Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Roberts, Alison W.; Dimos, Christos S.; Goss, Chessa A.; Lai, Virginia; Chaves, Arielle M.

doi:10.1007/978-1-0716-0621-6_8

Methods in Molecular Biology

2020

DOI: 10.1007/978-1-0716-0621-6_8

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Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Alison W. Roberts¹,

Christos S. Dimos²,

Chessa A. Goss³

et al.

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2024

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Cited by 1 publication

References 69 publications

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An alternate route for cellulose microfibril biosynthesis in plants

Roberts,

Yuan,

Chaves

et al. 2024

Sci. Adv.

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Similar to cellulose synthases (CESAs), cellulose synthase–like D (CSLD) proteins synthesize β-1,4-glucan in plants. CSLDs are important for tip growth and cytokinesis, but it was unknown whether they form membrane complexes in vivo or produce microfibrillar cellulose. We produced viable CESA-deficient mutants of the moss Physcomitrium patens to investigate CSLD function without interfering CESA activity. Microscopy and spectroscopy showed that CESA-deficient mutants synthesize cellulose microfibrils that are indistinguishable from those in vascular plants. Correspondingly, freeze-fracture electron microscopy revealed rosette-shaped particle assemblies in the plasma membrane that are indistinguishable from CESA-containing rosette cellulose synthesis complexes (CSCs). Our data show that proteins other than CESAs, most likely CSLDs, produce cellulose microfibrils in P. patens protonemal filaments. The data suggest that the specialized roles of CSLDs in cytokinesis and tip growth are based on differential expression and different interactions with microtubules and possibly Ca 2+ , rather than structural differences in the microfibrils they produce.

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An alternate route for cellulose microfibril biosynthesis in plants

Roberts,

Yuan,

Chaves

et al. 2024

Sci. Adv.

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show abstract

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Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Cited by 1 publication

References 69 publications

An alternate route for cellulose microfibril biosynthesis in plants

An alternate route for cellulose microfibril biosynthesis in plants

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