Knockdown of Rap2B, a Ras Superfamily Protein, Inhibits Proliferation, Migration, and Invasion in Cervical Cancer Cells via Regulating the ERK1/2 Signaling Pathway

Li, Yinghua; Li, Songyi; Huang, Lili

doi:10.3727/096504017x14912172235777

Cited by 11 publications

(7 citation statements)

References 26 publications

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“…The poor prognosis of patients with advanced cervical cancer is largely attributed to intrinsic molecular changes (2,3). During the growth and metastasis of cervical cancer, various oncogenes and tumor suppressors have been demonstrated to serve key roles, and some of these are suggested to become potential therapeutic targets for cancer treatment (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Jiang

Hou

et al. 2018

Exp Ther Med

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Abstract. MicroRNA serve crucial roles in a variety of human cancer types. The miR-302-367 cluster has been reported to suppress the proliferation of cervical carcinoma cells through the novel target AKT1; however, the molecular mechanism of miR-302 in cervical cancer metastasis remains unclear. The present study aimed to investigate the clinical significance of miR-302-3p expression in cervical cancer, and to examine the regulatory mechanism of miR-302-3p in the malignant phenotypes of cervical cancer cells. The present data indicated that miR-302-3p was significantly downregulated in cervical cancer tissues compared with the level in adjacent non-tumor tissues, and low expression of miR-302-3p was significantly associated with node metastasis, advanced clinical stage, and poor prognosis in patients with cervical cancer. Restoration of miR-302-3p expression caused a significant reduction in cervical cancer cell migration and invasion. Defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was identified as a novel target gene of miR-302-3p, and miR-302-3p negatively regulated the mRNA and protein expression of DCUN1D1 in cervical cancer HeLa cells. Additionally, overexpression of DCUN1D1 rescued the effects of miR-302-3p on the migration and invasion of cervical cancer cells. Furthermore, DCUN1D1 was upregulated in cervical cancer tissues compared with the levels in adjacent tissues, and its high expression was associated with node metastasis, advanced clinical stage, and shorter survival time in patients with cervical cancer. Notably, a negative correlation between miR-302-3p and DCUN1D1 expression in cervical cancer tissues was observed. Taken together, the present study suggests that miR-302-3p serves a suppressive role in cervical cancer metastasis, partly at least, via directly targeting DCUN1D1. Therefore, miR-302-3p/DCUN1D1 may be a potential therapeutic target for cervical cancer treatment.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Jiang

Hou

et al. 2018

Exp Ther Med

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“…Starch and glycogen can serve as energy reserves to sustain life. In addition, they can combine with other molecules to form glycoproteins, glycolipids and so on, 29 which play key roles in many biological basic activities such as cancer migration, 30 cell fusion, 31 protein function regulation 32 and embryo development. 33 Consequently, the recognition of carbohydrates in the medical, cell biology and other life sciences is of great significance.…”

Section: Carbohydrates Boronic Acid Sensorsmentioning

confidence: 99%

Recent development of boronic acid-based fluorescent sensors

et al. 2018

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As Lewis acids, boronic acids can bind with 1,2-or 1,3-diols in aqueous solution reversibly and covalently to form five or six cyclic esters, thus resulting in significant fluorescence changes. Based on this phenomenon, boronic acid compounds have been well developed as sensors to recognize carbohydrates or other substances. Several reviews in this area have been reported before, however, novel boronic acid-based fluorescent sensors have emerged in large numbers in recent years. This paper reviews new boronbased sensors from the last five years that can detect carbohydrates such as glucose, ribose and sialyl Lewis A/X, and other substances including catecholamines, reactive oxygen species, and ionic compounds. And emerging electrochemically related fluorescent sensors and functionalized boronic acid as new materials including nanoparticles, smart polymer gels, and quantum dots were also involved.By summarizing and discussing these newly developed sensors, we expect new inspiration in the design of boronic acid-based fluorescent sensors.

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“…Then we further explored the downstream pathway of miR-1184, and we confirmed that miR-1184 could target RAP2B in BC. RAP2B has been reported to act as an oncogene, which increases cell proliferation, migration, and invasion in multiple tumors, like prostate cancer, hepatocellular carcinoma, and cervical cancer [33][34][35]. Furthermore, RAP2B promotes angiogenesis by activating the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/ vascular endothelial growth factor (VEGF) pathway in renal cell carcinoma [36].…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0008035 Knockdown Inhibits Bladder Cancer Progression through miR-1184/RAP2B Axis

Liu

Jiang

et al. 2023

Urol Int

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Introduction: Circular RNAs (circRNAs) are related to the pathogenesis and progression of bladder cancer (BC). This research aimed to investigate the role and mechanism of hsa_circ_0008035 (circ_0008035) in BC progression. Methods: Circ_0008035, microRNA (miR)-1,184, and Ras-related protein 2B (RAP2B) levels were examined in BC via quantitative real-time polymerase chain reaction and Western blotting. Cell Counting Kit-8, colony formation, 5-ethynyl-2′-deoxyuridine staining, flow cytometry, caspase-3 assay kit, transwell, and tube formation assays were conducted to estimate the effects of circ_0008035 on the malignant phenotypes of BC tumors. The interaction between RNAs and genes was evaluated via a dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model of BC in nude mice was established to estimate the effect of circ_0008035 in BC in vivo. Results: Circ_0008035 and RAP2B levels were upregulated, while miR-1184 abundance was downregulated in BC tissues and cells. Circ_0008035 knockdown constrained cell proliferation, migration, invasion and angiogenesis but promoted apoptosis in vitro. And circ_0008035 silencing curbed xenograft tumor growth in vivo. Circ_0008035 acted as a miRNA sponge for miR-1184. Circ_0008035 increased RAP2B expression by sponging miR-1184. MiR-1184 downregulation relieved the effects of circ_0008035 knockdown on BC progression. And RAP2B knockdown partly reversed the effects of miR-1184 overexpression on BC progression. Conclusion: Circ_0008035-mediated BC progression via regulating the miR-1184/RAP2B axis, providing a potential target for BC treatment.

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Knockdown of Rap2B, a Ras Superfamily Protein, Inhibits Proliferation, Migration, and Invasion in Cervical Cancer Cells via Regulating the ERK1/2 Signaling Pathway

Cited by 11 publications

References 26 publications

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Recent development of boronic acid-based fluorescent sensors

Hsa_circ_0008035 Knockdown Inhibits Bladder Cancer Progression through miR-1184/RAP2B Axis

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