l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase

Kwack, Mi Hee; Shin, Sug Kyun; Kim, S.R.; Im, Sang Uk; Han, In Mee; Kim, M.K.; Kim, J.C.; Sung, Young Kwan

doi:10.1111/j.1365-2133.2009.09108.x

Cited by 34 publications

(36 citation statements)

References 24 publications

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“…Anagen hair follicles were isolated from a volunteer and cultured ex vivo, as previously described [28,29]. Briefly, dissected hair follicles were cut into small pieces (*2 mm in length from the bottom of the dermal papilla) and cultured in Williams E medium (Sigma) with 10 ng/mL hydrocortisone, 10 ng/mL insulin, 2 mM l-glutamine, and 100 U/mL penicillin at 37°C in 5% CO 2 .…”

Section: Hair Organ Culturementioning

confidence: 99%

The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells

Kim

Sung

et al. 2014

Stem Cells and Development

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Although adipose-derived stem cells (ASCs) show promise for cell therapy, there is a tremendous need for developing ASC activators. In the present study, we investigated whether or not vitamin C increases the survival, proliferation, and hair-regenerative potential of ASCs. In addition, we tried to find the molecular mechanisms underlying the vitamin C-mediated stimulation of ASCs. Sodium-dependent vitamin C transporter 2 (SVCT2) is expressed in ASCs, and mediates uptake of vitamin C into ASCs. Vitamin C increased the survival and proliferation of ASCs in a dose-dependent manner. Vitamin C increased ERK1/2 phosphorylation, and inhibition of the mitogen-activated protein kinase (MAPK) pathway attenuated the proliferation of ASCs. Microarray and quantitative polymerase chain reaction showed that vitamin C primarily upregulated expression of proliferation-related genes, including Fos, E2F2, Ier2, Mybl1, Cdc45, JunB, FosB, and Cdca5, whereas Fos knock-down using siRNA significantly decreased vitamin C-mediated ASC proliferation. In addition, vitamin C-treated ASCs accelerated the telogen-to-anagen transition in C 3 H/HeN mice, and conditioned medium from vitamin C-treated ASCs increased the hair length and the Ki 67 -positive matrix keratinocytes in hair organ culture. Vitamin C increased the mRNA expression of HGF, IGFBP6, VEGF, bFGF, and KGF, which may mediate hair growth promotion. In summary, vitamin C is transported via SVCT2, and increased ASC proliferation is mediated by the MAPK pathway. In addition, vitamin C preconditioning enhanced the hair growth promoting effect of ASCs. Because vitamin C is safe and effective, it could be used to increase the yield and regenerative potential of ASCs.

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Section: Hair Organ Culturementioning

confidence: 99%

The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells

Kim

Sung

et al. 2014

Stem Cells and Development

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“…Immunofluorescence staining of Ki-67 was performed as described previously [15]. Briefly, hair follicle specimen block was cut a thickness of 7 mm sections.…”

Section: Immunofluorescence Staining Of Ki-67mentioning

confidence: 99%

Erythropoietin promotes hair shaft growth in cultured human hair follicles and modulates hair growth in mice

Kang

Shin

Kwack

et al. 2010

Journal of Dermatological Science

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“…Human follicle dermal papilla (DP) cells were obtained from PromoCell and cultured in a Follicle Dermal Papilla Cell Growth Medium with a Supplement Mix (PromoCell) at 37°C in a 5% CO 2 /95% air humidified atmosphere. Outer root sheath (ORS) cells were obtained and cultured as described previously [16] in an EpiLife Medium (Gibco/Invitrogen) with supplement mixtures plus penicillin-streptomycin in 5% CO 2 at 37°C.…”

Section: Cell Culturesmentioning

confidence: 99%

“…Anagen hair follicles (HFs) were isolated from volunteer and cultured ex vivo, as described previously [16,20]. Briefly, dissected HFs were cut into small pieces (*2 mm in length from the bottom of the DP) and cultured in the Williams E medium (Gibco BRL) with 10 ng/mL hydrocortisone, 10 ng/ mL insulin, 2 mM l-glutamine, and 100 U/mL penicillin at 37°C in 5% CO 2 .…”

Section: Hair Organ Culturementioning

confidence: 99%

Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

Jeong

Sung

Kim

et al. 2013

Stem Cells and Development

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Hypoxia induces the survival and regenerative potential of adipose-derived stem cells (ASCs), but there are tremendous needs to find alternative methods for ASC preconditioning. Therefore, this work investigated: (1) the ability of low-dose ultraviolet B (UVB) radiation to stimulate the survival, migration, and tube-forming activity of ASCs in vitro; (2) the ability of UVB preconditioning to enhance the hair growth-promoting capacity of ASCs in vivo; and (3) the mechanism of action for ASC stimulation by UVB. Although high-dose UVB decreased the proliferation of ASCs, low-dose (10 or 20 mJ/cm 2 ) treatment increased their survival, migration, and tube-forming activity. In addition, low-dose UVB upregulated the expression of ASC-derived growth factors, and a culture medium conditioned by UVB-irradiated ASCs increased the proliferation of dermal papilla and outer root sheet cells. Notably, injection of UVB-preconditioned ASCs into C 3 H/HeN mice significantly induced the telogen-to-anagen transition and increased new hair weight in vivo. UVB treatment significantly increased the generation of reactive oxygen species (ROS) in cultured ASCs, and inhibition of ROS generation by diphenyleneiodonium chloride (DPI) significantly attenuated UVB-induced ASC stimulation. Furthermore, NADPH oxidase 4 (Nox4) expression was induced in ASCs by UVB irradiation, and Nox4 silencing by small interfering RNA, like DPI, significantly reduced UVB-induced ROS generation. These results suggest that the primary involvement of ROS generation in UVB-mediated ASC stimulation occurs via the Nox4 enzyme. This is the first indication that a low dose of UVB radiation and/or the control of ROS generation could potentially be incorporated into a novel ASC preconditioning method for hair regeneration.

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l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase

Abstract: These data show that Asc 2-P-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K.

Cited by 34 publications

References 24 publications

The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells

The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells

Erythropoietin promotes hair shaft growth in cultured human hair follicles and modulates hair growth in mice

Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

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