Kunzea Ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-melanogenesis Effects in B16F10 Cells

Abstract

The present study identi ed various compounds, such as chlorogenic acid, gallic acid, quercetin, and (E)-Ferulic acid in the kunzea ericoides (kanuka) leaf extract, showing its potential use in maintaining skin health. The in uence of kanuka leaf extract upon epidermal cells concerning cytotoxicity and in vitro activities of moisturisation, anti-oxidation, UVB protection, and anti-melanogenesis effects were explored in the study. Kanuka leaf extract demonstrated signi cant promotion in the proliferation of HaCaT and B16F10 cells. After incubation with kanuka leaf extract, the content of ROS and DPPH in HaCaT was signi cantly decreased; at the same time, more SOD was produced. Furthermore, hyaluronidase-1 (HYAL-1) and HYAL-4 expressions were inhibited, while the aquaporin 3 (AQP-3) content was signi cantly increased. Kanuka leaf extract also inhibited the expressions of matrix metalloproteinases-1 (MMP-1) and MMP-14 in UVB radiated-HaCaT cells. In the B16F10 cell line, melanin and tyrosinase production were decreased under the presence of kanuka leaf extract, and the expressions of microphthalmiaassociated transcription factor (MITF), tyrosinase-related protein-1 (TYRP-1) and TYRP-2 were also inhibited.