Kit Designing for Assessing Ros and SCD in Semen: Replication of Methodology Who Laboratory Manual (2021) in to Russian Laboratory Practice

Abstract

According to WHO laboratory manual for the examination and processing of human semen, sixth edition (2021) [1], there are a number of techniques available to measure ROS (Reactive Oxygen Species) and DFI levels in semen and each of them has its inherent limitations. According to WHO (2021), two methods for measuring ROS and DFI seems to be attractive due to reliability and simplifying of performance not only in laboratory conditions but also in point-of-care (POC) one. Russian technique utilizes Tetrazolium Nitroblue for measuring ROS which kit named ‘OxySperm (NBT-assay)’ and SCD-assay named ‘HaloSperm’ have been replicated and upgrade methods compared to WHO (2021) and another fundamental scientific papers. Both kits showed not only reproducible results compared with WHO (2021) but also have technological advantages over the previous described methods. This publication presents step-by-step operational procedures of novel Russian kits.