Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

Wang, Yang; Huang, Hong; Li, Yang

doi:10.1080/21691401.2019.1601099

Cited by 26 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-486 can promote the cell proliferation and mobility of renal cell carcinoma and inhibit its apoptosis ( 31 ). Several miRNAs have also been identified as effective biomarkers for the prognosis and progression of OS ( 32 , 33 ). miR-1225-5p serves as a tumor suppressor in OS and inhibits the proliferation, migration and invasion of OS cells by targeting Sox9 ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

“…miR-1225-5p serves as a tumor suppressor in OS and inhibits the proliferation, migration and invasion of OS cells by targeting Sox9 ( 32 ). miR-384 is downregulated in OS tissues and cells compared to normal controls and upregulates stem loop binding protein and promotes the growth and metastasis of OS ( 33 ). A previous study reported the downregulation of miR-605 in OS, which indicates that miR-605 may play a role in the progression of OS ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

2020

Oncol Lett

View full text Add to dashboard Cite

Osteosarcoma (OS) is a type of primary bone tumor, which is one of the leading causes of cancer-related death. MicroRNA (miR)-605 has been demonstrated to act as a prognostic biomarker and therapeutic target in various cancers, such as breast cancer and non-small cell lung cancer, but its function in OS remains unclear. The aim of the present study was to investigate the prognostic value of miR-605 in patients with OS by evaluating its expression levels and to explore the biological function of miR-605 in OS progression. For this purpose, tumor tissues and adjacent normal tissues were collected from OS patients, and the expression of miR-605 in the collected tissues and OS MG63, U2OS, HOS, and SAOS-2 cell lines was detected by quantitative real-time PCR. The prognostic value of miR-605 was evaluated by Kaplan-Meier survival curves and Cox regression analysis. The effects of miR-605 on OS cell proliferation, migration and invasion were analyzed by the CCK-8 and transwell assays, respectively. The results of the present study revealed that miR-605 was significantly downregulated in OS tissues compared with adjacent normal tissues, which was associated with the clinical stage and distant metastasis of patients. Additionally, the downregulation of miR-605 predicted the poor prognosis of patients with OS and served as an independent prognostic indicator. The downregulation of miR-605 enhanced cell proliferation, migration, and invasion of OS cells, which suggested that miR-605 may be involved in the progression of OS. The findings of the present study provide a new therapeutic target for the treatment of patients with OS.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

2020

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…Over the past decade, numerous studies have evaluated the molecular mechanisms of OS. Multiple miRNAs and protein-coding genes have been described and identified as OS biomarkers [20–23]. However, the expression profile of miRNA and mRNA in human OS tissues remains unclear.…”

Section: Discussionmentioning

confidence: 99%

RNA Sequencing of Osteosarcoma Gene Expression Profile Revealed that miR-214-3p Facilitates Osteosarcoma Cell Proliferation via Targeting Ubiquinol-Cytochrome c Reductase Core Protein 1 (UQCRC1)

et al. 2019

View full text Add to dashboard Cite

Background Osteosarcoma (OS) is a common primary malignant bone tumor for which the molecular mechanisms remain unclear. Studies on coding and non-coding RNAs are needed to determine the molecular mechanism. Material/Methods To explore the potential roles of miRNAs and mRNA in OS, we determined the miRNA and mRNA expression profile of 3 pairs of OS and paracancerous tissues from patients with OS by sequencing and bioinformatics analysis. The expression levels of critical miRNAs and mRNAs were verified in 10 pairs of OS and paracancerous tissues. An miRNA inhibitor and mimics were used to investigate the interactions between miRNAs and target genes. The cell counting kit-8 assay was performed to evaluate OS cell proliferation after miRNA interference. Results A total of 184 miRNAs and 2501 mRNAs were identified (fold-change >2.0 or <2.0, P<0.05), with up-regulation of 82 miRNAs and 1320 mRNAs and down-regulation of 102 miRNAs and 1181 mRNAs in OS tissue. The protein protein interaction network revealed that UQCRC1 (ubiquinol-cytochrome c reductase core protein 1) is a critical gene and a potential target gene of miR-214-3p. Both UQCRC1 and miR-214-3p were significantly differentially expressed in OS tissue and cell lines (down and up-regulated, respectively). Down-regulated miR-214-3p expression increased UQCRC1 expression and suppressed OS cell proliferation. In contrast, overexpression of miR-214-3p decreased UQCRC1 expression and promoted OS cell proliferation. Conclusions High miR-214-3p expression may promote OS cell proliferation by targeting UQCRC1, providing insight into a potential therapeutic target for preventing and treating OS.

show abstract

“…oS demonstrates a high probability to metastasize and damage surrounding tissues, especially within the lung; however, despite the existence of surgical excision and the development of neoadjuvant chemotherapies for patients with oS over the past few decades, the prognosis of oS for 20 year survival remains <20% (3). This poor prognosis is largely due to the anatomical location, tumor size, tumor stage, presence or absence of local recurrence and metastasis, in addition to ineffective chemotherapy regimens (4). Thus, the development of new patient treatment strategies for OS is urgently required.…”

Section: Introductionmentioning

confidence: 99%

Suppression of CLEC3A inhibits osteosarcoma cell proliferation and promotes their chemosensitivity through the AKT1/mTOR/HIF1α signaling pathway

Ren¹,

Pan²,

Hou³

et al. 2020

Mol Med Report

View full text Add to dashboard Cite

osteosarcoma (oS) is a primary malignant tumor that occurs in bone, and mainly affects children and adolescents. c-type lectin domain family 3 member a (clec3a) is a member of the c-type lectin superfamily, which regulates various biological functions of cells. The present study aimed to identify the effects and related mechanisms of clec3a in the proliferation and chemosensitivity of oS cells. The expression of clec3a in oS was analyzed using the Gene Expression Omnibus data profile GSE99671, and its expression in OS samples was verified using reverse transcription-quantitative PCR (RT-qPCR) and immunohistochemical staining. The relationship between the expression of clec3a and clinical traits in patients with oS was also analyzed, including age, tumor size, TnM stage and lymph node metastasis. cell counting Kit-8 assays, colony formation assays and cell cycle distribution analysis were used to determine the roles of clec3a in the proliferation and chemosensitivity of OS cells. Finally, RT-qPCR and western blotting were used to demonstrate the relationship between clec3a and the aKT1/mTor/hypoxia-inducible factor 1-α (HiF1α) pathway. Both the mrna and protein expression levels of clec3a were increased in oS tissues compared with adjacent non-tumor tissues, and this was positively associated with TnM stage and lymph node metastasis. The genetic knockdown of clec3a with small interfering rna decreased oS cell proliferation and colony formation, and induced G1 phase arrest, whereas the overexpression of clec3a increased oS cell proliferation and colony formation, and alleviated G1 phase arrest. The suppression of clec3a also promoted enhanced the chemosensitivity of oS cells to doxorubicin (doX) and cisplatin (cddP); it also inhibited the expression of aKT1, mTor and HiF1α, further to the nuclear localization of HiF1α, and HiF1α target gene expression levels, including VeGF, GluT1 and Mcl1 were also decreased. Furthermore, treatment with the aKT activator Sc79 blocked the inhibitory effects of clec3a silencing in oS cells. in conclusion, these findings suggested that CLEC3A may function as an oncogene in oS, and that the suppression of clec3a may inhibit oS cell proliferation and promote chemosensitivity through the aKT1/mTor/HiF1α signaling pathway.

show abstract

Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

Cited by 26 publications

References 34 publications

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

RNA Sequencing of Osteosarcoma Gene Expression Profile Revealed that miR-214-3p Facilitates Osteosarcoma Cell Proliferation via Targeting Ubiquinol-Cytochrome c Reductase Core Protein 1 (UQCRC1)

Suppression of CLEC3A inhibits osteosarcoma cell proliferation and promotes their chemosensitivity through the AKT1/mTOR/HIF1α signaling pathway

Contact Info

Product

Resources

About