Kök Hücrelerde DNA Hasarı ve Onarımı DNA Damage and Repair In Stem Cells

DURMAZ, Ceyda; ŞEN, Esra

doi:10.17932/iau.tfk.2018.008/tfk_v05i1003

Cited by 2 publications

(2 citation statements)

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“…These include growth factors, the extracellular environment, cell-cell signalling, inflammatory mediators and cellular metabolism. However, stem cells are exposed to harmful effects in the case of disease or during the ageing process [6].…”

Section: Discussionmentioning

confidence: 99%

Adipoz dokudan izole edilen mezenkimal kök hücrelerin donör yaşına bağlı olarak mTOR aktivitesinin belirlenmesi

SARIKAYA,

ÖNDER,

ÇİL

et al. 2023

Pam Tıp Derg

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Purpose: Aging in living organisms is an inevitable physiological consequence. Cellular senescence occurs not only in cells that have completed their differentiation, but also in stem cells. Mammalian target of Rapamycin protein complex (mTOR) has an important role in cell growth and metabolism. mTOR, which plays an important role in cell proliferation, also regulates cellular aging and directs the bionergetic infrastructure. The aim of the study is to determine the mTOR expression of mesenchymal stem cell (MSC) obtained from adipose tissue depending on the donor age. Materials and methods: 6-week-old pubertal rats were named Group 1 (n=6), 10-12-week-old reproductive period rats were named Group 2 (n=6), and 20-month-old rats were named Group 3 (n=6). MSC isolation was performed by primary explant culture method from adipose tissue taken from groups. Characterization and differentiation experiments were performed in MSC obtained. mTOR activity (mTORC1 and mTORC2) in MSC was determined by RT-PCR method. Caspase 3, 8, 9, Bax and Bcl-2 expressions were evaluated by RT-PCR method. Results: In our study, it was determined that the highest expression of apoptotic markers was in Group 1 and the lowest expression was in Group 2. When mTOR expression was evaluated, mTORC1 was found to be highest in Group 2 and lowest in Group 1. mTORC2 expression in Group 1 was lower than in other groups. Although the expression of mTORC1 and mTORC2 in Group 3 was not as high as in Group 2, it was statistically significant (p

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Section: Discussionmentioning

confidence: 99%

Adipoz dokudan izole edilen mezenkimal kök hücrelerin donör yaşına bağlı olarak mTOR aktivitesinin belirlenmesi

SARIKAYA,

ÖNDER,

ÇİL

et al. 2023

Pam Tıp Derg

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“…On the other hand, Magnesium (Mg) is an essential mineral that plays a vital role in various biochemical reactions within the body. It is involved in regulating crucial functions such as nervous system activity, muscle contractions, energy production, protein synthesis, and DNA replication [3]. In this study, the researchers opted for the SDD basis set in conjunction with the DFT method to analyze the Norepinephrine molecule most effectively.…”

Section: Introductionmentioning

confidence: 99%

Investigation of UV-Visible Absorption Quantum Effects Doped of Norepinephrine, Mg+2 Atom by Using DFT Method

KEBİROGLU,

YILMAZ

2023

Journal of Physical Chemistry and Functional Materials

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The research focused on investigating and optimizing the interaction of the hormone Norepinephrine (Noradrenaline), which acts as a neurotransmitter, with Mg+2 atoms at the molecular level. To do this, we used GaussView 6.0.16 to generate the molecular structure. Then, it employed Gaussian 09: AS64L-G09RevD.01 program to optimize the molecular structures of Norepinephrine using the DFT method and SDD basis set, and the Mg atom doped molecule using the DFT method and LanL2MB basis set. Various quantum mechanical calculations were conducted on the molecule, including Fourier Transform Infrared spectroscopy (FT-IR), Nuclear Magnetic Resonance Spectroscopy (NMR), HOMO-LUMO structure with the energy level diagram, UV-visible absorption, and density of states (DOS). These calculations provide important insights into the behavior and properties of the Norepinephrine-Mg+2 complex at the molecular level.

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Kök Hücrelerde DNA Hasarı ve Onarımı DNA Damage and Repair In Stem Cells

Cited by 2 publications

References 0 publications

Adipoz dokudan izole edilen mezenkimal kök hücrelerin donör yaşına bağlı olarak mTOR aktivitesinin belirlenmesi

Adipoz dokudan izole edilen mezenkimal kök hücrelerin donör yaşına bağlı olarak mTOR aktivitesinin belirlenmesi

Investigation of UV-Visible Absorption Quantum Effects Doped of Norepinephrine, Mg+2 Atom by Using DFT Method

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