOS and its improvement by SG.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome

Wen

Lin

et al. 2020

Obesity

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“…Recently, a variety of experimental animal studies have been conducted to estimate KISS1, KISS r, and KISSpositive cells in different PCOS-induced phenotypes. Aliabadi et al found that [26,27] kisspeptin-positive cells and kiss mRNA expression in letrozol-injected female rats were higher than normal rats. Additionally, they demonstrated that the enhanced neural cells in PCOS rats might contribute to the hypersecretion of LH.…”

Section: Discussionmentioning

confidence: 99%

The Correlation between Hormonal Disturbance in PCOS Women and Serum Level of Kisspeptin

Ibrahim

Omer

Fattah

2020

International Journal of Endocrinology

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Background. Kisspeptin is a neuropeptide that upregulates gonadotropin-releasing hormone (GnRH) secretion. It is an essential element for the luteinizing hormone (LH) surge and ovulation. Women with polycystic ovary syndrome (PCOS) expose alteration in both GnRH and LH secretion levels. Objective. This paper aims to evaluate serum kisspeptin levels in healthy and polycystic ovarian syndrome women. Furthermore, it investigates the effect of obesity and age on circulating kisspeptin levels in both normal and PCOS women. Moreover, it points out the correlation between kisspeptin and other hormonal parameters. Methods and Patients. One hundred women (60 are with PCOS and 40 are normal) were enrolled in the study. Five milliliter samples of blood from all the patients and control women were obtained twice during the menstrual cycle. All the study samples were classified depending on the age factor for several subgroups. Results. Kisspeptin levels were higher in PCOS patients than those in the normal group. Kisspeptin correlated with serum free testosterone level (r=0.26). In healthy women, preovulatory kisspeptin levels were higher than follicular kisspeptin levels (P<0.05), while this difference was insignificant in PCOS patients. The variation in serum kisspeptin levels between overweight/obese and normal-weight women was insignificant. In normal women, serum kisspeptin levels were higher in women >35 years than those <24 years at (P=0.03). Conclusion. The serum kisspeptin level is higher in PCOS women. Its levels fluctuate during the menstrual cycle, but these fluctuations are disturbed in PCOS women. The effect of BMI on serum kisspeptin levels is insignificant, and kisspeptin serum levels increase with age.

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“…15 Somatic cells exert their effects on SCs by releasing factors like GDNF and EGE2. 26 Many of possible roles for Kp on germ cells via the mentioned network are still elusive. Consistently, Kp-10 has been reported to make alterations in the rate of expression for CX43, the key junctional protein linking germ and Sertoli cells.…”

Section: Introductionmentioning

confidence: 99%

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells

Toolee

Rastegar

Solhjoo

et al. 2018

J of Cellular Biochemistry

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Kisspeptin (Kp) expression in testis has caused most of the recent research surveying its functional role in this organ. This peptide influences spermatogenesis and sperm capacitation, so it is considered as a regulator of reproduction. Kp roles exert through hypothalamic/pituitary/gonadal axis. We aimed to evaluate direct roles for Kp on proliferation and differentiation of spermatogonial cells (SCs) when the cells are cocultured with somatic cells. Somatic cells and SCs were isolated from adult azoospermic and newborn mice and then enriched using a differential attachment technique. After the evaluation of identity and colonization for SCs, the cells were cocultured with somatic cells, and three doses of Kp (10−8‐10−6 M) was assessed on proliferation (through evaluation of MVH and ID4 markers) and differentiation (via evaluation of c‐Kit and SCP3, TP1, TP2, and, Prm1 markers) of the coculture system. Investigations were continued for four succeeding weeks. At the end of each level of testosterone in the culture media was also evaluated. We found positive influence from Kp on proliferative and differentiative markers in SCs cocultured with somatic cells. These effects were dose‐dependent. There was no effect for Kp on testosterone level. From our findings, we simply conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis.

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Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats

Abstract: Our findings show that the number of kisspeptin-positive cells may be affected from letrozole, and that the changes in the number of these cells may be in favor of the appearance of PCOS features in this group.

Cited by 24 publications

References 19 publications

Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome

Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome

The Correlation between Hormonal Disturbance in PCOS Women and Serum Level of Kisspeptin

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells

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