Kurze interferierende RNA und ihre chemische Synthese

Characterization of Modified RNA by Top‐Down Mass Spectrometry

“…Chemicals and tRNA Val (from Escherichia coli , GGGUG AU s 4 U AG CUCAG C D GGG AGAGC ACCUC CCU cmo 5 U A C m 6 A AGG AGGGG m 7 G UCGG CGG TΨ CGAUC CCGUC AUCAC CCACC A, modified residues in bold; s 4 U : 4-thiouridine, D : dihydrouridine, cmo 5 U : uridine-5-oxyacetic acid, m 6 A : N 6 -methyladenosine, m 7 G : 7-methylguanosine, T : thymidine, Ψ : pseudouridine) were purchased from Sigma–Aldrich (Austria). The 22 nt RNA (GGACG AUACG CGUGA AGCGU CC) was prepared by solid-phase synthesis 20. For increased statistics, 500 scans were added for each spectrum.…”

On Secondary Structure Rearrangements and Equilibria of Small RNAs

“…Single‐nucleotide labeling of RNA is still a major challenge and highly demanding from a chemical point of view. However, recent improvements in the chemical synthesis of oligoribonucleotides are promising and may make site‐specifically labeled RNAs readily available soon 62–66…”

A General Approach for the Identification of Site‐Specific RNA Binders by 19F NMR Spectroscopy: Proof of Concept

“…The recent improvements in the chemical synthesis of RNA make ribose 2'-F labeling available for all four standard nucleobases, and significantly expands the scope of promising applications in RNA research. [34] One example is the verification of RNA folding equilibria that we have reported recently. [35] The approach presented here is another application.…”