Klf7 modulates the differentiation and proliferation of chicken preadipocyte

Zhang, Zhiwei; Wang, Haixia; Sun, Yingning; Li, Hui; Wang, Ning

doi:10.1093/abbs/gmt010

Cited by 63 publications

(67 citation statements)

References 24 publications

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“…14,16,18) Our previous study indicated that one member of KLFs, KLF7, is expressed in chicken adipose tissues, and plays a negative role in the regulation of chicken adipogenesis. 13,19) In the current study, the results showed that KLF3, an another member of KLFs, was also expressed in chicken adipose tissue. In addition, the sequence alignment showed that KLF3 protein is highly conserved among chicken, mouse and human, suggesting that KLF3 may have a similar function in these species (Fig.…”

Section: Discussionsupporting

confidence: 51%

“…DF-1 chicken embryo fibroblast cell line (kindly provided by the Harbin Veterinary Research Institute, Harbin, China) and chicken preadipocytes, which were isolated according to the procedure previously described, 13) were grown in DMEM/F12 medium (Invitrogen), supplemented with 10% fetal calf serum (Invitrogen), and plated in 12-well dishes. In transfection assays, a fixed amount of total DNA (1 μg) was transfected in each well using the Fu-GENE HD transfection reagent (Roche).…”

Section: Klf3 Cloning and Vector Constructionmentioning

confidence: 99%

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Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

Zhang

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Studies in mammalian species showed that Krüppel-like factor 3 (KLF3) regulated adipose tissue development. However, it was not reported in chicken. In the current study, we found that during the growth and development of abdominal fat tissue, chicken KLF3 (Gallus gallus KLF3, gKLF3) was consecutively expressed, and its transcripts were higher at 7 weeks of age and lower at 10 weeks of age in lean broilers than in fat broilers. In addition, gKLF3 overexpression suppressed chicken CCAAT/enhancer binding protein alpha (C/EBPα), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN), and lipoprotein lipase (LPL) promoter activities, but increased chicken peroxisome proliferator-activated receptor gamma (PPARγ) promoter activity. Additionally, point mutagenesis analysis showed that the substitution of Asp by Gly within the Pro-Val-Asp-Leu-Thr (PVDLT) motif of gKLF3 significantly reduced the ability of gKLF3 to regulate the promoter activities of FABP4, FASN, LPL, C/EBPα, and PPARγ.

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Section: Discussionsupporting

confidence: 51%

Section: Klf3 Cloning and Vector Constructionmentioning

confidence: 99%

Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

Zhang

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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“…To date, several studies have shown that PPAR γ and C/EBP α (Yu et al 2014), as well as FATP1 (Qi et al 2013) and Klf7 (Zhang et al 2013) regulate the differentiation of preadipocytes in the chicken. However, to our knowledge, investigations into the roles of lncRNAs in preadipocyte differentiation in the chicken have not yet been conducted.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

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Long noncoding RNAs (lncRNAs) regulate adipogenesis and other processes associated with metabolic tissue development and function. However, little is known about the function and profile of lncRNAs during preadipocyte differentiation in the chicken (Gallus gallus). Herein, lncRNA and mRNA expression in preadipocytes at different stages of differentiation were analyzed using RNA sequencing. A total of 1,300,074,528 clean reads and 27,023 novel lncRNAs were obtained from 12 samples. The number of genes (1336 lncRNAs and 1759 mRNAs; 3095 in total) differentially expressed across various stages declined as differentiation progressed. Differentially expressed genes were found to be involved in several pathways related to preadipocyte differentiation that have been extensively studied, including glycerolipid metabolism, and the mammalian target of rapamycin, peroxisome proliferator-activated receptor, and mitogen-activated protein kinase signaling pathways. To our knowledge, some pathways are being reported for the first time, including the propanoate metabolism, fatty acid metabolism, and oxidative phosphorylation pathways. Furthermore, 3095 differentially expressed genes were clustered into eight clusters, and their expression patterns were determined through K-means clustering. Genes involved in the K2 cluster likely play important roles in preadipocyte differentiation. Six stage-specific modules related to A0 (day 0), A2 (day 2), and A6 (day 6) stages were identified, using weighted coexpression network analysis. Nine central, highly connected .genes in stage-specific modules were subsequently identified, including XLOC_068731, XLOC_022661, XLOC_045161, XLOC_070302, CHD6, LLGL1, NEURL1B, KLHL38, and ACTR6. This study provides a valuable resource for further study of chicken lncRNA and facilitates a better understanding of preadipocyte differentiation in the chicken

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“…However, more recent research has revealed active roles for adipocytes in fat mass regulation and nutrient homeostasis, in addition to other homeostatic processes (Trayhurn 2005;Rosen and Spiegelman 2006). Functional studies across a variety of species show that KLF7 inhibits preadipocyte differentiation (Kawamura et al 2006;Zhang et al 2013). APOC1 is associated not only with total adipose cells but also average adipocyte size (Jong et al 2001).…”

Section: The Adipocyte Cyclementioning

confidence: 99%

Genetics of Obesity

Frazier‐Wood¹,

Wang²

2015

Metabolic Syndrome

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All definitions of the metabolic syndrome include some form of obesity as one of the possible features. Body mass index (BMI) has a known genetic component, currently estimated to account for about 70 % of the population variance in weight status for non-syndromal obesity. Much research effort has been expended in trying to identify the specific genes which contain polymorphisms that account for individual differences in BMI. The largest genome-wide association study (GWAS) to date confirmed and identified almost 100 genes where variation contributes to BMI in individuals of European ancestry. This GWAS validated numerous variants which give insights into the pathophysiology of obesity centering on altered glucose and lipid metabolism and differences in adipocyte formation. The most novel contribution was identifying numerous genes expressed in the brain, which likely have behavioral consequences. This fits well with contemporary work showing that eating behaviors are a key, heritable contribution to common obesity. Future work will expand this putative gene list and continue to give insights into the etiology of common obesity which may one day contribute to individualized risk profiles and treatment options.

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Klf7 modulates the differentiation and proliferation of chicken preadipocyte

Cited by 63 publications

References 24 publications

Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Genetics of Obesity

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