Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen

Abstract

Act d 11 is the first member of the MLP/RRP protein family to be described as an allergen. It displays IgE co-recognition with allergens belonging to the PR-10 family, including Bet v 1.

Analysis of the potential allergenicity of traditional apple cultivars by Multiplex Biochip-Based Immunoassay

“…The analysis of the protein concentration, the profile on SDS-PAGE and the pattern of allergen inhibition on the multiplex microarray system (D'Avino et al, 2011;Scala et al, 2010) revealed that a unique combination of features characterises each of the twelve investigated apple cultivars. Individual patterns of IgG, IgG4 and IgE inhibitions were obtained for the cultivars tested on the available allergens.…”

“…A control sample, where only the buffer solution was added, was used as reference value for no inhibition IgE values. The assay was run as a single point highest inhibition achievable assay (SPHIAa) (D'Avino et al, 2011). The testing protocol was adapted for IgG and IgG4 detection by mixing the additional two secondary antibodies (PMD) with the anti-IgE.…”

Structure and Function of the Peanut Panallergen Ara h 8

“…The PR-10 family and MLP/RRP family both belong to the Bet v 1 superfamily despite low sequence identity (Ͻ25%) between the two protein families (42). D'Avino et al (43) were able to show that Act d 11 is able to inhibit the binding of IgE to Bet v 1, Cor a 1, Dau c 1, and Mal d 1. These results indicate that these allergens, despite being from separate protein families, share some epitopes (43).…”

“…D'Avino et al (43) were able to show that Act d 11 is able to inhibit the binding of IgE to Bet v 1, Cor a 1, Dau c 1, and Mal d 1. These results indicate that these allergens, despite being from separate protein families, share some epitopes (43). Currently, there are seven protein structures of Act d 11 deposited in the PDB (PDB entries 4IGV, 4IGW, 4IGX, 4IGY, 4IH0, 4IH2, and 4IHR).…”

Bioanalytical Methods for Food Allergy Diagnosis, Allergen Detection and New Allergen Discovery

“…The described weaknesses of allergenomic strategies for new allergen identification lead to the necessity in the application of DNA-based techniques [115] or in Edman degradation performance for peptide sequencing [41,58,116,117,118]. It insures unequivocal identification of amino acid order in the peptide starting from the N-terminal residue and is typically realized in parallel with de novo sequencing [82][83][84].…”