L-Asparaginase – A prospective anticancer enzyme on MCF-7 cell lines extracted from soil Microflora

Abstract

This study aims at investigating the soil bacteria for L asparaginase enzyme production and its anti-cancer effect on MCF 7 cell line. In this study, 30 soil samples were screened for bacterial producing L asparaginase enzyme. The soil samples were collected from various places in Ethiraj College, Chennai. Isolation was done using spread plate technique, biochemical and microscopic characterization for the potential isolates revealed the bacterial genera as Bacillus, Escherichia and Pseudomonas species. Of the thirty samples collected, six isolates produced pronounced L asparaginase enzyme. These samples were further subjected to crude enzyme extraction and estimation. The isolates S2 and Ni5 showed maximum production of enzyme with 1090 units/ml of activity. Dialysis was carried out to concentrate the enzyme and molecular characterization was performed using SDS PAGE to separate the proteins according to the molecular weight which was found to be approximately 100 to 116 kDa. Anti-cancer activity was evaluated on MCF 7 tumour cell line. The cell viability was dose dependent and IC50 of S2 was 62.5 μg and Ni5 31.2 μg respectively.